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Tumor cell content assessment method, device, system and computer readable medium

A technology of tumor cells and evaluation devices, which is applied in proteomics, genomics, instruments, etc., can solve problems such as difficulties, lack of unity, and large human errors in pathological staining, and achieve the effect of high proximity

Active Publication Date: 2022-04-22
SHANGHAI ORIGIMED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Depend on figure 1 It can be seen that the results of various methods are very different, and in this case, it is impossible to determine which result is accurate
[0009] It can be seen that in the current analysis methods for the content of tumor cells in these samples, the human error of pathological staining is large, and the results obtained by different methods are quite different, and there is no uniform standard, which brings many difficulties in practical application.

Method used

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  • Tumor cell content assessment method, device, system and computer readable medium
  • Tumor cell content assessment method, device, system and computer readable medium
  • Tumor cell content assessment method, device, system and computer readable medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] This example is to illustrate the tumor cell content evaluation system and the corresponding processing process.

[0057] figure 2 A structural block diagram of the tumor cell content evaluation system involved in Example 1 of the present invention.

[0058] Such as figure 2 As shown, the tumor cell content evaluation system 100 includes: a mutation frequency detection device 10 , and a tumor cell content evaluation device 20 communicatively connected to the mutation frequency detection device 10 through a communication network 30 .

[0059] The mutation frequency detection device 10 is based on the results of sequencing the detection sample and the control sample, and analyzes it to obtain a sequencing sequence comprising each average sample mutation frequency corresponding to each mutation site and a control mutation frequency corresponding to each mutation site. Analyze the data, that is, obtain the mutation frequency of each mutation site in the detection sample...

Embodiment 2

[0096] The following is a description of Embodiment 2.

[0097] In Example 2, the same symbols are assigned to the same structures as in Example 1, and the same descriptions are omitted.

[0098] Figure 5 It is a structural block diagram of the tumor cell content assessment device involved in Example 2 of the present invention.

[0099] Such as Figure 5 As shown, the tumor cell content evaluation device 220 has a single tumor cell content calculation unit 21, a tumor content calculation unit 22, a content evaluation unit 223, an information storage unit 24, a tumor cell content evaluation side communication unit 25, and a tumor cell content evaluation side temporary storage unit. Part 26 and tumor cell content evaluation side control part 27.

[0100] In this embodiment, the difference between the tumor cell content evaluation device 220 and the embodiment is the predetermined evaluation rule adopted by the content evaluation unit 223. The predetermined evaluation rule in...

Embodiment 3

[0106] This example is to verify the reliability of the tumor cell content assessment device in the tumor cell content assessment system in Example 1 and the corresponding processing process for evaluating the tumor cell content in samples. details as follows.

[0107] Sample selection: Select paired tumor cell line samples and normal cell line samples. The purchased commercial cell lines are shown in Table 2. The tumor cell lines are all pure tumor cells, that is, 100% tumor cells, and the normal cell lines are all pure tumor cells. normal cells, that is, 100% normal cells.

[0108]

[0109] Mixing is done in two ways:

[0110] Method 1: Before sequencing (experimental stage), DNA extracted from tumor cell lines and DNA extracted from normal cell lines were mixed according to different ratios. Targeted sequencing.

[0111]

[0112] Method 2: In the data analysis stage, perform data extraction and mixed samples, specifically: first sequence the 5 pure cell lines in Ta...

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Abstract

The present invention provides a tumor cell content assessment method, device, system and computer-readable medium capable of improving the accuracy of tumor cell content assessment, wherein the tumor cell content assessment method includes the following steps: obtaining one by one the tumor cells that meet the predetermined mutation condition The average sample mutation frequency and control mutation frequency of the mutation site, and calculate the content of individual tumor cells corresponding to the mutation site; once the content of all individual tumor cells corresponding to all mutation sites that meet the predetermined mutation conditions are obtained, the calculating the content of all individual tumor cells to obtain the content of tumor cells in the test sample; evaluating the calculated content of tumor cells in the test sample according to a predetermined evaluation rule to obtain the content of tumor cells in the test sample after evaluation.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a method, device, system and computer-readable medium for assessing tumor cell content. Background technique [0002] 1 When detecting and analyzing gene variation in tumor tissue, there are also normal cells in tumor cells. Due to the existence of normal cells, when analyzing gene copy number variation, gene heterozygosity loss, and tumor cell subclones, etc., it is necessary to To get more precise results, tumor cells need to be taken into account. [0003] At present, the analysis of tumor cell content is mainly through pathology, and there are some algorithms such as FACETS and PureCN. Among them, pathology staining is manually performed, and the error is large, and the difference between the other two algorithms is also great. [0004] The following is the use of these methods to analyze the tumor cell content of 38 tumor samples (test samples, Sample). The results of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B20/20
CPCG16B20/20
Inventor 张水荣施巍炜王凯柳文进黄璐嘉
Owner SHANGHAI ORIGIMED CO LTD
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