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Method for creating triploid rice through combination of hybridization and embryo rescue

An embryo rescue and triploid technology, applied in the field of crop genetics and breeding in modern agriculture, can solve the problem of low efficiency of triploid acquisition

Pending Publication Date: 2020-06-12
HUBEI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for creating rice triploids by combining hybridization and embryo rescue, which solves the problem of low efficiency in obtaining rice triploids in the past, creates rice triploids efficiently, and provides a new way for rice genetic research. and breeding to provide material basis

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  • Method for creating triploid rice through combination of hybridization and embryo rescue
  • Method for creating triploid rice through combination of hybridization and embryo rescue
  • Method for creating triploid rice through combination of hybridization and embryo rescue

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Embodiment Construction

[0032] The invention will be further described below with the creation of the triploid rice A3-3x of the embodiment.

[0033] a. Chromosome doubling in diploid rice

[0034] Using the diploid rice variety A3-2x as the basic material, through the combination of tissue culture and colchicine treatment, chromosome doubling was induced in vitro to obtain A3-4x.

[0035] b. Hybridization of diploid rice and tetraploid rice

[0036] A3-2x and A3-4x were planted at the same time. During the flowering period of the two, A3-2x was clipped and emasculated in the afternoon of the day before pollination, and put into a hybrid bag as the female parent; the next day, the pollen of the male parent A3-4x was pollinated to A3 -2x stigmas.

[0037] c. Embryo rescue

[0038] Ten days after pollination, the hybrid rice panicles were cut, and the number of hybrid spikelets was 2106, of which 454 were enlarged ovaries, and the ovary expansion rate was 21.56%. The enlarged ovaries were peeled of...

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Abstract

The invention discloses a method for creating triploid rice through combination of hybridization and embryo rescue. The method comprises the steps: a. doubling chromosome of diploid rice; b. performing crossing on the diploid rice and tetraploid rice; c. performing embryo rescue; d. differentiating callus for seedling emergence; e. performing rooting culture on bud seedlings; f. transplanting test-tube seedlings; and g. identifying triploid plants. The triploid rice is cultivated through crossing of the diploid rice with the tetraploid rice and adoption of the embryo rescue technique, the problem of low cultivation efficiency of triploid rice in the prior art can be solved through establishment of the technical system, and the triploid rice is created efficiently, so that a material basisis provided for genetic research and breeding of rice.

Description

technical field [0001] The present invention relates to a method for breeding plant polyploids, in particular to a method of hybridizing diploid rice as the female parent and tetraploid rice male parent, combined with embryo rescue for immature embryos to create rice triploids The method belongs to the technical field of crop genetic breeding in modern agriculture. Background technique [0002] Triploid (Triploid) refers to organisms containing three chromosome sets in the body cells. Triploid plants play an important role in genetics research and genetic breeding. In breeding practice, the characteristics of triploid highly sterile can be used to breed seedless fruits, such as triploid seedless watermelon, etc.; or use the growth advantages of some triploid plants to breed fast-growing, high-quality, high-quality fruit. Resistant to new varieties of plants, such as triploid sumac and triploid poplar (Zhang Yingtao and You Ruilin, 1999; Kang Xiangyang, 2003). [0003] Ric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/02
CPCA01H1/02A01H4/001A01H4/008
Inventor 宋兆建屈天歌贺文婷王维张献华蔡得田何玉池刘育华
Owner HUBEI UNIV
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