Kit for diagnosing gout and application thereof

A kit and gout technology, applied in the field of medical machinery, to achieve the effect of high diagnosis rate and simple operation

Pending Publication Date: 2020-05-19
广州惠善医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ability to analyze large arrays of metabolites extracts biochemical information that reflects the true functional endpoints of apparent biological events, whereas other functional genomics techniques such as transcriptomics and proteomics, while very valuable, only indicate the potential for phenotypic responses. reason

Method used

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  • Kit for diagnosing gout and application thereof
  • Kit for diagnosing gout and application thereof
  • Kit for diagnosing gout and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] The applicant selected 30 gout patients as the experimental group, and 30 gout-free patients were selected as the control group. The basic information is as follows:

[0017]

[0018] The extracted samples are then processed as follows:

[0019] (1) Sample pretreatment

[0020] 1. Thaw the sample at room temperature, and pipette 100 μL of serum sample into a 1.5 mL EP tube.

[0021] 2. Add 300 μL of methanol to precipitate proteins in serum, and add 10 μL of internal standard (2.9 mg / mL, 2-chlorophenylalanine).

[0022] 3. Vortex and mix for 30 seconds, then place in a centrifuge at 4°C and centrifuge at 12,000 rpm for 15 minutes.

[0023] 4. Aspirate 200 μL of the supernatant and transfer it to a sample injection vial for detection.

[0024] (2) UPLC / MS analysis

[0025] 1. Instrument analysis platform: UPLC-MS (Thermo, Ultimate 3000LC, Q Exactive)

[0026] 2. Chromatographic column: C18 chromatographic column Hypersil GOLD (100×2.1mm, 1.9μm)

[0027] 3. The c...

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Abstract

The invention discloses a kit for diagnosing gout and application thereof, and belongs to the field of medical machinery. The technical scheme provided by the invention is as follows: the kit for diagnosing gout contains standard substances for detecting the contents of pyroglutamic acid, 2-methylbutyryl carnitine and Phe-Phe in a sample. According to the invention, the serum and urine metabonomics characteristics of the gout are identified through LC-MS, three metabolites are discovered, namely pyroglutamic acid, 2-methylbutyryl carnitine, and Phe-Phe, the kit can separate gout patients fromhealth control, can be used as an effective means for early diagnosis, and is simple to operate and high in definite diagnosis rate.

Description

technical field [0001] The invention relates to a kit for diagnosing gout and its application, belonging to the field of medical machinery. [0002] technical background [0003] Gout is a common inflammatory disease characterized by deposition of monosodium urate in arthritic synovial fluid and tissues. In the past few decades, the incidence of gout in the world has continued to increase. Currently, the widely used clinical evaluation and diagnostic criteria for gout It is based on peripheral joint performance and blood uric acid concentration. However, for some patients, serum uric acid concentrations can be normal, even during flare-ups of arthritis. In addition, the serum uric acid level is particularly sensitive to the influence of diet, and the onset of arthritis may be temporary, and it often resolves spontaneously without treatment, which is easily ignored by patients. The early symptoms of gout are mild, and the diagnosis is often delayed in patients with normal ur...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72G01N30/86
CPCG01N30/02G01N30/7266G01N30/8631G01N30/8679
Inventor 杜予和
Owner 广州惠善医疗技术有限公司
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