Application of serum metabolic marker in preparation of diabetic kidney lesion early diagnosis reagent and kit

Abstract

The invention provides a novel diabetic kidney lesion detection kit, which can identify early-stage patients more sensitively, accurately, simply, conveniently and noninvasively by being compared withtraditional indexes, and can be used as one of novel clinical auxiliary diagnosis means. According to the invention, a UPLC-ESI-MS / MS (ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry) method is used; nine novel serum metabolic markers that are related to early diabetic nephropathy and include hexadecanoic acid, delinoleic acid, azoxystrobin acid, lysophosphatidylcholine (20:4), piperidine, 6-methylmercaptopurine, 6-aminocaproic acid, L-hydrogenated orotic acid and cumin are screened out. Preliminary research data show that the group of metabolitescan diagnose patients with or without early diabetic kidney lesion through single or combined detection, a basis is provided for early intervention treatment of clinicians, and the occurrence of nephropathy at the final stage is reduced to the greatest extent.

Description

technical field [0001] The invention relates to the application of serum metabolites as specific biomarkers in the early diagnosis of diabetic nephropathy. [0002] technical background [0003] Diabetic kidney disease (DKD), formerly known as diabetic nephropathy (DN), is a common microvascular complication in patients with diabetes mellitus (DM). The kidney damage caused by it involves not only blood vessels, glomeruli, and renal tubules, but also almost all renal structures such as renal interstitium and podocytes. [0004] Microalbuminuria (microalbuminuria, MA) and estimated glomerular filtration rate (estimated glomerular filtration rate, eGFR) are currently more recognized indicators for clinical diagnosis of early DKD. However, once microalbuminuria is detected clinically, substantial kidney damage already exists, and glomerular function will show a progressive and irreversible decline; and clinically, some DM patients with urinary albuminuria within the normal range...

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Problems solved by technology

However, once microalbuminuria is detected clinically, substantial kidney damage already exists, and glomerular function will show a progressive and irreversible decline; and clinically, some DM patients with urinary albuminuria within the normal range have actually developed The decline of glomerular filtration rate and the characteristic changes related to DKD can also be found in renal pathology; the existing clinical treatment can only slow down the process of DKD to renal failure, and it is far from reversing the course of the disease

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