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Peach fruit acid invertase inhibitor gene ppinh1, encoded protein and its cloning method and application

A technology of acid invertase and gene inhibition, applied in chemical instruments and methods, biochemical equipment and methods, applications, etc., can solve problems that have not been reported, and achieve the effects of reducing chilling damage, reducing decomposition, and inhibiting activity

Active Publication Date: 2022-02-22
NINGBO UNIV
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Problems solved by technology

However, it has not been reported in peach fruit or even other Rosaceae fruit.

Method used

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  • Peach fruit acid invertase inhibitor gene ppinh1, encoded protein and its cloning method and application
  • Peach fruit acid invertase inhibitor gene ppinh1, encoded protein and its cloning method and application
  • Peach fruit acid invertase inhibitor gene ppinh1, encoded protein and its cloning method and application

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Embodiment Construction

[0023] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0024] 1. Gene Cloning of Peach Fruit Acid Invertase Inhibitor Protein

[0025] Five peach fruit suppressor genes were screened from the NCBI database (GenBank accession numbers: XM_007208838, XM_007223513, XM_007209598, XM_007223389, XM_007217302). Primers were designed according to Table 1, and gene cloning and sequence analysis were performed. The sequence results were consistent with those on NCBI.

[0026] Table 1 Primer sequences for PCR amplification of peach fruit suppressor genes

[0027]

[0028] The five suppressor genes were named PpINH1-5. PpINH1 (XM_007208838), PpINH2 (XM_007217302), PpINH3 (XM_007223513), PpINH4 (XM_007223389), PpINH5 (XM_007209598). Bioinformatics analysis found that they all had four α-helices and four conserved cysteine ​​Cys residues, showing the characteristics of typical PMEI-RP protein fa...

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Abstract

The invention discloses peach fruit acid invertase inhibitory gene PpINH1, encoded protein and its cloning method and application. The protein encoded by the suppressor gene PpINH1 has an amino acid sequence as shown in SEQ ID NO: 2. The cloning method includes the following steps: (1) extracting peach fruit total RNA and reverse transcribing it into cDNA as a template; (2) designing according to the gene sequence of PpINH1 Primers; (3) PCR amplification: the PpINH1 gene amplification product is obtained by PCR amplification, which has the advantage of effectively inhibiting the activity of acid invertase and reducing sucrose.

Description

technical field [0001] The invention relates to an acid invertase inhibitory gene, in particular to a peach fruit acid invertase inhibitory gene PpINH1, its encoded protein, its cloning method and application. Background technique [0002] Peach fruit is popular because of its juicy flesh and delicious flavor. However, peach is a respiratory cleave fruit, and the rapid increase of postharvest respiratory intensity will accelerate its ripening and aging, resulting in a short shelf life and rapid decline in flavor. Low temperature can prolong the storage and preservation period of fruits. However, peach is a cold-sensitive fruit. When the storage temperature is lower than 8°C, it is easy to show symptoms of chilling damage. If the meat browns, rots, and flavor changes, the storage period will be shortened, and even the edible value will be lost. . [0003] As the main sugar in the peach ripening process, sucrose is not only closely related to the taste and flavor of the fruit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/10C07K14/415A01H5/00A01H6/74
CPCC07K14/415C12N15/8273
Inventor 邵兴锋韦莹莹王星星王鸿飞许凤姜舒
Owner NINGBO UNIV
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