PSMA-targeting single-strand antibody, and PSMA-targeting chimeric antigen receptor T cell and preparation method and application thereof

A technology of chimeric antigen receptors and single-chain antibodies, which is applied in the field of medical biology, can solve problems such as limitations and lack of relevant research on a variety of PSMA-positive solid tumors, and achieve highly tissue-specific expression effects

Inactive Publication Date: 2019-12-03
SHENZHEN BINDEBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current application of CART technology is still limited to hematological tumors, and there is no relevant research on the application of various PSMA-positive solid tumors.

Method used

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  • PSMA-targeting single-strand antibody, and PSMA-targeting chimeric antigen receptor T cell and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] A method for preparing chimeric antigen receptor T cells targeting PSMA, comprising the following steps:

[0078] (1) Prepare the gene sequence of the chimeric antigen receptor CAR-PSMA targeting PSMA

[0079] The coding genes of the signal peptide, the single-chain antibody targeting PSMA, the CD8α hinge region, the CD8 transmembrane region, the 4-1BB signal region and the CD3ζ signal region are respectively prepared, and the coding genes of the signal peptide are shown in SEQ ID NO: 15 , the coding gene of the single-chain antibody targeting PSMA is shown in SEQ ID NO: 2, the coding gene of the CD8α hinge region is shown in SEQ ID NO: 7, and the coding gene of the CD8 transmembrane region is shown in SEQ ID NO: As shown in ID NO: 9, the gene encoding the 4-1BB signal region is shown in SEQ ID NO: 11, and the gene encoding the CD3ζ signal region is shown in SEQ ID NO: 13.

[0080] The genes encoding the above signal peptide, PSMA-targeting single-chain antibody, CD8α hi...

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Abstract

The invention provides a PSMA-targeting single-strand antibody. The PSMA-targeting single-strand antibody comprises an amino acid sequence as shown in SEQ ID NO:1. The invention further provides a chimeric antigen receptor T cell including the PSMA-targeting single-strand antibody. The PSMA-targeting chimeric antigen receptor can specifically target PSMA positive tumor cells and activate T cells to exert cellular immunity effects to realize efficient and specific killing on the PSMA positive tumor cells, has persistent cell viability and lethality, and cannot damage normal cells. The inventionfurther provides a preparation method and application of the PSMA-targeting chimeric antigen receptor T cell.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a single-chain antibody targeting PSMA, a chimeric antigen receptor T cell, and a preparation method and application thereof. Background technique [0002] Prostate cancer is the most common malignant tumor of the male reproductive system. In the United States, the incidence of prostate cancer ranks first among all malignant tumors, and the mortality rate ranks second, second only to lung cancer. Although the incidence of prostate cancer in my country is far lower than that in western countries, it has shown a significant increase in recent years. Prostate specific membrane antigen (PSMA) is a newly discovered prostate cancer-associated antigen, which has strong prostate tissue specificity and has become an important target protein in the diagnosis and treatment of prostate cancer. There are not many PSMAs on normal prostate cells, but when the cells become cancerous, grow faster ...

Claims

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Application Information

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IPC IPC(8): C07K16/30C12N5/10C12N15/867A61K35/17A61P35/00
CPCA61K35/17A61P35/00C07K14/7051C07K16/3069C07K2317/622C07K2319/02C07K2319/03C07K2319/33C12N15/86C12N2510/02C12N2740/15043
Inventor 万晓春唐超刘绿艳李欣
Owner SHENZHEN BINDEBIOTECH CO LTD
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