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Application of miR-21 in diagnosis and treatment of subarachnoid hemorrhage

A subarachnoid, mir-21 technology, used in medical preparations containing active ingredients, blood diseases, gene therapy, etc. not clear, etc.

Pending Publication Date: 2020-01-14
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the inventors found that mesenchymal stem cell therapy has not been studied much in SAH, and the role of mesenchymal stem cell exosomes is also unclear

Method used

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  • Application of miR-21 in diagnosis and treatment of subarachnoid hemorrhage
  • Application of miR-21 in diagnosis and treatment of subarachnoid hemorrhage
  • Application of miR-21 in diagnosis and treatment of subarachnoid hemorrhage

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Experimental program
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Embodiment 1

[0062] Method and steps

[0063] 1. Culture of mesenchymal stem cells

[0064] Bone marrow-derived mesenchymal stem cells were purchased from Saiye Biotech (Suzhou) and cultured in a-MEM medium containing 10% fetal bovine serum. To extract exosomes, when the cell density was close to 60-80%, the cell culture medium was changed to medium without FBS. After continuing to culture for 48 hours, the cell supernatant was collected and centrifuged to separate exosomes. After extraction, exosomes were stored in a -80°C refrigerator.

[0065] 2. Rat SAH model construction

[0066] The intravascular puncture model of SAH was constructed according to conventional methods. Specifically, adult male SD rats of about 300-320 g were anesthetized by intraperitoneal injection of chloral hydrate, and their left common carotid artery and related limbs were anatomically separated, and then 3- 0 A single-strand nylon suture was inserted into the straightened external carotid artery, and then ex...

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Abstract

The invention provides the application of miR-21 in the diagnosis and treatment of subarachnoid hemorrhage, and belongs to the technical field of biomedicine and molecular biology. According to the application of miR-21 in the diagnosis and treatment of subarachnoid hemorrhage, the potential therapeutic effects of mesenchymal stem cell exosomes in SAH are explored, particularly in the effect on cognitive function. It is proved that the mesenchymal stem cell exosomes can reduce early brain damage after SAH and improve the cognitive function. In the mechanism, it is demonstrated by the exosomesrich in miR-21 play an important role in mediating effect on inhibiting neuronal apoptosis. According to the application of miR-21 in the diagnosis and treatment of subarachnoid hemorrhage, it is actually proved that miR-21 in the mesenchymal stem cell exosomes reduces brain damage after SAH by inhibiting the neuronal apoptosis, the neural behavior is improved through a PTEN / Akt pathway, potentialnovel restorative treatment methods and strategies are provided for SAH patients with the risk of cognitive deficits, and thus the good practical application value is obtained.

Description

technical field [0001] The invention belongs to the technical fields of biomedicine and molecular biology, and specifically relates to the application of miR-21 in the diagnosis and treatment of subarachnoid hemorrhage. Background technique [0002] The information disclosed in the Background of the Invention is only intended to increase the understanding of the general background of the invention, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Subarachnoid hemorrhage (SAH) is a life-threatening neurological disease with high mortality and disability. With the advancement of neurosurgery diagnosis and treatment technology, many patients can survive, but it is completely difficult to restore normal cognitive function, and suffer from cognitive impairment, which reduces their quality of life. Accumulating evidence shows that early brain...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883A61K31/7105A61K48/00A61P9/10A61P25/00A61P7/04
CPCA61K31/7105A61P7/04A61P9/10A61P25/00C12Q1/6883C12Q2600/118C12Q2600/178
Inventor 李刚薛皓王贞高校韩敏邓林赵荣荣
Owner SHANDONG UNIV QILU HOSPITAL
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