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42results about How to "Does not affect the experimental results" patented technology

Method for repairing defect rock sample in rock test

ActiveCN104280281AReduced sample size requirementsEliminate Effects and ErrorsPreparing sample for investigationDefoaming AgentsPetrology
The invention relates to a method for repairing a defect rock sample in rock test. The method comprises the steps of selecting an incomplete rock sample and an undersized rock sample which cannot be subjected to the cutting test and cleavage test, and clearing the dust of the rock sample; preparing K39 crystal resin at the temperature of 35 to 45 DEG C (in warm water bath) according to the following weight percent: 0.2 to 0.8 percent of defoaming agent, 0.3 to 0.5 percent of promoter and 0.6 to 1 percent of hardening agent, sequentially adding the promoter, the hardening agent and the defoaming agent after measuring resin according to the requirement at the interval of 20s, and uniformly stirring the resin; pouring the stirred resin into a clean mold which is coated with lubricating oil until the surface of the mold is covered by the resin, arranging the defect rock sample in the step 1 into the mold, finally adding the resin to a preset size, and waiting for the solidification of the resin; removing the mold 1 to 2 hours later after the solidification of the resin, and polishing the outer surface of the demolded rock sample. By adopting the method, mass acquisition and drilling of the rock sample can be reduced, and the utilization rate of the incomplete and irregular rock sample in the drilling and construction process can be increased.
Owner:泰州市海通资产管理有限公司

Acrosome reaction kit, method for inducing acrosome reaction and detection and method for evaluating acrosome reaction condition

The invention discloses an acrosome reaction kit, which comprises tissue slide preserving fluid, a fluorescent conjugate and a fluorescent sealing solid liquid, wherein each 100 ml of the tissue slide preserving fluid contains 0.5ml of glutaraldehyde, 5ml of methanol, 2ml of ethanol, 0.2g of polyvinylpyrrolidone (PVP), 1ml of formalin and the balance of purified water. The kit can carry out a sperm-induced acrosome reaction and detection; and if calcium ion induction is not carried out, the kit can carry out the acrosome reaction condition evaluation and the acrosome integrity determination. By the tissue slide preserving fluid, the test process for the sperm-induced acrosome reaction and detection is carried out by two parts, samples and test results which are obtained in the first part can be preserved and the final test result cannot be influenced if the operations of the second part are carried out in the subsequent 72 hours, so that the work intensity can be relieved. The fluorescence of a fluorescent staining system is strong and cannot be attenuated after being stabilized for 15 days, so that the detection result is more stable and is easy to interprete and the repeatability of the result can be ensured. The kit and a determination method can be clinically applied and popularized.
Owner:BRED LIFE SCI TECH

Hydroxyl group/keto group synchronous derivatization method of steroid environment endocrine disturbing chemicals

InactiveCN101942006AImplementing Synchronous DerivatizationReduce polarityComponent separationSteroids preparationEstriolLinear correlation
The invention discloses a steroid environment endocrine disturbing chemicals hydroxyl group / keto group synchronous derivatization method of steroid environment endocrine disturbing chemicals (oestrone, 17 beta-estradiol, 17 alpha-ethinyl estradiol, estriol and progesterone). The method comprises the following steps: first fetching standard mixed solution containing the steroid environment endocrine disturbing chemicals and internal standard and introducing high-purity nitrogen at normal temperature so as to dry the standard mixed solution; and adding derivatization reagent prepared from N-methyl-N-trimethylsilyl trifluoroacetamide, iodotrimethylsilane and dithiothreitol based on a proportion of 1,000 muL:5 to 10 muL:5 mg and reacting at 20 to 60 DEG C for 5 to 10 min so as to obtain the derivatization product shown in figure. The method has the advantages of simple and time-saving operation, low energy consumption and cost and the like. The hydroxyl group and the keto group can be derivatized synchronously, the linear correlation among the derivatization products is good, and the detection limit of instrument can reach 0.01 to 1 pg / mu L. The method can be used for the GC-MS detection of the steroid environment endocrine disturbing chemicals in samples such as water, bottom sediment, organisms and the like.
Owner:KUNMING UNIV OF SCI & TECH

Method for measuring migration loss of plasticizer in high impact PVC (polyvinyl chloride) pipe material

The invention relates to a method for measuring migration loss of a plasticizer in a high impact PVC (polyvinyl chloride) pipe material. The method comprises the following steps: (1) blanking three sample strips on the PVC pipe material to be measured through an all-purpose sampling machine, accurately weighing the initial weights of the three sample strips, and respectively recording the initial weights to be m1, m2 and m3; (2) putting each sample strip between two active carbon adsorption sheets, aligning the axis center of each sample strip to the axis centers of the active carbon adsorption sheets to obtain three samples forming an interlayer structure; (3) sequentially and horizontally putting the three samples between two glass sheets, putting a counterweight with the weight of 5Kg on the glass sheets; (4) putting the accumulated glass sheets which are obtained in the step (3) and used for testing into a xenon lamp aging box; (5) starting the xenon lamp aging box and adjusting the temperature of the aging box to be 70 DEG C and starting experiment, after 24h, taking out the sample strips from the xenon lamp aging box, accurately weighing the final weights of the sample strips, and respectively recording the final weights to be m1', m2' and m3'; (6) calculating the weight loss so as to obtain the migration rate of the plasticizer. The method is simple and practical, and high in accuracy.
Owner:GANSU DAYU WATER SAVING

Method for calculating volume of internal void of microcapsule based on solute volatilization system

The invention relates to a method for calculating the volume of an internal void of a microcapsule based on a solute volatilization system. According to the method, the microcapsule is placed in iodine water; an iodine elementary substance not only is volatilized but also enters the microcapsule, and goes in and out of a capsule membrane all the time, so that the inside and outside solubility of the capsule can be balanced; and compared with iodine water in which the microcapsule is not placed, the concentration difference exists. According to the mass conservation and the concentration difference, the volume of the internal void of the microcapsule can be calculated. Compared with a traditional drying method, the method for calculating the internal void of the microcapsule has the advantages that the structure property of the microcapsule is not destroyed, and the time is saved.
Owner:FUZHOU UNIVERSITY

Large-space full-size fire scene simulation experiment control system and method thereof

The invention relates to a large-space full-size fire scene simulation experiment control system and a method thereof. The system is technically characterized by comprising a simulated fire source andconvection fuming system, a temperature data acquisition and processing system, a wind speed data acquisition and processing system, a height marking and image acquisition system, a mobile power supply and a control host which are arranged in a high and large space building; the mobile power supply is connected with the simulated fire source and convection fuming system, the temperature data acquisition and processing system, the wind speed data acquisition and processing system and the height marking and image acquisition system are connected and used for supplying power for the above system; a data acquisition sub-module, a data storage sub-module and a UI display sub-module are arranged in the control host to realize the functions of processing and controlling the field data. The system disclosed by the invention adopts a 1-WIRE bus mode to perform networking on the sensors, and has the characteristics of being simple and convenient for wiring and flexible in mounting and dismounting, and strong in stability of data transmission; and the problem that the air inlet at one side interferes with hot smoke by adopting a fume generating device with air inlet at two sides is avoided.
Owner:应急管理部天津消防研究所

A method for detecting the concentration of an enzymatic reaction substrate

The present invention relates to the field of biochemical detection, in particular to a method for detecting the concentration of an enzymatic reaction substrate, comprising the following steps: step 1, adding a substrate solution of known concentration in the detection hole 1 dropwise, and adding the substrate to be tested dropwise in the detection hole 2 solution, the enzyme activity of the reagent contained in detection hole 1 and detection hole 2 is consistent; step 2, set the reaction time, and detect the reflected luminosity or color of the detection hole 1 and detection hole 2 respectively after the set time; step 3, According to the detection result of the detection hole 1 and the built-in different enzyme activity-reaction degree curves, the reagent enzyme activity is obtained; step 4, according to the enzyme activity obtained in the step 3, the built-in different substrate concentration-reaction degree curves are used, according to the described The detection result of the detection hole 2 obtains the concentration of the substrate to be detected. The application of the method for detecting the substrate concentration of the enzymatic reaction of the present invention can reduce or even eliminate the influence of the change of the enzyme activity on the determination of the substrate concentration.
Owner:河北清溪生物科技有限公司

Efficient akebia trifoliate ploidy detection method

The invention discloses an efficient akebia trifoliate ploidy detection method. The method comprises the steps of preparing a sample pretreatment agent; to-be-detected material sampling: selecting materials such as calluses, hypocotyls, cotyledons, suspension culture cells, leaves and the like, repeatedly washing and cleaning by using sterilized double distilled water when the experimental materials are taken, selecting tender parts, taking 480 to 510mg of samples, and avoiding tissues such as plant veins, vascular bundles and the like with high metabolite content during sampling; preparing a cell nucleus suspension; performing instrument detection and quality control of a corresponding fluorescence channel; performing contrast and standard flow cytometry detection; and performing data comparison and ploidy determination. The method has the advantages that pretreatment steps are simplified, the cell nucleus suspension required by detection is efficiently prepared, RNA interference is eliminated, flow cytometry sample loading parameters are standardized, identification results are unified and standard, multiple methods are combined to guarantee accurate results, large-scale detection is achieved, results are visualized, and data transmission is convenient and fast.
Owner:SICHUAN AGRI UNIV

Interactive training platform based on real PLC and computer virtual elevator components

An interactive training platform based on real PLC and computer virtual elevator components, including PLC, computer, single-chip microcomputer signal conversion transceiver module, training junction box; the computer is installed with PLC program development software, and virtual control training component units, virtual Elevator installation unit, virtual elevator running diagram unit software; the platform edits and runs the elevator program on the real PLC, and other elevator components are virtualized on the computer. The virtual components have the same position, status, and connection properties with the PLC terminals as the real ones; single-chip microcomputer signals The conversion transceiver module is connected with the computer and the PLC, so that the PLC terminal signal is synchronized with the status of the virtual elevator components; the training junction box has connection terminals, which correspond to the control terminals of the virtual elevator components, and can be used for PLC control wiring training. The platform can carry out PLC programming, and use virtual elevator components to simulate elevator operation, and complete various scheduled training projects. It has the advantages of small equipment investment and small equipment footprint.
Owner:JIAOZUO UNIV

Hydroxyl group/keto group synchronous derivatization method of endocrine disturbing chemicals in steroid environment

InactiveCN101942006BImplementing Synchronous DerivatizationReduce polarityComponent separationSteroids preparationEstriolLinear correlation
The invention discloses a steroid environment endocrine disturbing chemicals hydroxyl group / keto group synchronous derivatization method of steroid environment endocrine disturbing chemicals (oestrone, 17 beta-estradiol, 17 alpha-ethinyl estradiol, estriol and progesterone). The method comprises the following steps: first fetching standard mixed solution containing the steroid environment endocrine disturbing chemicals and internal standard and introducing high-purity nitrogen at normal temperature so as to dry the standard mixed solution; and adding derivatization reagent prepared from N-methyl-N-trimethylsilyl trifluoroacetamide, iodotrimethylsilane and dithiothreitol based on a proportion of 1,000 muL:5 to 10 muL:5 mg and reacting at 20 to 60 DEG C for 5 to 10 min so as to obtain the derivatization product shown in figure. The method has the advantages of simple and time-saving operation, low energy consumption and cost and the like. The hydroxyl group and the keto group can be derivatized synchronously, the linear correlation among the derivatization products is good, and the detection limit of instrument can reach 0.01 to 1 pg / mu L. The method can be used for the GC-MS detection of the steroid environment endocrine disturbing chemicals in samples such as water, bottom sediment, organisms and the like.
Owner:KUNMING UNIV OF SCI & TECH
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