Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Peach fruit acid invertase inhibitor gene PpINH1, and encoded protein, cloning method and application thereof

An acid invertase and gene inhibition technology, applied in chemical instruments and methods, biochemical equipment and methods, applications, etc., can solve problems such as unreported, and achieve the effects of reducing chilling damage, inhibiting activity, and reducing decomposition

Active Publication Date: 2020-04-14
NINGBO UNIV
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has not been reported in peach fruit or even other Rosaceae fruit.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Peach fruit acid invertase inhibitor gene PpINH1, and encoded protein, cloning method and application thereof
  • Peach fruit acid invertase inhibitor gene PpINH1, and encoded protein, cloning method and application thereof
  • Peach fruit acid invertase inhibitor gene PpINH1, and encoded protein, cloning method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0024] 1. Gene Cloning of Peach Fruit Acid Invertase Inhibitor Protein

[0025] Five peach fruit suppressor genes were screened from the NCBI database (GenBank accession numbers: XM_007208838, XM_007223513, XM_007209598, XM_007223389, XM_007217302). Primers were designed according to Table 1, and gene cloning and sequence analysis were performed. The sequence results were consistent with those on NCBI.

[0026] Table 1 Primer sequences for PCR amplification of peach fruit suppressor genes

[0027]

[0028] The five suppressor genes were named PpINH1-5. PpINH1 (XM_007208838), PpINH2 (XM_007217302), PpINH3 (XM_007223513), PpINH4 (XM_007223389), PpINH5 (XM_007209598). Bioinformatics analysis found that they all had four α-helices and four conserved cysteine ​​Cys residues, showing the characteristics of typical PMEI-RP protein fa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a peach fruit acid invertase inhibitor gene PpINH1, and an encoded protein, a cloning method and an application thereof. The nucleotide sequence of the peach fruit acid invertase inhibitor gene PpINH1 is represented by SEQ ID NO:1; and the amino acid sequence of the protein encoded by the peach fruit acid invertase inhibitor gene PpINH1 is represented by SEQIDNO:2. The cloning method comprises the following steps: (1) extracting peach fruit total RNA, and carrying out reverse transcription to obtain cDNA used as a template; (2) designing a primer according to the gene sequence of the PpINH1; and (3) carrying out PCR amplification: carrying out PCR amplification to obtain a PpINH1 gene amplification product. The activity of acid invertase can be effectively inhibited, and sucrose is reduced.

Description

technical field [0001] The invention relates to an acid invertase inhibitory gene, in particular to a peach fruit acid invertase inhibitory gene PpINH1, its encoded protein, its cloning method and application. Background technique [0002] Peach fruit is popular because of its juicy flesh and delicious flavor. However, peach is a respiratory cleave fruit, and the rapid increase of postharvest respiratory intensity will accelerate its ripening and aging, resulting in a short shelf life and rapid decline in flavor. Low temperature can prolong the storage and preservation period of fruits. However, peach is a cold-sensitive fruit. When the storage temperature is lower than 8°C, it is easy to show symptoms of chilling damage. If the meat browns, rots, and flavor changes, the storage period will be shortened, and even the edible value will be lost. . [0003] As the main sugar in the peach ripening process, sucrose is not only closely related to the taste and flavor of the fruit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C12N15/10C07K14/415A01H5/00A01H6/74
CPCC07K14/415C12N15/8273
Inventor 邵兴锋韦莹莹王星星王鸿飞许凤姜舒
Owner NINGBO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com