Determination method of longicorn larva epidermis hydrocarbon and application of determination method

A technology of hydrocarbons and beetle larvae, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as classification and identification of long beetle larvae, and achieve the effects of strictly preventing introduction and spread, reducing economic losses, and stabilizing properties

Inactive Publication Date: 2017-05-31
PLANTS & ANIMALS & FOOD TESTING QUARANTINE TECH CENT SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention aims at the difficult morphological identification of longhorn beetle larvae as the research object, by intercepting more spruce longhorn beetle, spruce little longhorn beetle, spruce long beetle longicorn beetle, North American long beetle beetle, and concave beetle at the Shanghai port. Determination and analysis of the skin hydrocarbon components of the larvae of five species of longhorn beetles with damaged wings, established a method and system for the classification and identification of insect skin hydrocarbons that can be applied to the larvae of longhorn beetles, and solved the problem of difficult morphological identification of longhorn beetles. The classification and identification of larvae is a technical problem, and it has broad application prospects in improving the identification ability of longhorn beetle larvae and the detection rate of ports

Method used

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  • Determination method of longicorn larva epidermis hydrocarbon and application of determination method
  • Determination method of longicorn larva epidermis hydrocarbon and application of determination method
  • Determination method of longicorn larva epidermis hydrocarbon and application of determination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Instruments and reagents

[0045] Instruments: gas chromatography and mass spectrometry (Agilent-7890A); freeze dryer (LABCONCO); nitrogen blowing instrument (HGC-12A)

[0046] Reagent: n-hexane (analytical alcohol) (Sinopharm Chemical Reagent Co., Ltd.)

[0047] 2. Sample preparation

[0048] Take the aged larvae of the monochamus alternatus intercepted in the wood imported from Ukraine (see Table 1 for the sample information of the monochamus alternatus larvae), and clean the impurities and feces on the surface of the monochamus alternatus in clean water with a brush , put it on a clean paper to dry, and put it in a clean EP tube for later use;

[0049] Table 1 Sample information of the larvae of Monochamus spruce

[0050]

[0051]

[0052] 3. Freeze drying

[0053] Put the treated old larvae of Alchamona sprucee into EP tubes, and pre-freeze them in a -30°C refrigerator. Then put the spruce Alba bivaria into a freeze dryer for 24 hours of freeze-drying,...

Embodiment 2

[0066] 1. Instruments and reagents

[0067] Instruments: gas chromatography and mass spectrometry (Agilent-7890A); freeze dryer (LABCONCO); nitrogen blowing instrument (HGC-12A)

[0068] Reagent: n-hexane (analytical alcohol) (Sinopharm Chemical Reagent Co., Ltd.)

[0069] 2. Sample preparation

[0070] Take the aged larvae of the monochamus alternatus intercepted from the wood imported from Ukraine (see Table 2 for the sample information of the monochamus alternatus larvae), and clean the impurities and feces on the surface of the monochamus alternatus with a brush in clean water , put it on a clean paper to dry, and put it in a clean EP tube for later use;

[0071] Table 2 Sample information of monochamus spruce larvae

[0072]

[0073]

[0074] 3. Freeze drying

[0075] Put the treated old larvae of Alchamona sprucee into EP tubes, and pre-freeze them in a -30°C refrigerator. Then put the spruce Alba bivaria into a freeze dryer for 24 hours of freeze-drying, the ...

Embodiment 3

[0092] 1. Instruments and reagents

[0093] Instruments: gas chromatography and mass spectrometry (Agilent-7890A); freeze dryer (LABCONCO); nitrogen blowing instrument (HGC-12A)

[0094] Reagent: n-hexane (analytical alcohol) (Sinopharm Chemical Reagent Co., Ltd.)

[0095] 2. Sample preparation

[0096] Take the aged larvae of the long-running monochamus intercepted in the wood imported from Ukraine (see Table 3 for the sample information of the monochamus larvae), and clean the impurities and feces on the surface of the long-tailed monochamus in clean water with a brush , put it on a clean paper to dry, and put it in a clean EP tube for later use;

[0097] Table 3 Sample information of the larvae of Alpinia spruce

[0098] Sample serial number Origin collection date Remark PA-3 Ukraine 2015 Port interception MA-3 Ukraine 2015 Port interception ME-3 Ukraine 2015 Port interception WV-3 Ukraine 2015 Port interception ...

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Abstract

The invention discloses a determination method of longicorn larva epidermis hydrocarbon, the determination method comprises the following steps: step 1, cleaning the surface of longicorn larva clearly; step 2, performing freeze drying; step 3, extracting a sample, wherein the extracting time is 10 to 30min; step 4, concentrating extraction liquid; step 5, performing GC-MS analysis; step 6, performing data analysis on obtained results of the GC-MS analysis, the data analysis comprises qualitative analysis and quantitative analysis. Moreover, the invention also discloses application of the determination method in classification and identification methods and systems of the longicorn larva. The determination method disclosed by the invention has the advantages of easy operation, low cost, short experiment period, thus the human and material resources are greatly saved, the quarantine period is shortened, and the determination method solves the difficult problem that the classification and determination technique is difficult to identify the longicorn larva with hard-to-identified forms.

Description

technical field [0001] The present invention relates to forestry and plant quarantine technical field, be specifically related to the measuring method of the epidermis hydrocarbon of longicorn larvae; Especially relate to the analysis method of the gas-mass spectrometry instrument of the epidermal hydrocarbon component of longicorn larvae; In addition, the present invention It also relates to the application of the method in the classification and identification method and system of longicorn larvae. Background technique [0002] Beetles are the general term for insects of the Coleoptera polyphagous suborder Cyetridae. They have chewing mouthparts and long antennae, which often exceed the length of the body. There are more than 20,000 species in the world. There are some species that are pests, such as the longhorn beetle, long-winged longhorn beetle, etc. The larvae of the longhorn beetle live in the phloem of wood and cause harm to tree growth or buildings. Species of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 王书平苏翠翠朱雅君
Owner PLANTS & ANIMALS & FOOD TESTING QUARANTINE TECH CENT SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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