Method for culturing and obtaining cordyceps militaris sporophore by using yellow mealworm larvae as raw materials

Abstract

The invention relates to a method for culturing and obtaining cordyceps militaris sporophore by using yellow mealworm larvae as raw materials. The method comprises the steps of inoculating a liquid nutrient medium with cordyceps militaris parent strains, and performing culturing with a total air temperature vibrating shaking table to obtain a liquid strain, wherein the formula of the liquid nutrient medium comprises 18%-25% of potatoes, 1%-4% of glucose, 0.05%-0.2% of KH2PO4, 0.05%-0.2% of MgSO4.7H2O, 0.2%-0.5% of peptone and the balance water; adding yellow mealworm larvae to a culture flask,adding a glucose solution of which the pH is 8 and the concentration is 1%, performing sealing, and performing high-temperature high-pressure sterilization to obtain the culture medium for culturingthe yellow mealworms; uniformly spraying the liquid strain to the surface of the culture medium for culturing the yellow mealworms; and placing the culture flask on a culturing chamber frame, performing culturing, performing picking when ascocarp is densely distributed at the middle and upper part of the sporophore of the cordyceps militaris and spores are not dispersed, and separating yellow mealworm cakes from the cordyceps militaris sporophore, so as to obtain the cordyceps militaris sporophore. The technology is simple, industrial operation is easy to realize, the yellow mealworm larvae are used as main materials for culturing, the obtained cordyceps militaris sporophore is rich in ascocarp and ascospore, and the content of nutrient components of cordycepin, cordyceps sinensis polysaccharide, SOD enzymes and the like is high.

Description

technical field [0001] The invention relates to the field of edible fungus cultivation, in particular to a method for cultivating and obtaining fruiting bodies of Cordyceps militaris by using Tenebrio molitor larvae as raw materials. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, belongs to the genus Cordyceps genus Ergotaceae of the Ascomycetes class Hypocreaes. Because the main active ingredient is similar to or higher than that of Cordyceps sinensis, and has relatively mature cultivation conditions, it has become an effective substitute for Cordyceps sinensis. [0003] In the prior art, CN100392063C discloses the Cordyceps militaris sporocarp with Tenebrio molitor pupae as the host and its cultivation method, the Tenebrio molitor pupae is the host, injection inoculation is inconvenient to operate and is not conducive to factory production, and there are few sporocarps; CN103688760A discloses a A method for cultivating artificial Cor...

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Problems solved by technology

[0003] In the prior art, CN100392063C discloses the Cordyceps militaris sporocarp with Tenebrio molitor pupae as the host and its cultivation method, the Tenebrio molitor pupae is the host, injection inoculation is inconvenient to operate and is not conducive to factory production, and there are few sporocarps; CN103688760A discloses a A method for cultivating artificial Cordyceps using Tenebrio molitor as a carrier. It is inconvenient to operate by injection and inoculation, mainly to harvest infected insects, and the small amount of inoculation causes no fruiting body or is extremely short; CN103749149A discloses a method for using Tenebrio molitor pupae as The method for cultivating artificial Cordyceps with a carrier, which uses pupae as the host, and inoculates by injection only after surface disinfection with alcohol, there is a risk of carrying pathogenic bacteria in the pupae, and the fruiting bodies are short; A method for cultivating Cordyceps products with a carrier, which uses Tenebrio molitor pupae as a host, injects and inoculates, and obtains a product that is a Cordyceps worm without fruiting bodies; CN108184540A discloses a method for cultivating Cordyceps militaris with live Tenebrio molitor larvae as a host, and the obtained fruiting bodies 1.8-3cm, and it is inoculated by injection

[0004] In the above-mentioned related prior art, injection infection is often used to simply obtain the mycelia of Cordyceps militaris. On the one hand, there are cumbersome operations, unsatisfactory biological conversion efficiency, and lack or poor nutritional components of the obtained entity, such as no There are very few fruiting bodies or fruiting bodies and the fruiting bodies are only 1.8~3cm. On the other hand, the existing processing methods are not conducive to industrial production

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