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A method for detecting the concentration of an enzymatic reaction substrate

A technology of substrate concentration and detection method, applied in the direction of color/spectral characteristic measurement, etc., can solve problems such as inaccurate test results and changes in reaction speed

Active Publication Date: 2022-02-15
河北清溪生物科技有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to the nature of the enzymatic reaction, the change of the enzyme activity will lead to the change of the reaction speed, that is, the change of the enzyme activity has a great influence on the determination of the substrate concentration, resulting in inaccurate test results.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Urine galactose detection method (dual-enzyme detection system)

[0023] The urine galactose detection device includes a sample injection unit, a detection unit, an arithmetic unit and a display unit, wherein the detection unit contains two detection heads, the detection target is the reflected luminosity of a reagent card, and the detection reagent card contains two detection holes. There is a built-in 1 mmol / L galactose concentration-reflectance curve of different galactose oxidase concentrations inside the urine galactose detection device, which is used to detect the enzyme activity of the reagent. According to the influence of the enzyme activity attenuation of galactose oxidase reagent on the test results during the shelf life obtained from the test, a different galactose concentration-reflection luminosity curve was made for every 6% enzyme activity attenuation, and the enzyme activity attenuation 30% reagent failed. Therefore, the detector also has 6 curves of di...

Embodiment 2

[0026] Triglyceride Determination Method (Tri-enzyme Detection System)

[0027] The triglyceride detection device includes a sample injection unit, a detection unit, an arithmetic unit and a display unit, wherein the detection unit contains two detection heads, the detection target is the grayscale of a reagent card, and the detection reagent card contains two detection holes. The triglyceride detection device has a built-in 0.9mmol / L triglyceride concentration-grayscale curve of different lipase concentrations, which is used to determine the lipase concentration on the reagent. The triglyceride measurement equipment is also equipped with a bottle of 0.9mmol / L triglyceride. The linear range of the concentration measured by the triglyceride detection equipment is 0.5-1.4 mmol / L. According to the degree of influence of the enzyme activity attenuation of the lipase reagent on the test results during the shelf life obtained from the test, the reagent will fail when the enzyme act...

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Abstract

The present invention relates to the field of biochemical detection, in particular to a method for detecting the concentration of an enzymatic reaction substrate, comprising the following steps: step 1, adding a substrate solution of known concentration in the detection hole 1 dropwise, and adding the substrate to be tested dropwise in the detection hole 2 solution, the enzyme activity of the reagent contained in detection hole 1 and detection hole 2 is consistent; step 2, set the reaction time, and detect the reflected luminosity or color of the detection hole 1 and detection hole 2 respectively after the set time; step 3, According to the detection result of the detection hole 1 and the built-in different enzyme activity-reaction degree curves, the reagent enzyme activity is obtained; step 4, according to the enzyme activity obtained in the step 3, the built-in different substrate concentration-reaction degree curves are used, according to the described The detection result of the detection hole 2 obtains the concentration of the substrate to be detected. The application of the method for detecting the substrate concentration of the enzymatic reaction of the present invention can reduce or even eliminate the influence of the change of the enzyme activity on the determination of the substrate concentration.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a method for detecting the concentration of an enzymatic reaction substrate. Background technique [0002] The dry chemical enzymatic method is used to determine the substrate concentration, which is easy to operate, takes less time, and is suitable for rapid inspection projects. [0003] In clinical use, we found that the dry chemical reagents of many photoelectric detection equipment, with the increase of storage time (still within the shelf life) or improper storage conditions (temperature changes and humidity changes) and other factors will lead to a decrease in enzyme activity, affecting the detection. As a result, misdiagnosis was caused. [0004] For example, some tool enzymes such as polyphenol oxidase and galactose oxidase that use metal ions as active centers in dry chemical detection have poor stability, and their active centers are susceptible to inactivation by ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/27
CPCG01N21/27
Inventor 徐詹程
Owner 河北清溪生物科技有限公司
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