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Photoelectric detection equipment and detection method thereof

A photoelectric detection and detection method technology, which is applied in the direction of color/spectral characteristic measurement, etc., can solve problems such as inaccurate test results and changes in reaction speed, and achieve the effect of reducing interference and eliminating detection errors

Active Publication Date: 2020-01-17
河北清溪生物科技有限公司
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AI Technical Summary

Problems solved by technology

[0005] According to the nature of the enzymatic reaction, the change of the enzyme activity will lead to the change of the reaction speed, that is, the change of the enzyme activity has a great influence on the determination of the substrate concentration, resulting in inaccurate test results.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Urinary galactose detection equipment and detection method (dual enzyme detection system)

[0023] The urine galactose detection device includes a sampling unit, a detection unit, a calculation unit and a display unit, wherein the detection unit contains two detection heads, the detection target is the reflected light of the reagent card, and the detection reagent card contains two detection holes. The urine galactose detection equipment has a built-in curve of different galactose oxidase concentration-reflection photometry at 1mmol / L galactose concentration, which is used to detect the enzyme activity of the reagent sheet. According to the degree of influence of the attenuation of the enzyme activity of the galactose oxidase reagent tablet during the shelf life on the test results obtained from the test, a different galactose concentration-reflection photometry curve was prepared for every 6% of the attenuation of the enzyme activity, and the attenuation of the enzyme a...

Embodiment 2

[0026] Triglyceride detection equipment and determination method (three-enzyme detection system)

[0027] The triglyceride detection device includes a sampling unit, a detection unit, a computing unit and a display unit, wherein the detection unit contains two detection heads, the detection target is the gray scale of the reagent card, and the detection reagent card contains two detection holes. The triglyceride detection equipment has a built-in curve of different lipase concentration-gray scale under the concentration of 0.9mmol / L triglyceride, which is used to determine the concentration of lipase on the reagent sheet. The triglyceride determination equipment also has a bottle of 0.9mmol / L triglyceride. The linear range of the concentration measured by the triglyceride detection equipment is 0.5-1.4mmol / L. According to the degree of influence of the enzyme activity decay of the lipase reagent tablet during the shelf life on the test results obtained according to the test, ...

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Abstract

The invention relates to the field of biochemical detection, in particular to photoelectric detection equipment and a detection method thereof. The photoelectric detection equipment comprises a sampleinjection unit, a detection unit, an operation unit and a display unit, wherein the detection unit comprises a photoelectric detector with two detection heads and a reagent card with two detection holes; a hole 1 is used for detecting the enzyme activity on a reagent sheet; and the substrate concentration to be measured is detected according to the enzyme activity measured by the hole 1 and the detection result of a hole 2. The enzyme activities of multiple dry chemical reagent sheets decrease along with the increase of storage time (still within the shelf life) or the change of storage conditions, resulting in inaccurate detection results. Through the combined application of the detection equipment and the detection method thereof disclosed by the invention, the influence of the change of the enzyme activity on the reagent sheet on the determination of the substrate concentration can be lowered or even eliminated.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a photoelectric detection device and a detection method thereof. Background technique [0002] The dry chemical enzymatic method is used to determine the substrate concentration, which is easy to operate and takes a short time, and is suitable for rapid inspection projects. [0003] In clinical use, we found that many dry chemical reagent tablets of photoelectric detection equipment will decrease the enzyme activity with the increase of storage time (still within the shelf life) or improper storage conditions (temperature change and humidity change), affecting The test results lead to misdiagnosis. [0004] For example, in dry chemical detection, some tool enzymes with metal ions as active centers, such as polyphenol oxidase and galactose oxidase, have poor stability, and their active centers are easily inactivated by the influence of thiol groups in the molecule. In practi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/27
CPCG01N21/27
Inventor 徐詹程
Owner 河北清溪生物科技有限公司
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