Hexapeptide QYPVEK having ACE and HMG-CoA reductase restraining activity and application

A technology for inhibiting activity and reductase, applied in the field of active 6-peptide

Inactive Publication Date: 2019-11-26
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] According to existing drug reports, except for traditional Chinese medicines, drugs that have both blood pressure and blood fat lowering effects are rare

Method used

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  • Hexapeptide QYPVEK having ACE and HMG-CoA reductase restraining activity and application
  • Hexapeptide QYPVEK having ACE and HMG-CoA reductase restraining activity and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] ACE inhibitory activity of 6-peptide QYPVEK at a concentration of 1.0 mg / mL:

[0058] Chromatographic conditions: Solvent I is 0.05% trifluoroacetic acid (TFA) and 0.05% triethylamine (TTA) dissolved in deionized water (that is, every liter of solvent I contains 0.5mL of trifluoroacetic acid and 0.5mL of triethylamine ), solvent II is 100% chromatographically pure acetonitrile. The ratio of solvent Ⅰ to solvent Ⅱ is 70%: 30% (volume ratio), ultimate3000 Diane liquid chromatograph, the chromatographic column is watersSymmetry C 18 5μm 4.6×250mm, the flow rate is 0.5mL / min, the injection volume is 10μL, the detection wavelength is 225nm, and the detection column temperature is 30°C.

[0059] Detection method: The hexapeptide QYPVEK structure will be obtained through chemical synthesis, and the activity detection will be carried out (the detection method is the same as above). At this time, the concentration of QYPVEK was 1.0 mg / mL.

[0060] Results: The ACE inhibitory...

Embodiment 2

[0066] ACE inhibitory activity of 6-peptide QYPVEK at a concentration of 2.0 mg / mL:

[0067] Chromatographic conditions: Solvent I is 0.05% trifluoroacetic acid (TFA) and 0.05% triethylamine (TTA) dissolved in deionized water; solvent II is 100% chromatographically pure acetonitrile. The ratio of solvent Ⅰ to solvent Ⅱ is 70%: 30%, the ultimate3000 Diane liquid chromatograph, the chromatographic column is waters Symmetry C 18 5μm 4.6×250mm, the flow rate is 0.5mL / min, the injection volume is 10μL, the detection wavelength is 225nm, and the detection column temperature is 30°C.

[0068] Detection method: the hexapeptide will be obtained through chemical synthesis, and the activity detection will be carried out (the detection method is the same as above). At this time, the DA concentration was 2.0 mg / mL.

[0069] Results: The ACE inhibitory activity of the 6-peptide QYPVEK at 2.0 mg / mL was 93.14%.

[0070] HMG-CoA reductase inhibitory activity of 6-peptide QYPVEK at a concen...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to hexapeptide which can be combined with an angiotensin converting enzyme to restrain activity of the angiotensin converting enzyme and can also restrain activity of 3-hydroxy-3-methyl glutaryl coenzyme A (HMG-CoA) reductase. The invention discloses hexapeptide QYPVEK having ACE and HMG-CoA reductase restraining activity, and the amino acid sequence of the hexapeptide QYPVEK is Gln-Tyr-Pro-Val-Glu-Lys. Besides, the invention also discloses an application of the hexapeptide QYPVEK to preparation of ACE restraining peptide and HMG-CoA reductase restraining peptide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to an angiotensin-converting enzyme that can be combined to inhibit its activity, and can also inhibit the activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase of 6 peptides. Background technique [0002] Angiotensin converting enzyme (Angiotensin converting enzyme, ACE, EC3.4.15.1, once used in the literature as kininaseIl, dipeptidyl carboxypeptidase I, etc.) is a dicarboxypeptidase, which is a key enzyme leading to high blood pressure. It passes Hydrolysis converts angiotensin I to angiotensin II, and at the same time, ACE can also inactivate bradykinin, both of which can lead to vasoconstriction and thus hypertension. Therefore, ACE is considered to be an important factor causing hypertension. Studies have found that angiotensin-converting enzyme inhibitors (ACEI) can lower blood pressure by inhibiting the activity of ACE. ACE inhibitors are widely use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61P9/00A61P9/12A61P9/06A61P13/12
CPCA61K38/00A61P9/00A61P9/06A61P9/12A61P13/12C07K7/06
Inventor 王伟杨华李槟全张玉王君虹李雪朱作艺孙素玲
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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