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Culture cylinder, culture system and culture method for microbial culture

A technology of microbial culture and culture cylinder, which is applied in the direction of biochemical equipment and methods, enzymology/microbiology devices, specific-purpose bioreactors/fermenters, etc., which can solve the problems of strain death, high cost, strain death and pollution and other issues

Pending Publication Date: 2020-03-13
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a method that can avoid the cost caused by the preservation and recovery process of bacteria in view of the high cost caused by the preservation and recovery process of the bacteria in the current microbial culture, and the risk of death and contamination of the bacteria. Microbial culture systems with high risk of strain death and contamination

Method used

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  • Culture cylinder, culture system and culture method for microbial culture
  • Culture cylinder, culture system and culture method for microbial culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1, such as figure 1 and 2 Shown:

[0080] A cylinder for cultivating microorganisms, comprising a cylinder 1 with a closed lower end and an open upper end and a cover 2 matched with the open end of the upper end of the cylinder 1, and the inner space of the cylinder 1 is divided from bottom to top into Culture substrate chamber 3 and gas chamber 4, described culture substrate chamber 3 is used for containing the culture medium suitable for microbial growth, and described gas chamber 4 is used for containing the suitable gas suitable for microbial growth, also is provided with in described barrel 1 A gas replacement device, the gas replacement device is used to replace the gas in the gas chamber 4 with a new suitable gas.

[0081] In the scheme of this application, the culture medium suitable for microorganism cultivation is the current conventional culture medium, which can be a liquid medium, or a solid medium or a colloidal medium. For different types of mic...

Embodiment 2

[0083] Example 2, such as figure 1 and 2 Shown by:

[0084] A gas replacement device for the culture cylinder of the above embodiment, the gas replacement device includes a replacement component and a gas discharge component, the gas discharge component is used to communicate the gas chamber 4 with the external space of the cylinder body 1, the The replacement component is used to push the gas in the gas cavity 4, so that the gas in the gas cavity 4 is discharged out of the barrel 1 by the gas discharge component.

[0085] As a preferred embodiment, the gas exhaust assembly includes a communication pipe 5, one end of which communicates with the gas chamber 4, and the other end communicates with the external space of the cylinder 1 through the cylinder 1 .

[0086] As a preferred embodiment, a cut-off valve is also provided on the communicating pipe 5 . The communication and blocking states of the communication pipe 5 are controlled by the opening and closing of the cut-off...

Embodiment 3

[0104] Example 3, such as figure 1 with 2 Shown by:

[0105] A microbial culture system, including the above-mentioned microbial culture tube, also includes a temperature control cabin 15, the temperature control cabin 15 has a temperature control chamber 16 capable of maintaining a stable temperature in the cavity, all or the lower part of the culture tube As for the inside of the temperature control chamber 16 . In microbial culture, the temperature of the medium and the surrounding environment of the microorganism is also one of the important factors. Whether the temperature is suitable determines the quality of the growth and development of the microorganism. In actual microbial cultivation, some microorganisms will absorb heat, resulting in the temperature of the culture substrate Some microorganisms will release heat, leading to an increase in the temperature of the culture substrate. These changes in temperature often inhibit the further growth and development of micr...

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Abstract

The invention relates to the technical field of microbial culture, specifically to a culture cylinder, a culture system and a culture method for microbial culture. The culture cylinder includes a cylinder body with a closed lower end and an open upper end and a cover body matched with the open upper end of the cylinder body. The inner space of the cylinder body is divided into a culture matrix cavity and a gas cavity from bottom to top. The culture matrix cavity is used for accommodating a culture medium suitable for microbial growth, and the gas cavity is used for accommodating a suitable gassuitable for microbial growth. A gas replacement device is also arranged in the cylinder body, and the gas replacement device is used for replacing a gas in the gas cavity with a new suitable gas. The culture cylinder of the invention reduces the risk of death, variation or contamination during the growth, development and reproduction of microbes, and also ensures a good development speed of microbes and saves culture time.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a cultivation cylinder, a cultivation system and a cultivation method for microorganism cultivation. Background technique [0002] For microorganisms, it is a large group of organisms including bacteria, viruses, fungi, and some small protozoa, which widely exist in the biological world. Although the microorganisms are small, they are closely related to human life. It is widely involved in many fields such as medicine and health, industry and agriculture, and environmental protection. [0003] Over the years, microbiologists have carried out scientific experiments in various aspects such as classification, physiology, and genetics, studied the physiological and biochemical characteristics of microorganisms, and summed up a set of microbial detection, screening and cultivation methods, so that microorganisms can be cultivated according to people's needs. People ...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/04C12M1/00
CPCC12M23/06C12M23/38C12M29/06C12M29/20C12M41/12
Inventor 肖英平吕文涛杨华徐娥王珍刘秀婷
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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