Hydroxyl group/keto group synchronous derivatization method of steroid environment endocrine disturbing chemicals

A technology based on endocrine disruptors and hydroxyketones, which is applied in the field of environmental analytical chemistry, can solve the problems of large differences in catalyst addition, reduced detection accuracy, increased energy consumption and cost, and saves heating steps and reduces experimental costs. , the effect of reducing energy consumption and cost
CN101942006AInactive Publication Date: 2011-01-12KUNMING UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
KUNMING UNIV OF SCI & TECH
Publication Date
2011-01-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a steroid environment endocrine disturbing chemicals hydroxyl group / keto group synchronous derivatization method of steroid environment endocrine disturbing chemicals (oestrone, 17 beta-estradiol, 17 alpha-ethinyl estradiol, estriol and progesterone). The method comprises the following steps: first fetching standard mixed solution containing the steroid environment endocrine disturbing chemicals and internal standard and introducing high-purity nitrogen at normal temperature so as to dry the standard mixed solution; and adding derivatization reagent prepared from N-methyl-N-trimethylsilyl trifluoroacetamide, iodotrimethylsilane and dithiothreitol based on a proportion of 1,000 muL:5 to 10 muL:5 mg and reacting at 20 to 60 DEG C for 5 to 10 min so as to obtain the derivatization product shown in figure. The method has the advantages of simple and time-saving operation, low energy consumption and cost and the like. The hydroxyl group and the keto group can be derivatized synchronously, the linear correlation among the derivatization products is good, and the detection limit of instrument can reach 0.01 to 1 pg / mu L. The method can be used for the GC-MS detection of the steroid environment endocrine disturbing chemicals in samples such as water, bottom sediment, organisms and the like.
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Description

technical field

[0001] The invention belongs to the field of environmental analytical chemistry, and relates to a method for synchronous derivatization of hydroxyketone groups of steroid environmental endocrine disruptors (estrone, 17β-estradiol, estriol, 17α-ethinyl estradiol and progesterone) , is the sample pretreatment method and core technology used by gas chromatography-mass spectrometry to analyze such substances. Background technique

[0002] Environmental endocrine disrupting chemicals (Endocrine Disrupting Chemicals, EDCs), also known as environmental hormones (Environmental Hormones), refer to natural or artificial emissions in the environment, which can interfere or inhibit the endocrine system by simulating the physiological and biochemical effects in animals or humans. , and a large class of exogenous compounds that affect reproductive, nervous, and immune system functions. EDCs are highly toxic and bioaccumulative, and can interfere with the endocrine system ...

Claims

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