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Culture medium for culturing high-activity plant growth-promoting bacteria and preparation method thereof

A plant growth-promoting bacteria and culture medium technology, applied in the preparation of organic compounds, cyanide reaction preparation, chemical instruments and methods, etc., can solve the problems of low number of viable bacteria and poor pertinence of growth-promoting bacteria, and achieve good pertinence , promote root elongation, high activity effect

Inactive Publication Date: 2020-03-31
沈兰兰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing medium for cultivating plant growth-promoting bacteria (PGPR) has the defects of poor pertinence and low number of viable growth-promoting bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A culture medium for cultivating highly active plant growth-promoting bacteria, wherein the medium for cultivating highly active plant growth-promoting bacteria per 1000mL contains beef extract 10g, peptone 15g, sodium chloride 4.5g, uranyl acetate dihydrate Compound 5g, glycerin 10g and 1-aminocyclopropane-1-carboxylic acid 80mg.

[0021] Wherein, taking the weight of the beef extract as 100wt%, the solid content of the beef extract is 80wt%.

[0022] Among them, the preparation method of 1-aminocyclopropane-1-carboxylic acid is: using nitroacetate and 1,2-dihalogenated ethane as raw materials, through alkylation cyclization, nitro reduction and carboxyl hydrolysis reaction to obtain Crude 1-aminocyclopropane-1-carboxylic acid, and then the crude 1-aminocyclopropane-1-carboxylic acid is separated, purified, and crystallized to obtain high-content and high-purity 1-aminocyclopropane-1-carboxylic acid acid.

[0023] Wherein, every 100 g of the peptone contains 20 g of ...

Embodiment 2

[0031] A culture medium for cultivating highly active plant growth-promoting bacteria, wherein the medium for cultivating highly active plant growth-promoting bacteria per 1000mL contains 8g of beef extract, 20g of peptone, 4g of sodium chloride, and uranyl acetate dihydrate 4g, glycerin 8g and 1-aminocyclopropane-1-carboxylic acid 70mg.

[0032] Wherein, taking the weight of the beef extract as 100wt%, the solid content of the beef extract is 80wt%.

[0033] Among them, the preparation method of 1-aminocyclopropane-1-carboxylic acid is: using nitroacetate and 1,2-dihalogenated ethane as raw materials, through alkylation cyclization, nitro reduction and carboxyl hydrolysis reaction to obtain Crude 1-aminocyclopropane-1-carboxylic acid, and then the crude 1-aminocyclopropane-1-carboxylic acid is separated, purified, and crystallized to obtain high-content and high-purity 1-aminocyclopropane-1-carboxylic acid acid.

[0034] Wherein, every 100 g of the peptone contains 20 g of ...

Embodiment 3

[0042] A culture medium for cultivating highly active plant growth-promoting bacteria, wherein the medium for cultivating highly active plant growth-promoting bacteria per 1000mL contains beef extract 12g, peptone 10g, sodium chloride 5g, uranyl acetate dihydrate 6g, 12g of glycerin and 90mg of 1-aminocyclopropane-1-carboxylic acid.

[0043] Wherein, taking the weight of the beef extract as 100wt%, the solid content of the beef extract is 80wt%.

[0044] Among them, the preparation method of 1-aminocyclopropane-1-carboxylic acid is: using nitroacetate and 1,2-dihalogenated ethane as raw materials, through alkylation cyclization, nitro reduction and carboxyl hydrolysis reaction to obtain Crude 1-aminocyclopropane-1-carboxylic acid, and then the crude 1-aminocyclopropane-1-carboxylic acid is separated, purified, and crystallized to obtain high-content and high-purity 1-aminocyclopropane-1-carboxylic acid acid.

[0045] Wherein, every 100 g of the peptone contains 20 g of trypton...

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PUM

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Abstract

The invention provides a culture medium for culturing high-activity plant growth-promoting bacteria. The formula system contains a beef extract, peptone, sodium chloride, uranyl acetate dihydrate, glycerol and 1-aminocyclopropane-1-carboxylic acid; wherein the addition of the uranyl acetate dihydrate can culture plant growth-promoting bacteria with higher saline-alkaline resistance; the plant growth-promoting bacteria cultured by adding the 1-aminocyclopropane-1-carboxylic acid have higher activity; the PGPB capable of promoting the plant to produce more ACC deaminase (ACCD) secreting more ACCdeaminase can reduce the ethylene level by metabolizing the precursor ACC of ethylene produced by the plant; ethylene endogenous steady state of the plant can be influenced by changing genes for encoding ethylene synthase, ACC synthase and ACC oxidase, and root elongation is promoted.

Description

technical field [0001] The invention belongs to the field of medium preparation, and in particular relates to a medium for cultivating highly active plant growth-promoting bacteria and a preparation method thereof. Background technique [0002] Plant growth promoting rhizobacteria (PGPR) is a class of microorganisms that can promote plant growth, prevent diseases, and increase crop yield. PGPR has a certain biocontrol effect on harmful pathogenic microorganisms in the soil and non-parasitic rhizosphere microorganisms (DRMO for short), and can also promote the absorption and utilization of mineral nutrients by plants, and can produce metabolites that are beneficial to plant growth. Thereby promoting the growth and development of plants. In recent years, many scholars have shown that plant growth-promoting bacteria can indirectly inhibit or alleviate the adverse effects of plant diseases on plants by producing antibiotics and secreting siderophores, and can also produce plant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C07C227/02C07C229/48
CPCC07C201/12C07C227/00C07C227/02C12N1/38C07C205/55C07C229/48
Inventor 沈兰兰
Owner 沈兰兰
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