Peach fruit acid invertase inhibitor gene PpINH1, and encoded protein, cloning method and application thereof

An acid invertase and gene inhibition technology, applied in chemical instruments and methods, biochemical equipment and methods, applications, etc., can solve problems such as unreported, and achieve the effects of reducing chilling damage, inhibiting activity, and reducing decomposition

Active Publication Date: 2020-04-14
NINGBO UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

However, it has not been reported in peach fruit or even other Rosaceae fruit.

Method used

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  • Peach fruit acid invertase inhibitor gene PpINH1, and encoded protein, cloning method and application thereof
  • Peach fruit acid invertase inhibitor gene PpINH1, and encoded protein, cloning method and application thereof
  • Peach fruit acid invertase inhibitor gene PpINH1, and encoded protein, cloning method and application thereof

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Embodiment Construction

[0023] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0024] 1. Gene Cloning of Peach Fruit Acid Invertase Inhibitor Protein

[0025] Five peach fruit suppressor genes were screened from the NCBI database (GenBank accession numbers: XM_007208838, XM_007223513, XM_007209598, XM_007223389, XM_007217302). Primers were designed according to Table 1, and gene cloning and sequence analysis were performed. The sequence results were consistent with those on NCBI.

[0026] Table 1 Primer sequences for PCR amplification of peach fruit suppressor genes

[0027]

[0028] The five suppressor genes were named PpINH1-5. PpINH1 (XM_007208838), PpINH2 (XM_007217302), PpINH3 (XM_007223513), PpINH4 (XM_007223389), PpINH5 (XM_007209598). Bioinformatics analysis found that they all had four α-helices and four conserved cysteine ​​Cys residues, showing the characteristics of typical PMEI-RP protein fa...

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Abstract

The invention discloses a peach fruit acid invertase inhibitor gene PpINH1, and an encoded protein, a cloning method and an application thereof. The nucleotide sequence of the peach fruit acid invertase inhibitor gene PpINH1 is represented by SEQ ID NO:1; and the amino acid sequence of the protein encoded by the peach fruit acid invertase inhibitor gene PpINH1 is represented by SEQIDNO:2. The cloning method comprises the following steps: (1) extracting peach fruit total RNA, and carrying out reverse transcription to obtain cDNA used as a template; (2) designing a primer according to the gene sequence of the PpINH1; and (3) carrying out PCR amplification: carrying out PCR amplification to obtain a PpINH1 gene amplification product. The activity of acid invertase can be effectively inhibited, and sucrose is reduced.

Description

technical field [0001] The invention relates to an acid invertase inhibitory gene, in particular to a peach fruit acid invertase inhibitory gene PpINH1, its encoded protein, its cloning method and application. Background technique [0002] Peach fruit is popular because of its juicy flesh and delicious flavor. However, peach is a respiratory cleave fruit, and the rapid increase of postharvest respiratory intensity will accelerate its ripening and aging, resulting in a short shelf life and rapid decline in flavor. Low temperature can prolong the storage and preservation period of fruits. However, peach is a cold-sensitive fruit. When the storage temperature is lower than 8°C, it is easy to show symptoms of chilling damage. If the meat browns, rots, and flavor changes, the storage period will be shortened, and even the edible value will be lost. . [0003] As the main sugar in the peach ripening process, sucrose is not only closely related to the taste and flavor of the fruit...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/10C07K14/415A01H5/00A01H6/74
CPCC07K14/415C12N15/8273
Inventor 邵兴锋韦莹莹王星星王鸿飞许凤姜舒
Owner NINGBO UNIV
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