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Application of sophorolipid in preparation of medicine for preventing against propionibacterium acnes and biofilm thereof

The technology of Propionibacterium acnes and microbial fermentation biosurfactant, which is applied in the application field of medicine, can solve the problems of reducing the curative effect of acne antibiotic treatment and prolonging the antibiotic treatment time, and achieves the effects of significant bacteriostatic effect, low cost and simple process method.

Inactive Publication Date: 2020-05-29
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the extensive use of antibiotics, the efficacy of acne antibiotic treatment is reduced, and the duration of antibiotic treatment is also prolonged

Method used

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  • Application of sophorolipid in preparation of medicine for preventing against propionibacterium acnes and biofilm thereof
  • Application of sophorolipid in preparation of medicine for preventing against propionibacterium acnes and biofilm thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the preparation of sophorolipid

[0037] (1) Strain selection: Wickerhamiella domercqiaevar.sophorolipid CGMCC No.1576;

[0038] (2) Slant culture: Inoculate the strains on a basic inorganic salt medium, add agar with a mass volume ratio of 1.6%, and culture statically for 24 hours at 25°C;

[0039] (3) Seed culture: the bacterial strain cultivated in step (2), under aseptic conditions, connect 1 to 2 loops with an inoculation loop in 20 mL of a medium containing 4% glucose and 1% rapeseed oil in a mass volume ratio , under the condition of 25°C, shake culture for 24 hours to obtain seed solution;

[0040] (4) Expanded cultivation: with an inoculum amount of 5% by volume, the seed solution was inoculated in 100 mL of a medium containing rapeseed oil with a mass volume ratio of 1%, and cultured with shaking for 96 hours at 25°C;

[0041] (5) Collect the fermentation product: leave the fermented liquid obtained in step (4) to allow it to settle naturally, ...

Embodiment 2

[0044] Embodiment 2: the preparation of sophorolipid

[0045] (1) Strain selection: Wickerhamiella domercqiaevar.sophorolipid CGMCC No.1576;

[0046] (2) Slant culture: Inoculate the strains on a basic inorganic salt medium, add agar with a mass volume ratio of 1.6%, and culture statically for 30 hours at 30°C;

[0047] (3) Seed culture: the bacterial strain cultivated in step (2) is inoculated with inoculation loops under aseptic conditions to connect 1 to 2 loops in 50 mL of medium containing 8% glucose and 3% rapeseed oil in a mass volume ratio , under the condition of 30°C, shake culture for 30 hours to obtain the seed solution;

[0048] (4) Expanded cultivation: Inoculate the seed liquid in 500 mL medium containing rapeseed oil with a mass volume ratio of 3% with an inoculation amount of 7% by volume, and culture with shaking at 30°C for 120 hours;

[0049] (5) Collect fermentation product: leave the fermented liquid obtained in step (4) to stand, allow it to settle nat...

Embodiment 3

[0052] Embodiment 3: Sophorolipid as the application of anti-Propionibacterium acnes drug

[0053] Cell preparation of Propionibacterium acnes: Take an inoculation loop of Propionibacterium acnes preserved on the slant and transfer it to liquid anaerobic medium, and culture it in an anaerobic culture bag at 37°C for 36 hours to obtain Propionibacterium acnes Bacterial suspension, diluted to make the concentration of the bacterial suspension 1×10 6 -10 7 CFU / mL.

[0054] The sophorolipid prepared in Example 1 was dissolved in dimethyl sulfoxide (DMSO) to prepare a mother liquor for subsequent use.

[0055] The sophorolipid mother liquor was diluted to different concentrations according to the gradient, and then added to the prepared Propionibacterium acnes suspension (1 × 10 6 -10 7 CFU / mL)

[0056] The culture medium without sophorolipid and the plate with only dimethyl sulfoxide were used as controls.

[0057] The formula of above-mentioned liquid anaerobic culture medi...

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Abstract

The present invention discloses an application of sophorolipid in preparation of a medicine for preventing against propionibacterium acnes and a biofilm thereof. The sophorolipid is prepared from Wickerhamiella domercqiae var. sophorollipid CGMCC No.1576 through a fermentation method; a concentration of the sophorolipid in effectively inhibiting propionibacterium acnes is 0.12-1.5 g / L and a concentration of the sophorolipid in effectively inhibiting propionibacterium acnes biofilm is 2.0-2.5 g / L. Experiments confirm that the sophorolipid has a significant bacteriostatic effect, does not have any toxic and side effects on human body, can be used to replace a conventional antibiotic medicine that is prone to enable people to relapse and infect and have drug resistance after therapy in certain aspects, and has great application prospects.

Description

technical field [0001] The invention relates to a medicinal use of a sophorolipid, in particular to an application of a sophorolipid in preparing medicines against Propionibacterium acnes and its biofilm; it belongs to the technical field of biopharmaceuticals. Background technique [0002] Sophorolipid is an important biosurfactant. The research on it began in the 1950s and 1960s, and it was mainly obtained through microbial fermentation. Compared with chemically synthesized surfactants, sophorolipids have many advantages such as excellent surface properties and environmental friendliness. [0003] In recent years, studies on the antibacterial effect of sophorolipids have been reported, and the applicant has also conducted related research on the antibacterial effect of sophorolipids, among which "CN200710114796.3 Sophorolipids in the preparation of anti-dermatophyte drugs application" is authorized. However, after literature and patent searches, no reports have been foun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7024A61P31/04C12P19/44C12R1/645
CPCA61K31/7024A61P31/04C12P19/44
Inventor 宋欣赵国芹
Owner SHANDONG UNIV
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