Method for guiding rearrangement of cross-linked sodium hyaluronate gel structure
A technology for cross-linking hyaluronic acid and sodium hyaluronate, which is applied in the field of medical biomaterials to achieve the effects of protecting the integrity of molecular structure, improving filling effect, good viscoelasticity and cohesion
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Embodiment 1
[0024] Weigh 1g of dry sodium hyaluronate powder with a molecular weight of 1.3 million Da, and dissolve it in a 2% sodium hydroxide solution to prepare a sodium hyaluronate solution with a concentration of 2.4% (m / m). Take 150 μl of divinyl sulfone and dissolve it in 2% sodium hydroxide solution to prepare a crosslinker solution with a concentration of 1.8% (v / v). Inject the prepared sodium hyaluronate solution and the cross-linking agent solution into the microchannels A and B respectively, and adjust the flow rates of the two to 60 m / min and 10 m / min, respectively. After the solutions in the two channels enter the mixing channel, continue to flow at a flow rate of 60 m / min for 15 minutes, and the channel temperature is 40°C. Then, the preliminarily cross-linked sodium hyaluronate was drained into the cross-linking pool, heated in a water bath at 40°C, and mechanically stirred at 100 rpm for 2 hours. After the reaction is over, slowly add 95% ethanol solution until a white p...
Embodiment 2
[0027] Weigh 1g of dry sodium hyaluronate powder with a molecular weight of 1.3 million Da and dissolve it in a 2% sodium hydroxide solution to prepare a sodium hyaluronate solution with a concentration of 2.0% (m / m), and add divinyl group to it 150μl sulfone, heated in a water bath at 40°C, mechanically stirred at 100rpm for 2 hours and 15 minutes. After the reaction is over, slowly add 95% ethanol solution until a white precipitate is precipitated. The white precipitate is washed 5-10 times with the same concentration of ethanol solution and filtered to obtain a white powder; the white powder is dissolved in 50 ml of PBS solution and dialyzed The cross-linked sodium hyaluronate gel was obtained.
[0028] The gel prepared above was freeze-dried, and after quenching with liquid nitrogen, the cross section of the gel was observed through a scanning electron microscope. Such as figure 2 As shown, the gel exhibits an isotropic porous structure.
[0029] Compared with Example 1, in...
Embodiment 3
[0031] Weigh 1g of dry sodium hyaluronate powder with a molecular weight of 1.7 million Da, and dissolve it in a 2% sodium hydroxide solution to prepare a sodium hyaluronate solution with a concentration of 2.0% (m / m). Take 180 μl of divinyl sulfone and dissolve it in 2% sodium hydroxide solution to prepare a crosslinking agent solution with a concentration of 1.5% (v / v). Inject the prepared sodium hyaluronate solution and the crosslinking agent solution into the microchannels A and B respectively, and adjust the flow rates of the two to 80 m / min and 15 m / min, respectively. After the solutions in the two channels enter the mixing channel, continue to flow at a flow rate of 80 m / min for 8 minutes, and the channel temperature is 45°C. Then, the preliminary cross-linked sodium hyaluronate was drained into the cross-linking pool, and the water bath was heated at 45° C., and mechanically stirred at 100 rpm for 3 hours. After the reaction is over, slowly add 95% ethanol solution unt...
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