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42results about How to "Does not affect the experimental results" patented technology

Reagent for processing trace whole blood and application thereof

The invention relates to a reagent for processing trace whole blood and an application thereof. The reagent comprises the following components: a buffer solution, an osmotic pressure retaining agent, a protective agent, an anticoagulant and a preservative. By adopting the reagent, a trace whole blood sample can be diluted by optional times (2-1000 times) and treated to obtain dilution of serum or plasma without damaging the sample and causing erythrocyte hematolysis.
Owner:BEIJING BOHUI INNOVATION TECH

Print domain trademark anti-counterfeit method based on digital watermark technology

The invention relates to a print field mark false-proof method, based on digit watermark technique, wherein the mark image is embedded with watermark false-proof information, which comprises: 1, using self-adaptable watermark strength to embed two-dimension random copy watermark into the idle field of original mark image, to generate false-proof mark image; 2, using false-proof mark image and one-dimension Ladon transformation to generate self-registration template, to be used to calculate self-registration geometrical deformation parameter at the mark false check step; 3, generating two-dimension random copy watermark which is same as in step 1; the image collector collects tested mark image, extracting self-registration parameter; based on self-registration parameter, synchronizing the two-dimension random copy watermark; based on synchronized watermark and tested mark image, using self-related judgment, to realize the recognition of real and false mark.
Owner:BEIJING JIAOTONG UNIV

Method for repairing defect rock sample in rock test

ActiveCN104280281AReduced sample size requirementsEliminate Effects and ErrorsPreparing sample for investigationDefoaming AgentsPetrology
The invention relates to a method for repairing a defect rock sample in rock test. The method comprises the steps of selecting an incomplete rock sample and an undersized rock sample which cannot be subjected to the cutting test and cleavage test, and clearing the dust of the rock sample; preparing K39 crystal resin at the temperature of 35 to 45 DEG C (in warm water bath) according to the following weight percent: 0.2 to 0.8 percent of defoaming agent, 0.3 to 0.5 percent of promoter and 0.6 to 1 percent of hardening agent, sequentially adding the promoter, the hardening agent and the defoaming agent after measuring resin according to the requirement at the interval of 20s, and uniformly stirring the resin; pouring the stirred resin into a clean mold which is coated with lubricating oil until the surface of the mold is covered by the resin, arranging the defect rock sample in the step 1 into the mold, finally adding the resin to a preset size, and waiting for the solidification of the resin; removing the mold 1 to 2 hours later after the solidification of the resin, and polishing the outer surface of the demolded rock sample. By adopting the method, mass acquisition and drilling of the rock sample can be reduced, and the utilization rate of the incomplete and irregular rock sample in the drilling and construction process can be increased.
Owner:泰州市海通资产管理有限公司

Fracture end protective agent used for brittle fiber mechanics measurement, its proparation and use

A method for preparing protectant of brittle fiber breaking end in mechanical test includes using white oil, water, wetting agent and emulsifier as main composition then carrying out water phase preparation, oil phase preparation and two phase mixed emulsification on water ¿C in- oil latex for obtaining protectant of breaking end.
Owner:DONGHUA UNIV

Special feed for laboratory mice and preparation method thereof

InactiveCN105685426AMeet the nutritional needs of growth and developmentKeep healthyAnimal feeding stuffLaboratory mouseSaccharum
The present invention discloses a special feed for laboratory mice and a preparation method thereof, and relates to the technical field of laboratory animal feed. The special feed for experiment mice is composed of the following raw materials by weight: 30-40 parts of corn, 20-40 parts of flour, 20-40 parts of rice, 20-40 parts of soybean, 10-20 parts of fish meal, 5-8 parts of elderberry powder, 3-5 parts of eggshell powder, 1-3 parts of lotus powder, 3-5 parts of sucrose, 1-3 parts of fructose, 1-3 parts of vegetable oil, 1-3 parts of lard, 0.1-0.3 parts of a mineral salt premix, 0.1-0.3 part of an amino acid premix, 0.1-0.2 part of a vitamin premix, and 0.1-0.2 part of a trace element premix. The feed employs high quality ingredients in reasonable ratio, not only meets the nutritional needs of growth and development of laboratory mice but also is free of heavy metals and pesticide residues, so as to ensure the health of the mice, and avoid affects on experimental results.
Owner:GUANGXI UNIV

Acrosome reaction kit, method for inducing acrosome reaction and detection and method for evaluating acrosome reaction condition

The invention discloses an acrosome reaction kit, which comprises tissue slide preserving fluid, a fluorescent conjugate and a fluorescent sealing solid liquid, wherein each 100 ml of the tissue slide preserving fluid contains 0.5ml of glutaraldehyde, 5ml of methanol, 2ml of ethanol, 0.2g of polyvinylpyrrolidone (PVP), 1ml of formalin and the balance of purified water. The kit can carry out a sperm-induced acrosome reaction and detection; and if calcium ion induction is not carried out, the kit can carry out the acrosome reaction condition evaluation and the acrosome integrity determination. By the tissue slide preserving fluid, the test process for the sperm-induced acrosome reaction and detection is carried out by two parts, samples and test results which are obtained in the first part can be preserved and the final test result cannot be influenced if the operations of the second part are carried out in the subsequent 72 hours, so that the work intensity can be relieved. The fluorescence of a fluorescent staining system is strong and cannot be attenuated after being stabilized for 15 days, so that the detection result is more stable and is easy to interprete and the repeatability of the result can be ensured. The kit and a determination method can be clinically applied and popularized.
Owner:BRED LIFE SCI TECH

Hydroxyl group/keto group synchronous derivatization method of steroid environment endocrine disturbing chemicals

InactiveCN101942006AImplementing Synchronous DerivatizationReduce polarityComponent separationSteroids preparationEstriolLinear correlation
The invention discloses a steroid environment endocrine disturbing chemicals hydroxyl group / keto group synchronous derivatization method of steroid environment endocrine disturbing chemicals (oestrone, 17 beta-estradiol, 17 alpha-ethinyl estradiol, estriol and progesterone). The method comprises the following steps: first fetching standard mixed solution containing the steroid environment endocrine disturbing chemicals and internal standard and introducing high-purity nitrogen at normal temperature so as to dry the standard mixed solution; and adding derivatization reagent prepared from N-methyl-N-trimethylsilyl trifluoroacetamide, iodotrimethylsilane and dithiothreitol based on a proportion of 1,000 muL:5 to 10 muL:5 mg and reacting at 20 to 60 DEG C for 5 to 10 min so as to obtain the derivatization product shown in figure. The method has the advantages of simple and time-saving operation, low energy consumption and cost and the like. The hydroxyl group and the keto group can be derivatized synchronously, the linear correlation among the derivatization products is good, and the detection limit of instrument can reach 0.01 to 1 pg / mu L. The method can be used for the GC-MS detection of the steroid environment endocrine disturbing chemicals in samples such as water, bottom sediment, organisms and the like.
Owner:KUNMING UNIV OF SCI & TECH

Representation method of membrane pore diameter of microporous membrane

The invention relates to a representation method of membrane pore diameter of a microporous membrane. The representation method comprises the following steps: (1) artificially preparing spherical SiO2microspheres with normal particle-size distribution and preparing into 10wt% suspension; (2) adopting a microporous membrane to filter the suspension and simulating raw-water experiment; (3) adoptinga laser particle-size analyzer to test distribution of particles in filtrate before and after filtering; (4) carrying out fitting conversion on particle-size distribution data of filtered SiO2; (5) finally carrying out drying treatment on the filtrate, and assisting representation by powder mass change before and after filtering. Different from the conventional bubble-point method, mercury intrusion method and electron-microscope photographing method, the representation method has the advantages of being closer to actual use-performance evaluation, low in cost of used consumables, good to environments and testers, simple and fast in testing speed and vivid and scientific in experimental result, is very suitable for test of large-batch various membranes, and is beneficial to promotion of membrane products.
Owner:HUZHOU COLLEGE

Improved fume hood

The invention discloses an improved fume hood, belongs to the field of experimental equipment and aims at solving the technical problem that toxic substances produced by the traditional chemical experimental table harm the safety of experimental personnel. According to the technical scheme of the invention, the improved fume hood comprises a left side plate, a right side plate, a baffle and a workbench, wherein the left side plate and the right side plate are installed at the left and right ends of the workbench respectively; the baffle is installed between the left side plate and the right side plate; a glass door and a diversion plate are arranged on the front and back sides of the baffle respectively; the left side plate, the right side plate, the baffle, the workbench, the glass door and the diversion plate enclose a cavity; the gas suction end of an exhaust blower extends into the cavity; the exhaust end of the exhaust blower extends out of the cavity; an air outlet of an air blower is formed in the cavity; and an air inlet of the air blower is formed outsides the cavity. The improved fume hood has the beneficial effects that interference on vision of the experimental personnel is reduced, the personal safety of the experimental personnel is ensured, and an experimental result is not influenced.
Owner:江苏库利南实验室系统工程有限公司

Method for measuring migration loss of plasticizer in high impact PVC (polyvinyl chloride) pipe material

The invention relates to a method for measuring migration loss of a plasticizer in a high impact PVC (polyvinyl chloride) pipe material. The method comprises the following steps: (1) blanking three sample strips on the PVC pipe material to be measured through an all-purpose sampling machine, accurately weighing the initial weights of the three sample strips, and respectively recording the initial weights to be m1, m2 and m3; (2) putting each sample strip between two active carbon adsorption sheets, aligning the axis center of each sample strip to the axis centers of the active carbon adsorption sheets to obtain three samples forming an interlayer structure; (3) sequentially and horizontally putting the three samples between two glass sheets, putting a counterweight with the weight of 5Kg on the glass sheets; (4) putting the accumulated glass sheets which are obtained in the step (3) and used for testing into a xenon lamp aging box; (5) starting the xenon lamp aging box and adjusting the temperature of the aging box to be 70 DEG C and starting experiment, after 24h, taking out the sample strips from the xenon lamp aging box, accurately weighing the final weights of the sample strips, and respectively recording the final weights to be m1', m2' and m3'; (6) calculating the weight loss so as to obtain the migration rate of the plasticizer. The method is simple and practical, and high in accuracy.
Owner:GANSU DAYU WATER SAVING

Method for calculating volume of internal void of microcapsule based on solute volatilization system

The invention relates to a method for calculating the volume of an internal void of a microcapsule based on a solute volatilization system. According to the method, the microcapsule is placed in iodine water; an iodine elementary substance not only is volatilized but also enters the microcapsule, and goes in and out of a capsule membrane all the time, so that the inside and outside solubility of the capsule can be balanced; and compared with iodine water in which the microcapsule is not placed, the concentration difference exists. According to the mass conservation and the concentration difference, the volume of the internal void of the microcapsule can be calculated. Compared with a traditional drying method, the method for calculating the internal void of the microcapsule has the advantages that the structure property of the microcapsule is not destroyed, and the time is saved.
Owner:FUZHOU UNIVERSITY

Large-space full-size fire scene simulation experiment control system and method thereof

The invention relates to a large-space full-size fire scene simulation experiment control system and a method thereof. The system is technically characterized by comprising a simulated fire source andconvection fuming system, a temperature data acquisition and processing system, a wind speed data acquisition and processing system, a height marking and image acquisition system, a mobile power supply and a control host which are arranged in a high and large space building; the mobile power supply is connected with the simulated fire source and convection fuming system, the temperature data acquisition and processing system, the wind speed data acquisition and processing system and the height marking and image acquisition system are connected and used for supplying power for the above system; a data acquisition sub-module, a data storage sub-module and a UI display sub-module are arranged in the control host to realize the functions of processing and controlling the field data. The system disclosed by the invention adopts a 1-WIRE bus mode to perform networking on the sensors, and has the characteristics of being simple and convenient for wiring and flexible in mounting and dismounting, and strong in stability of data transmission; and the problem that the air inlet at one side interferes with hot smoke by adopting a fume generating device with air inlet at two sides is avoided.
Owner:应急管理部天津消防研究所

High-amplitude-phase-consistency teaching experiment module separating circuit

The invention discloses a high-amplitude-phase-consistency teaching experiment module separating circuit, and the circuit consists of at least two separating channels. Each separating channel comprises an experiment input switch, an experiment input circuit, a module circuit separating switch, an experiment output circuit, and an experiment output switch. According to the invention, the circuit can enable each module unit circuit which needs to be used in the development of a teaching experiment to be separated from a signal link, enables the input and output ends of the each module unit circuit to be led to an experiment input interface and an experiment output interface, and forms a teaching experiment module which can select the switching and carry out the experiment independently. The high-amplitude-phase-consistency design enables all circuits of the teaching experiment module separating circuit to have the same electric length and amplitude-phase properties in an allowed error range, so as to correct the insertion loss and phase errors introduced by a separating circuit through a correction method.
Owner:THE 41ST INST OF CHINA ELECTRONICS TECH GRP

Determination method of longicorn larva epidermis hydrocarbon and application of determination method

The invention discloses a determination method of longicorn larva epidermis hydrocarbon, the determination method comprises the following steps: step 1, cleaning the surface of longicorn larva clearly; step 2, performing freeze drying; step 3, extracting a sample, wherein the extracting time is 10 to 30min; step 4, concentrating extraction liquid; step 5, performing GC-MS analysis; step 6, performing data analysis on obtained results of the GC-MS analysis, the data analysis comprises qualitative analysis and quantitative analysis. Moreover, the invention also discloses application of the determination method in classification and identification methods and systems of the longicorn larva. The determination method disclosed by the invention has the advantages of easy operation, low cost, short experiment period, thus the human and material resources are greatly saved, the quarantine period is shortened, and the determination method solves the difficult problem that the classification and determination technique is difficult to identify the longicorn larva with hard-to-identified forms.
Owner:PLANTS & ANIMALS & FOOD TESTING QUARANTINE TECH CENT SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Crucible cleaning equipment

The invention discloses crucible cleaning equipment which comprises a frame and a moving platform installed on the frame. A clamping mechanism used for clamping a crucible is installed on the moving platform; a blowing and sweeping platform and a brushing and sweeping platform which are sequentially arranged in the moving direction are arranged on the side face of the moving platform; an air nozzle is installed on the blowing and sweeping platform; a brush is installed on the brushing and sweeping platform; and the clamping mechanism can vertically move along the moving platform, and enables the opening of the clamped crucible to be aligned with the brush and the air nozzle in sequence. In the working process, a crucible to be cleaned is placed on the clamping mechanism, clamped through the clamping mechanism, conveyed to the brushing and sweeping platform, brushed and swept through the brush, then conveyed to the blowing and sweeping platform, blown and swept through the air nozzle, and finally conveyed out through the clamping mechanism. Automatic cleaning is achieved, the working efficiency is improved, manual intervention is not needed, no harm is caused to people, cleaning is thorough, and the next experiment result is not affected.
Owner:长沙开元仪器有限公司

A method for characterizing the pore size of microporous membranes

The invention relates to a method for characterizing the pore size of a microporous membrane. The method includes the following steps: (1) Artificially modulating spherical SiO with a normal particle size distribution 2 Microspheres are configured into 10wt% suspension; (2) microporous membrane filters this suspension to simulate the raw water experiment; (3) adopts a laser particle size analyzer to test the particle distribution in the filtrate before and after filtration; (4) Filtered SiO 2 The particle size distribution data is fitted and converted; (5) Finally, the filtrate is dried, and the powder mass change before and after filtration is used as an auxiliary characterization. The present invention is different from the conventional bubble point method, mercury intrusion method and electron microscope photo method, and has the advantages of being closer to the actual use performance evaluation, low cost of consumables, good for the environment and testers, simple and fast test speed, and scientific image of the experimental results. It is very suitable for large-scale and multi-type membrane testing, and is conducive to the promotion of membrane products.
Owner:HUZHOU COLLEGE

Falling device cleaning mechanism

The invention discloses a falling device cleaning mechanism. The mechanism comprises a fixing plate, wherein a clamping device used for clamping to fix a falling device and a rotating device used for driving the clamping device to rotate are arranged on the fixing plate. the fixing plate is provided with an upper cleaning device for blowing high-pressure airflow to the discharge port of the falling device and a first telescopic device for driving the upper cleaning device to move relative to the clamping device above the clamping device, and the fixing plate is provided with a lower cleaning device for blowing high-pressure airflow to the feeding port of the falling device and a second telescopic device for driving the lower cleaning device to move relative to the clamping device below the clamping device. The high-pressure airflow is blown into the discharging port from top to bottom through the upper cleaning device, then the high-pressure air is blown into the feeding port of the falling device from bottom to top through the lower cleaning device, it is guaranteed that the falling device is thoroughly cleaned, and the next experiment result is not affected. Automatic cleaning is achieved, the working efficiency is improved, manual intervention is not needed, and no harm is caused to people.
Owner:长沙开元仪器有限公司

Method for removing dressing at tab part of pole piece for experiment

The invention discloses a method for removing a dressing at a tab part of a pole piece for an experiment. The method specifically comprises the following steps of a preparation operation step, a stripping operation step and a post-treatment operation step. The stripping operation comprises the following steps that a, a container is placed on one side of an operation table top, hot water at the temperature of 60-80 DEG C is poured into the container, and the distance between the liquid level of the hot water and the upper edge of the container is 2-3 cm; b, the tab of the pole piece is placed at the lower part, the tab of the pole piece is slowly put into hot water to a specified position, stays for 15-20 seconds and then is taken out; c, the dressing layer on the tab of the pole piece treated in the step b isscraped off by using a plastic hard board; and d, the tab of the pole piece treated in the step c iswiped by using a rag, toilet paper is dipped in a small amount of alcohol, and the two sides of the tab arewiped cleanly. The method can be used for removing the dressing carried by the tab part of the pole piece due to special reasons, and is simple in removal method and suitable for being used in laboratories.
Owner:潍坊聚能电池有限公司

Rolling type multi-gear cigarette sorting device and cigarette sorting method

PendingCN113828551AIndependent of each otherNo human assistanceSortingStructural engineeringMechanical engineering
The invention provides a rolling type multi-gear cigarette sorting device. The device comprises a cigarette picker (1), a screening panel (2), a partition panel (3) and a storage box (4), the storage box (4) comprises N material receiving boxes (41), and each material receiving box (41) corresponds to an independent screening channel which does not affect each other. The cigarette picker (1) is connected with negative pressure equipment so as to suck cigarettes from the previous process and cut off the negative pressure equipment, and the cigarettes sucked by the cigarette picker (1) fall into the corresponding material receiving boxes (41) through independent screening channel openings. A plurality of screening channel baffles (21) are arranged on a screening panel (2) of the sorting device, a single screening channel baffle (21) is opened to form the screening channel connected to the material receiving boxes (41), the screening channel baffle (21) is closed to close the screening channel, so that a plurality of the screening channels are not mutually influenced, and samples with high similarity are grouped and put into the specified material receiving box (41).
Owner:CHINA TOBACCO YUNNAN IND

Method and device of acquiring experiment design table of artificial microstructure

ActiveCN103136403AFew parameters to be testedThe number of trials does not affectElectrical testingSpecial data processing applicationsUniform designAlgorithm
The invention discloses a method and a device of acquiring an experiment design table of an artificial microstructure. The method comprises confirming the number of geometrical parameter factors of an artificial microstructure to be tested, the number of values of each geometrical parameter factor and the parameter values corresponding to each geometrical parameter factor, choosing a left-circulating Latin square uniform design method to build a uniform design table according to the number of the geometrical parameter factors and the number of the values of each geometrical parameter factor, and building and obtaining the experiment design table according to the confirmed values of each geometrical parameter factor and the uniform design table. The method and the device of acquiring the experiment design table of the artificial microstructure can reduce the times of experiments under the condition that an experiment result is not influenced, and the undistorted experiment result is obtained in the experiments of a small number.
Owner:KUANG CHI INST OF ADVANCED TECH

A method for detecting the concentration of an enzymatic reaction substrate

The present invention relates to the field of biochemical detection, in particular to a method for detecting the concentration of an enzymatic reaction substrate, comprising the following steps: step 1, adding a substrate solution of known concentration in the detection hole 1 dropwise, and adding the substrate to be tested dropwise in the detection hole 2 solution, the enzyme activity of the reagent contained in detection hole 1 and detection hole 2 is consistent; step 2, set the reaction time, and detect the reflected luminosity or color of the detection hole 1 and detection hole 2 respectively after the set time; step 3, According to the detection result of the detection hole 1 and the built-in different enzyme activity-reaction degree curves, the reagent enzyme activity is obtained; step 4, according to the enzyme activity obtained in the step 3, the built-in different substrate concentration-reaction degree curves are used, according to the described The detection result of the detection hole 2 obtains the concentration of the substrate to be detected. The application of the method for detecting the substrate concentration of the enzymatic reaction of the present invention can reduce or even eliminate the influence of the change of the enzyme activity on the determination of the substrate concentration.
Owner:河北清溪生物科技有限公司

Clamp-type immunoblotting analyzer and control system thereof

The invention provides a clamp-type immunoblotting analyzer and a control system thereof. The clamp-type immunoblotting analyzer comprises a pedestal, a sample film clamping piece, a lofting transferarea, a reaction temperature area, and a transfer unit. The transfer unit comprises a chuck and an XYZ three-axis moving mechanism. The chuck is provided with a mechanism for carrying out taking and placing of the sample film clamping piece. The XYZ three-axis moving mechanism drives the chuck to move between the lofting transfer area and each reaction temperature area. The invention also providesa reagent storage area so that an expensive reagent can be conveniently recycled and experiment cost is reduced. A new sample film can be inserted into the immunoblotting analyzer to carry out reaction at any time, and test steps and conditions of each sample film can be separately and individually set, which is suitable for test operation of laboratory personnel. Usage efficiency of the immunoblotting analyzer is greatly improved, waiting time for queuing is reduced, and a test speed is increased.
Owner:MONAD SUZHOU BIOTECH CO LTD

An efficient air conditioning performance debugging method and system

The invention provides an efficient air conditioner performance debugging method and system, and relates to the technical field of air conditioners. The debugging system comprises a condenser, a compressor, a throttling device and an evaporator; the debugging system is characterized in that a first part is detachably connected to a first end of the condenser, and a second part is detachably connected to a second end of the condenser; a first end of the compressor is provided with a first connecting piece matched with the first part; a first end of the throttling device is provided with a second connecting piece matched with the second part; the first part is connected with the first connecting piece; and the second part is connected with the second connecting piece. By improving an outdoorunit of an air conditioner, the condenser can be connected in the system in a detachable manner so that a set of prototype can debug different condenser schemes.
Owner:NINGBO AUX ELECTRIC +1

Tissue chip receptor blank wax block preparation instrument

The invention provides a tissue chip receptor blank wax block preparation instrument, which comprises an upper cover (100), a sliding block (200) and a supporting base (300), wherein a first guide hole (301) is formed in the supporting base (300), the first guide hole (301) is provided with a demolding block (304) moving along the first guide hole (301), a second guide hole (302) is formed in thesupporting base (300), a vertical rod (103) moves up and down along the second guide hole (302), the vertical rod (103) is fixed to a top plate (104), and the upper surface of the top plate (104) canabut against the lower surface of the demolding block (304). Due to the fact that array columns (202) are transversely arranged rather than longitudinally arranged, a wax liquid can be attached to theside surface of the sliding block (200) to form a flat plane, thus animal and plant tissue chips and agentia can be conveniently arranged in concave holes of wax blocks, experiment operation is facilitated, and experiment results are not affected.
Owner:赵慧

Efficient akebia trifoliate ploidy detection method

The invention discloses an efficient akebia trifoliate ploidy detection method. The method comprises the steps of preparing a sample pretreatment agent; to-be-detected material sampling: selecting materials such as calluses, hypocotyls, cotyledons, suspension culture cells, leaves and the like, repeatedly washing and cleaning by using sterilized double distilled water when the experimental materials are taken, selecting tender parts, taking 480 to 510mg of samples, and avoiding tissues such as plant veins, vascular bundles and the like with high metabolite content during sampling; preparing a cell nucleus suspension; performing instrument detection and quality control of a corresponding fluorescence channel; performing contrast and standard flow cytometry detection; and performing data comparison and ploidy determination. The method has the advantages that pretreatment steps are simplified, the cell nucleus suspension required by detection is efficiently prepared, RNA interference is eliminated, flow cytometry sample loading parameters are standardized, identification results are unified and standard, multiple methods are combined to guarantee accurate results, large-scale detection is achieved, results are visualized, and data transmission is convenient and fast.
Owner:SICHUAN AGRI UNIV

Photoelectric detection equipment and detection method thereof

The invention relates to the field of biochemical detection, in particular to photoelectric detection equipment and a detection method thereof. The photoelectric detection equipment comprises a sampleinjection unit, a detection unit, an operation unit and a display unit, wherein the detection unit comprises a photoelectric detector with two detection heads and a reagent card with two detection holes; a hole 1 is used for detecting the enzyme activity on a reagent sheet; and the substrate concentration to be measured is detected according to the enzyme activity measured by the hole 1 and the detection result of a hole 2. The enzyme activities of multiple dry chemical reagent sheets decrease along with the increase of storage time (still within the shelf life) or the change of storage conditions, resulting in inaccurate detection results. Through the combined application of the detection equipment and the detection method thereof disclosed by the invention, the influence of the change of the enzyme activity on the reagent sheet on the determination of the substrate concentration can be lowered or even eliminated.
Owner:河北清溪生物科技有限公司

Interactive training platform based on real PLC and computer virtual elevator components

An interactive training platform based on real PLC and computer virtual elevator components, including PLC, computer, single-chip microcomputer signal conversion transceiver module, training junction box; the computer is installed with PLC program development software, and virtual control training component units, virtual Elevator installation unit, virtual elevator running diagram unit software; the platform edits and runs the elevator program on the real PLC, and other elevator components are virtualized on the computer. The virtual components have the same position, status, and connection properties with the PLC terminals as the real ones; single-chip microcomputer signals The conversion transceiver module is connected with the computer and the PLC, so that the PLC terminal signal is synchronized with the status of the virtual elevator components; the training junction box has connection terminals, which correspond to the control terminals of the virtual elevator components, and can be used for PLC control wiring training. The platform can carry out PLC programming, and use virtual elevator components to simulate elevator operation, and complete various scheduled training projects. It has the advantages of small equipment investment and small equipment footprint.
Owner:JIAOZUO UNIV

Hydroxyl group/keto group synchronous derivatization method of endocrine disturbing chemicals in steroid environment

InactiveCN101942006BImplementing Synchronous DerivatizationReduce polarityComponent separationSteroids preparationEstriolLinear correlation
The invention discloses a steroid environment endocrine disturbing chemicals hydroxyl group / keto group synchronous derivatization method of steroid environment endocrine disturbing chemicals (oestrone, 17 beta-estradiol, 17 alpha-ethinyl estradiol, estriol and progesterone). The method comprises the following steps: first fetching standard mixed solution containing the steroid environment endocrine disturbing chemicals and internal standard and introducing high-purity nitrogen at normal temperature so as to dry the standard mixed solution; and adding derivatization reagent prepared from N-methyl-N-trimethylsilyl trifluoroacetamide, iodotrimethylsilane and dithiothreitol based on a proportion of 1,000 muL:5 to 10 muL:5 mg and reacting at 20 to 60 DEG C for 5 to 10 min so as to obtain the derivatization product shown in figure. The method has the advantages of simple and time-saving operation, low energy consumption and cost and the like. The hydroxyl group and the keto group can be derivatized synchronously, the linear correlation among the derivatization products is good, and the detection limit of instrument can reach 0.01 to 1 pg / mu L. The method can be used for the GC-MS detection of the steroid environment endocrine disturbing chemicals in samples such as water, bottom sediment, organisms and the like.
Owner:KUNMING UNIV OF SCI & TECH
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