A rhd blood group gene rhd993c>t allele and its application

An allele and gene technology, applied in the field of molecular biology, can solve problems such as inability to obtain correct results and difficulty in determining results, and achieve the effect of extensive scientific research and application value.

Active Publication Date: 2021-01-12
WUXI NO 5 PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, serological techniques have certain limitations
Due to the influence of disease or other factors, it is difficult to judge the results of red blood cells in some individuals during serological typing; the serological results of patients with chronic long-term blood transfusion sometimes show the phenomenon of "mixed vision"; when red blood cells cannot be obtained or the red blood cell samples are insufficient, Such as fetal blood type identification, forensic identification of remnants, etc., serological testing can not obtain correct results

Method used

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  • A rhd blood group gene rhd993c>t allele and its application
  • A rhd blood group gene rhd993c>t allele and its application
  • A rhd blood group gene rhd993c>t allele and its application

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Embodiment Construction

[0011] Attached below Figures 1 to 2 The present invention is described further.

[0012] A RhD blood group gene RHD993C>T allele site g.25306649C>T mutation of the present invention. The mutation occurred at position 25306649 of chromosome 1, and the number of this gene in the NCBl reference database GRCh38.p13 is NC_000001.11 (25272393-25330445). The partial nucleotide sequence that contains the wild type of this site in the database is listed here for reference, as shown in SEQ ID NO: 1, and its mutation site is mutated by base C at the 281st position of the sequence of SEQ ID NO: 1 for T. The corresponding sequence of the RHD gene mutation is shown in SEQ ID NO:2.

[0013] SEQ ID NO:1

[0014]

[0015] SEQ ID NO:2

[0016]

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Abstract

The invention discloses an RhD blood group gene RHD993C>T allele and application. A wild type RHD genetic sequence is shown as SEQ ID NO:1, and a mutant RHD genetic sequence is shown as SEQ ID NO:2; and an upstream primer sequence of a specific primer for detecting the RHD993C>T allele is SEQ ID NO:3, and a downstream primer sequence of the specific primer is SEQ ID NO:4. According to the RhD blood group gene RHD993C>T allele and application disclosed by the invention, whether a mutant gene doped in a gene pool exists or not can be detected with high sensitivity and high precision.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a RhD blood group gene RHD993C>T allele and its application. Background technique [0002] The Rh blood group is the most complex and polymorphic system in the human erythrocyte blood group system, and it is also the main erythrocyte blood group that causes clinical transfusion reactions and severe hemolytic disease of the newborn. At present, more than 50 kinds of Rh blood group antigens have been found, among which the RhD antigen has strong immunogenicity and is encoded by the RHD gene, which is the focus of blood group research. Clinically, according to whether the D antigen is detected on the surface of the red blood cell membrane, the Rh blood group antigen is divided into two categories: RhD positive and RhD negative. [0003] At present, the routine detection method of Rh blood group D antigen is identified by serological saline method, indirect antiglobulin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 顾娟邵超鹏王玥苹姬艳丽马静李桢
Owner WUXI NO 5 PEOPLES HOSPITAL
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