Targeted detection method of silver nanocluster imprinted polymer for tumor marker

A technology of silver nanoclusters and tumor markers, which is applied in the field of silver nanocluster imprinted polymers and its application in the targeted detection of tumor markers, can solve the problems of weak binding ability and achieve good stability and repeatability. Good performance and high sensitivity

Active Publication Date: 2020-01-17
NANTONG UNIVERSITY
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Problems solved by technology

Previous studies have found that i-motif functionalization can regulate the specific binding of aptamers to tumor markers in an acidic environme

Method used

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  • Targeted detection method of silver nanocluster imprinted polymer for tumor marker
  • Targeted detection method of silver nanocluster imprinted polymer for tumor marker
  • Targeted detection method of silver nanocluster imprinted polymer for tumor marker

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Embodiment 1

[0026] 1. Obtaining silver nanoclusters based on smart aptamers

[0027] (1) Optimization of PSA smart aptamer

[0028] ① Transformation of intelligent aptamers. Since the PSA smart aptamer (SP-Apt I) has been obtained in the early stage, as shown in Table 1, SP-Apt I consists of four parts: cytosine region (CCCCCCC), i-motif region (CCC CCT TTT CCC, ACC CCC TTTTCC CCC), linker region (CTA ACT, GGT TAG) and aptamer region (AAT TAA AGC TCG CCA TCA AAT AGC).

[0029] Table 1. SP-Apt I sequence

[0030]

[0031] Microthermophoresis technique (MST) results showed that SP-Apt I could specifically bind to PSA in an acidic environment, but the binding ability of the two was relatively weak in an acidic environment. The specific binding ability of SP-Apt I needs to be optimized. According to literature reports, i-motif regulates the specific binding ability of the aptamer region by affecting the state of the junction region, and the base sequence length of the junction region h...

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Abstract

The invention discloses a silver nanocluster imprinted polymer and an application thereof in targeted detection of tumor markers. Based on a designed PSA intelligent aptamer, specific binding abilityof the PSA intelligent aptamer is improved after optimization and modification. The PSA intelligent aptamer is used as a template to synthesize silver nanoclusters. Combined with specific recognitionadvantages of imprinted polymers, the silver nanocluster imprinted polymer is prepared; and the application of the silver nanocluster imprinted polymer to the targeted recognition of PSA in tumor tissues is explored. The present invention is expected to provide new research ideas for improving the targeted recognition of tumor markers and is of great significance for the clinic targeted diagnosisand treatment of cancers.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a silver nano-cluster imprinted polymer and its application in targeted detection of tumor markers. Background technique [0002] At present, the targeted identification of tumor markers is an important link in the targeted diagnosis and treatment of cancer, and is of great significance for reducing cancer mortality. Traditional target recognition technology is greatly limited in application due to the source of biological antibodies. Nucleic acid aptamers have been widely used in the field of specificity analysis because of their advantages such as easy synthesis, non-toxicity, easy modification, and lack of immunogenicity. However, since most tumor markers are also expressed in normal tissues, aptamers will still target normal tissues, so it is imperative to improve the targeting of aptamers to biomarkers in tumor tissues. The weak acidity of tumor ...

Claims

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Application Information

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IPC IPC(8): G01N33/574C12N15/115
CPCG01N33/57484C12N15/115C12N2310/16
Inventor 丁伟华孙斐顾亚云李文清
Owner NANTONG UNIVERSITY
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