A method to guide the structural rearrangement of cross-linked sodium hyaluronate gel
A technology of cross-linking hyaluronic acid and sodium hyaluronate, which is applied in the field of medical biomaterials to achieve the effects of not being easily collapsed, good viscoelasticity and cohesion, and protecting the integrity of the molecular structure
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Embodiment 1
[0024] Weigh 1 g of sodium hyaluronate dry powder with a molecular weight of 1.3 million Da, dissolve it in 2% sodium hydroxide solution, and prepare a sodium hyaluronate solution with a concentration of 2.4% (m / m). Take 150 μl of divinyl sulfone, dissolve it in 2% sodium hydroxide solution, and prepare a cross-linking agent solution with a concentration of 1.8% (v / v). The sodium hyaluronate solution and the cross-linking agent solution prepared above were injected into microchannels A and B respectively, and the flow rates of the two were adjusted to 60 m / min and 10 m / min, respectively. After the solutions of the two channels enter the mixing channel, they continue to flow at a flow rate of 60 m / min for 15 minutes, and the temperature of the channel is 40°C. Then the preliminarily cross-linked sodium hyaluronate was drained into the cross-linking tank, heated in a water bath at 40° C., and mechanically stirred at 100 rpm for 2 hours. After the reaction is finished, slowly ad...
Embodiment 2
[0027] Weigh 1g of sodium hyaluronate dry powder with a molecular weight of 1.3 million Da, dissolve it in 2% sodium hydroxide solution, and prepare a sodium hyaluronate solution with a concentration of 2.0% (m / m), to which divinyl 150 μl of sulfone, heated in a water bath at 40°C, and mechanically stirred at 100 rpm for 2 hours and 15 minutes. After the reaction is finished, slowly add 95% ethanol solution until a white precipitate is precipitated. The white precipitate is washed 5 to 10 times with the same concentration of ethanol solution, and then filtered to obtain a white powder; the white powder is dissolved in 50ml of PBS solution, dialyzed A cross-linked sodium hyaluronate gel was obtained.
[0028] The gel prepared above was freeze-dried, quenched with liquid nitrogen, and the section of the gel was observed by a scanning electron microscope. like figure 2 As shown, the gel presents an isotropic porous structure.
[0029] Compared with Example 1, under the same c...
Embodiment 3
[0031] Weigh 1 g of sodium hyaluronate dry powder with a molecular weight of 1.7 million Da, dissolve it in 2% sodium hydroxide solution, and prepare a sodium hyaluronate solution with a concentration of 2.0% (m / m). Draw 180 μl of divinyl sulfone, dissolve it in 2% sodium hydroxide solution, and prepare a cross-linking agent solution with a concentration of 1.5% (v / v). The sodium hyaluronate solution and cross-linking agent solution prepared above were injected into microchannels A and B respectively, and the flow rates of the two were adjusted to 80 m / min and 15 m / min, respectively. After the solutions of the two channels enter the mixing channel, they continue to flow at a flow rate of 80 m / min for 8 minutes, and the temperature of the channel is 45°C. Then the preliminarily cross-linked sodium hyaluronate was drained into the cross-linking tank, heated in a water bath at 45° C., and mechanically stirred at 100 rpm for 3 hours. After the reaction is finished, slowly add 95%...
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