Peach fruit polygalacturonase inhibitory protein PpPGIP1 gene as well as cloning method and application thereof

A polygalactose and inhibitory protein technology is applied in the field of peach fruit polygalacturonase inhibitory protein PpPGIP1 gene and its cloning to achieve the effects of inhibiting VIN activity, reducing decomposition and reducing chilling damage
CN112662679AActive Publication Date: 2021-04-16NINGBO UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NINGBO UNIV
Publication Date
2021-04-16

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Abstract

The invention discloses a peach fruit polygalacturonase inhibitory protein PpPGIP1 gene as well as a cloning method and an application thereof, and is characterized in that the peach fruit polygalacturonase inhibitory protein PpPGIP1 has a nucleotide sequence as shown in SEQIDNO: 1; the amino acid sequence of the protein encoded by the peach fruit polygalacturonase inhibitory protein PpPGIP1 is shown as SEQIDNO: 2, and the cloning method comprises the following steps: (1) extracting peach fruit total RNA, and carrying out reverse transcription to obtain cDNA as a template; (2) designing primers according to the gene sequence of the PpPGIP1; and (3) PCR amplification: obtaining a PpPGIP1 gene amplification product through PCR amplification. The method has the advantages that PpPGIP1 and PpVIN2 have a protein interaction relationship, the PpPGIP1 expression quantity is effectively inhibited in peach fruits, the activity of acid invertase PpVIN2 can be remarkably reduced, and sucrose decomposition is reduced.
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Description

technical field

[0001] The invention belongs to the field of plant molecular biology, and in particular relates to a peach fruit polygalacturonase inhibitory protein PpPGIP1 gene regulating peach fruit acid invertase PpVIN2, a cloning method and application thereof. Background technique

[0002] Peach( Prunus persica ) belongs to the family Rosaceae and the genus Peach, and is prone to chilling damage at 2-8 °C. The main symptoms include fruit browning, failure to ripen normally, cells under the epidermis shrinking to a spongy shape, and loss of inherent flavor. Low temperature stress is often accompanied by changes in soluble sugar content and metabolism-related enzyme activities. In plants, sucrose is the strongest cell cryoprotectant, which protects plants by regulating osmotic pressure, maintaining the liquid crystal state of cell membrane bilayers and stabilizing proteins. Our previous study showed that under low temperature stress, the degradation of sucrose in peach...

Claims

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