Method for detecting roxarsone and eight metabolites of roxarsone in animal body

A Roxar arsenic and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of cheap instruments, good reproducibility, and high extraction efficiency

Inactive Publication Date: 2020-01-14
ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Problems solved by technology

For example, the method disclosed in Chinese patent CN 105866231A for determining the content of total arsenic and valence arsenic in biological tissues and

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  • Method for detecting roxarsone and eight metabolites of roxarsone in animal body
  • Method for detecting roxarsone and eight metabolites of roxarsone in animal body
  • Method for detecting roxarsone and eight metabolites of roxarsone in animal body

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Embodiment Construction

[0043] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0044] The method for detecting roxarsarsenic and its eight metabolites in animals of the present invention comprises the following steps:

[0045] (1) Pretreatment experiment: Take the sample to be tested and add methanol aqueous solution as the extraction solvent, mix well and perform microwave-assisted extraction, take the extract, concentrate it by centrifugation to a constant volume, and pass it through a membrane to obtain the sample solution to be tested;

[0046] (2) Determination of roxarsarsenic and its 8 metabolites: using high performance liquid chromatography-hydride generation-atomic fluorescence On-line detection of nine forms of arsenic such as salt, monomethylarsenic acid, dimethylarsenic acid, 3-amino-4-hydroxyphenylarsonic acid, 4-aminophenylarsonic acid, N-acetyl-4-hydroxyphenylarsonic acid ;

[0047] 1) High performance li...

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Abstract

The invention, which belongs to the technical field of food detection, discloses a method for detecting roxarsone and eight metabolites of the roxarsone in an animal body. Roxarsone and eight metabolites of the roxarsone in all tissues of livestock are measured by combining microwave-assisted extraction with high performance liquid chromatography-hydride generation-atomic fluorescence spectrometryjointly. With the disclosed method, the contents of roxarsone and possible eight metabolites of the roxarsone in all tissues of livestock can be effectively separated and measured and thus the content of the arsenic form in each tissue of the livestock can be accurately analyzed, thereby providing a reliable target for supervision on illegal use of the roxarsone in an animal production link and ensuring the animal foodstuff safety. Therefore, the method has the important significance in evaluating human health risks.

Description

technical field [0001] The invention belongs to the technical field of food detection, and specifically relates to a detection method for roxarsensic and its eight metabolites in animals, and more specifically relates to a microwave-assisted extraction combined with high performance liquid chromatography-hydride generation-atomic fluorescence spectroscopy A method for the determination of Roxars arsenic and its 8 metabolites. Background technique [0002] Roxarsarsenic (3-nitro4-hydroxyphenylarsenic acid, ROX) belongs to the organic arsenic preparation, which has been widely used as a growth-promoting agent for pigs and chickens, and has the functions of preventing diseases, promoting growth, improving feed utilization and improving meat Color etc. However, arsenic residues in livestock and poultry animal products are cause for concern. After ROX enters the animal body, it is absorbed, distributed, and metabolized, and then enters the external environment through excrement...

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Application Information

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IPC IPC(8): G01N30/88G01N30/06G01N30/32G01N30/34G01N30/74
CPCG01N30/06G01N30/32G01N30/34G01N30/74G01N30/88G01N2030/062G01N2030/324G01N2030/8809
Inventor 崔颖赵良娟章骅宓捷波
Owner ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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