Rh blood type DEL type RHD1073T>A allele and application thereof

Abstract

The invention discloses an Rh blood type DEL type RHD1073T>A allele and an application thereof. A wild type RHD gene is represented by SEQ ID NO:1, and a mutant type RHD gene is represented by SEQ IDNO:2. The invention also discloses a specific primer for detecting the mutant gene. The upstream primer sequence of the specific primer is represented by SEQ ID NO:3, the downstream primer sequence ofthe specific primer is represented by SEQ ID NO:4, and the specific primer is used for detecting the RHD1073T>A allele. According to the Rh blood type DEL type RHD1073T > A allele and the applicationthereof, whether mutant genes doped in a gene pool exist or not can be detected in a high-sensitivity and high-precision mode.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a Rh blood type DEL type RHD1073T>A allele and application thereof. Background technique [0002] The Rh blood group is the most complex and polymorphic system in the human erythrocyte blood group system, and it is also the main erythrocyte blood group that causes clinical transfusion reactions and severe hemolytic disease of the newborn. At present, more than 50 kinds of Rh blood group antigens have been found, among which the RhD antigen has strong immunogenicity and is encoded by the RHD gene, which is the focus of blood group research. Clinically, according to whether the D antigen is detected on the surface of the red blood cell membrane, the Rh blood group antigen is divided into two categories: RhD positive and RhD negative. [0003] In 1984, Japanese scholar Okubo et al. further tested the RhD negative samples confirmed by direct agglutination test and indir...

AI Technical Summary

Problems solved by technology

This method is not only cumbersome to operate, but also unstable

In addition, due to the influence of disease or other factors, it is difficult to judge the results of red blood cells in some individuals during serological typing; the serological results of patients with chronic long-term blood transfusion sometimes show the phenomenon of "mixed vision"; Sometimes, such as fetal blood group identification, forensic identification of leftovers, etc., serological tests cannot obtain correct results

[0005] The detection of DEL blood type by immunoserological method depends on the specificity and operation method of the anti-D antibody used, the reliability is low, and it is not suitable for large-scale clinical application due to cumbersome steps. The reliable method is to apply gene Detection method to detect DEL blood type

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