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Duck protein derived peptide YSPAF having ACE and HMG-CoA reductase restraining activity and purpose

A technology that inhibits activity and reductase, and is used in peptides, metabolic diseases, cardiovascular system diseases, etc.

Inactive Publication Date: 2019-10-29
INNER MONGOLIA SAIFEIYA AGRI SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] According to existing drug reports, except for traditional Chinese medicines, drugs that have both blood pressure and blood fat lowering effects are rare

Method used

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  • Duck protein derived peptide YSPAF having ACE and HMG-CoA reductase restraining activity and purpose
  • Duck protein derived peptide YSPAF having ACE and HMG-CoA reductase restraining activity and purpose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] ACE inhibitory activity of 5-peptide YSPAF at a concentration of 1.0 mg / mL:

[0059] Chromatographic conditions: Solvent I is 0.05% trifluoroacetic acid (TFA) and 0.05% triethylamine (TTA) dissolved in deionized water (that is, every liter of solvent I contains 0.5mL of trifluoroacetic acid and 0.5mL of triethylamine ), solvent II is 100% chromatographically pure acetonitrile. The ratio of solvent Ⅰ to solvent Ⅱ is 70%: 30% (volume ratio), ultimate3000 Diane liquid chromatograph, the chromatographic column is waters Symmetry C 18 5μm 4.6×250mm, the flow rate is 0.5mL / min, the injection volume is 10μL, the detection wavelength is 225nm, and the detection column temperature is 30°C.

[0060] Detection method: the pentapeptide YSPAF will be obtained through chemical synthesis, and the activity detection will be carried out (the detection method is the same as above). At this time, the concentration of YSPAF was 1.0 mg / mL.

[0061] Results: The ACE inhibitory activity o...

Embodiment 2

[0067] ACE inhibitory activity of 5-peptide YSPAF at a concentration of 2.0 mg / mL:

[0068] Chromatographic conditions: Solvent I is 0.05% trifluoroacetic acid (TFA) and 0.05% triethylamine (TTA) dissolved in deionized water; solvent II is 100% chromatographically pure acetonitrile. The ratio of solvent Ⅰ to solvent Ⅱ is 70%: 30%, the ultimate3000 Diane liquid chromatograph, the chromatographic column is waters Symmetry C 18 5μm 4.6×250mm, the flow rate is 0.5mL / min, the injection volume is 10μL, the detection wavelength is 225nm, and the detection column temperature is 30°C.

[0069] Detection method: the pentapeptide YSPAF will be obtained through chemical synthesis, and the activity detection will be carried out (the detection method is the same as above). At this time, the concentration of YSPAF was 2.0 mg / mL.

[0070] Results: The ACE inhibitory activity of pentapeptide YSPAF at 2.0 mg / mL was 85.24%.

[0071] HMG-CoA reductase inhibitory activity of 5-peptide YSPAF at...

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PUM

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Abstract

The invention belongs to the technical field of biology, and particularly relates to 5 peptide which can be combined with an angiotensin converting enzyme to restrain activity of the angiotensin converting enzyme and can also restrain activity of 3-hydroxy-3-methyl glutaryl coenzyme A (HMG-CoA) reductase. The invention discloses duck protein derived peptide YSPAF having ACE and HMG-CoA reductase restraining activity, and the amino acid sequence of the peptide YSPAF is Tyr-Ser-Pro-Ala-Phe. At the same time, the invention further discloses an application of the peptide YSPAF to preparation of ACE restraining peptide and HMG-CoA reductase restraining peptide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to an angiotensin-converting enzyme that can bind to angiotensin-converting enzyme, inhibit its activity, and can also inhibit the activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase 5 peptides. Background technique [0002] Angiotensin converting enzyme (Angiotensin converting enzyme, ACE, EC3.4.15.1, once used in the literature as kininaseIl, dipeptidyl carboxypeptidase I, etc.) is a dicarboxypeptidase, which is a key enzyme leading to high blood pressure. It passes Hydrolysis converts angiotensin I to angiotensin II, and at the same time, ACE can also inactivate bradykinin, both of which can lead to vasoconstriction and thus hypertension. Therefore, ACE is considered to be an important factor causing hypertension. Studies have found that angiotensin-converting enzyme inhibitors (ACEI) can lower blood pressure by inhibiting the activity of ACE. ACE inhi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61P3/06A61P9/12
CPCA61P3/06A61P9/12C07K7/06
Inventor 李槟全王伟赵秉和杨华张玉王君虹李雪朱作艺孙素玲
Owner INNER MONGOLIA SAIFEIYA AGRI SCI & TECH DEV
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