Hexapeptide QYPVEK having ACE and HMG-CoA reductase restraining activity and application
A technology for inhibiting activity and reductase, applied in the field of active 6-peptide
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Embodiment 1
[0057] ACE inhibitory activity of 6-peptide QYPVEK at a concentration of 1.0 mg / mL:
[0058] Chromatographic conditions: Solvent I is 0.05% trifluoroacetic acid (TFA) and 0.05% triethylamine (TTA) dissolved in deionized water (that is, every liter of solvent I contains 0.5mL of trifluoroacetic acid and 0.5mL of triethylamine ), solvent II is 100% chromatographically pure acetonitrile. The ratio of solvent Ⅰ to solvent Ⅱ is 70%: 30% (volume ratio), ultimate3000 Diane liquid chromatograph, the chromatographic column is watersSymmetry C 18 5μm 4.6×250mm, the flow rate is 0.5mL / min, the injection volume is 10μL, the detection wavelength is 225nm, and the detection column temperature is 30°C.
[0059] Detection method: The hexapeptide QYPVEK structure will be obtained through chemical synthesis, and the activity detection will be carried out (the detection method is the same as above). At this time, the concentration of QYPVEK was 1.0 mg / mL.
[0060] Results: The ACE inhibitory...
Embodiment 2
[0066] ACE inhibitory activity of 6-peptide QYPVEK at a concentration of 2.0 mg / mL:
[0067] Chromatographic conditions: Solvent I is 0.05% trifluoroacetic acid (TFA) and 0.05% triethylamine (TTA) dissolved in deionized water; solvent II is 100% chromatographically pure acetonitrile. The ratio of solvent Ⅰ to solvent Ⅱ is 70%: 30%, the ultimate3000 Diane liquid chromatograph, the chromatographic column is waters Symmetry C 18 5μm 4.6×250mm, the flow rate is 0.5mL / min, the injection volume is 10μL, the detection wavelength is 225nm, and the detection column temperature is 30°C.
[0068] Detection method: the hexapeptide will be obtained through chemical synthesis, and the activity detection will be carried out (the detection method is the same as above). At this time, the DA concentration was 2.0 mg / mL.
[0069] Results: The ACE inhibitory activity of the 6-peptide QYPVEK at 2.0 mg / mL was 93.14%.
[0070] HMG-CoA reductase inhibitory activity of 6-peptide QYPVEK at a concen...
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