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Cultivation method of heat-resistant zooxanthellae

A kind of cultivation method and technology of zooxanthellae, applied in the field of cultivation of heat-resistant zooxanthellae

Inactive Publication Date: 2019-10-25
浙江山诺生物科技有限公司
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Problems solved by technology

However, this hypothesis has not yet been confirmed. It is still a mystery whether the corals expelled the zooxanthellae, or the zooxanthellae left because they found that the environmental conditions were unfavorable.

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  • Cultivation method of heat-resistant zooxanthellae
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  • Cultivation method of heat-resistant zooxanthellae

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Embodiment

[0025] like Figure 1-3 shown

[0026] A method for cultivating heat-resistant zooxanthellae, which specifically comprises the following steps,

[0027] (1) Inoculation: peel off the healthy zooxanthellae from the symbiotic film of the coral and inoculate in the photosynthetic bioreactor group;

[0028] (2) Cultivation: inoculate the healthy zooxanthellae obtained in step (1) into the photosynthetic biochemical reactor group for individual cultivation;

[0029] (3) Oriented evolution: Cultivate until the zooxanthellae enter the logarithmic growth phase, put them in a hot water bath at 45°C for heat shock for 2 hours, and wait for the zooxanthellae to begin to bleach; then put them back into the 25°C photosynthetic bioreactor Inside, after the complete albino phenomenon occurs, centrifuge the albino zooxanthellae cells, pour off the supernatant, and use fresh f / 2 artificial seawater culture medium to cultivate for standby;

[0030] (4) Repeat the above steps while gradually ...

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Abstract

The invention belongs to the technical field of alga cultivation and particularly discloses a cultivation method of heat-resistant zooxanthellae. The cultivation method specifically includes the stepsof firstly, inoculating, to be more specific, inoculating healthy zooxanthellae into a photobioreactor set; secondly, culturing, to be more specific, inoculating healthy zooxanthellae obtained in thefirst step into a photobioreactor set to perform independent culturing; thirdly, performing directed evolution; fourthly, repeating the steps above, and gradually increasing temperature and heat shock time at the same time to allow synechocystis mutant strains to be able to normally grow at the temperature larger than 45 DEG C. The cultivation method has the advantages that the directed evolutionis used to modify the zooxanthellae, the obtained synechocystis mutant strains can normally grow at the temperature larger than 45 DEG C, and the zooxanthellae is good in heat resistance.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and specifically discloses a cultivation method of heat-resistant zooxanthellae. Background technique [0002] Dinoflagellates are one of the most important primary producers in marine ecosystems. Among them, zooxanthellae (Symbiodinium) are essential symbiotic algae in coral reefs. The discovery of zooxanthellae is more than 100 years old. In 1881, German scientist Brandt discovered that a golden-yellow single-celled algae co-exists among different radiolarians, polyps and sea anemones, which he suggested to be called "zooxanthellae". At first, scientists called the diatoms and dinoflagellates that symbiotic with spiralworms and yellow-sidered sea anemones as zooxanthellae, and later the unicellular algae that symbiotic with jellyfish, sea anemones, corals, polyps and even the mantle of clam clam are collectively referred to as zooxanthellae. For zooxanthellae. Zooxanthellae was or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 傅鹏程
Owner 浙江山诺生物科技有限公司
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