Application of serum metabolic marker in preparation of diabetic kidney lesion early diagnosis reagent and kit

A technology of metabolic markers and diagnostic kits is applied in the application field of serum metabolic markers in the preparation of early diagnostic reagents and kits for diabetic nephropathy, and can solve the problems of irreversible disease course, decline and decline of glomerular function, etc. Achieve high sensitivity and specificity

Inactive Publication Date: 2020-06-16
ZHEJIANG UNIV
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Problems solved by technology

However, once microalbuminuria is detected clinically, substantial kidney damage already exists, and glomerular function will show a progressive and irreversible decline; and clinically, some DM patients with urinary albuminuria within the normal range have actually dev...

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  • Application of serum metabolic marker in preparation of diabetic kidney lesion early diagnosis reagent and kit
  • Application of serum metabolic marker in preparation of diabetic kidney lesion early diagnosis reagent and kit
  • Application of serum metabolic marker in preparation of diabetic kidney lesion early diagnosis reagent and kit

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Embodiment Construction

[0019] The present invention will be further described below in combination with specific embodiments. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the contents of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0020] The case-control study in the early stage of the present invention selects 44 inpatients with type 2 diabetes mellitus, and is divided into simple DM group (n=20, diagnosed T2DM patients, without any diabetic complication), early DKD group (n=24, diagnosed with In the early stage of DKD, the age, sex, and course of disease were completely matched with those of the control group), after the blood sugar and blood pre...

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Abstract

The invention provides a novel diabetic kidney lesion detection kit, which can identify early-stage patients more sensitively, accurately, simply, conveniently and noninvasively by being compared withtraditional indexes, and can be used as one of novel clinical auxiliary diagnosis means. According to the invention, a UPLC-ESI-MS/MS (ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry) method is used; nine novel serum metabolic markers that are related to early diabetic nephropathy and include hexadecanoic acid, delinoleic acid, azoxystrobin acid, lysophosphatidylcholine (20:4), piperidine, 6-methylmercaptopurine, 6-aminocaproic acid, L-hydrogenated orotic acid and cumin are screened out. Preliminary research data show that the group of metabolitescan diagnose patients with or without early diabetic kidney lesion through single or combined detection, a basis is provided for early intervention treatment of clinicians, and the occurrence of nephropathy at the final stage is reduced to the greatest extent.

Description

technical field [0001] The invention relates to the application of serum metabolites as specific biomarkers in the early diagnosis of diabetic nephropathy. [0002] technical background [0003] Diabetic kidney disease (DKD), formerly known as diabetic nephropathy (DN), is a common microvascular complication in patients with diabetes mellitus (DM). The kidney damage caused by it involves not only blood vessels, glomeruli, and renal tubules, but also almost all renal structures such as renal interstitium and podocytes. [0004] Microalbuminuria (microalbuminuria, MA) and estimated glomerular filtration rate (estimated glomerular filtration rate, eGFR) are currently more recognized indicators for clinical diagnosis of early DKD. However, once microalbuminuria is detected clinically, substantial kidney damage already exists, and glomerular function will show a progressive and irreversible decline; and clinically, some DM patients with urinary albuminuria within the normal range...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/36G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/36G01N30/7266G01N30/8675G01N2030/027G01N2030/8813
Inventor 郑超毛广运张杭谷卫
Owner ZHEJIANG UNIV
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