Drug sensitivity kit

A kit and drug susceptibility technology, which can be used in the determination/inspection of microorganisms, resistance to vector-borne diseases, biochemical equipment and methods, etc., can solve the problems of insufficient accuracy and sensitivity of detection methods, and achieve significant clinical application value and short time. , the effect of biosafety

Pending Publication Date: 2020-06-19
HANGZHOU HANJING BIOTECHNOLOGY CO LTD
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AI Technical Summary

Problems solved by technology

The emergence of a large number of drug-resistant bacteria further shows that the existing detection methods are not accurate and sensitive enough. Therefore, new methods are urgently needed to solve this problem

Method used

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Experimental program
Comparison scheme
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Embodiment Construction

[0048] 1. MH broth liquid medium: Weigh 21g of MH broth powder, fully dissolve in 1000mL deionized water, and autoclave at 121°C for 15 minutes. Other broth powders or basal media can also be used for qualitative susceptibility testing.

[0049] 2. MH broth solid medium: Weigh 21g of MH broth powder and 15g of agar powder, fully dissolve with 1000mL of deionized water, and autoclave at 121°C for 15min.

[0050]3. Medicine plate configuration method

[0051] After the drug is dissolved in water or an organic solvent, the drug solution is sterilized through a 0.22 μm membrane, and the drug solution is diluted and added according to the concentration of the drug plate, freeze-dried, and stored at a low temperature of -20°C until use.

[0052] Gram Positive Bacteria Plate 1

[0053]

[0054]

[0055]

[0056] Gram Positive Bacteria Plate 2

[0057]

[0058] 0.1N HCl solution: Measure 0.9mL of hydrochloric acid, add 100mL of deionized water while stirring with a glas...

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Abstract

The invention discloses a drug sensitivity kit. The kit comprises a medicine plate, a detection reagent and a bacteria culture device. The detection reagent takes EZMTT as a drug sensitivity indicatorand is used for quantitatively and qualitatively analyzing the content of dehydrogenase and NAD (P) H in cells to detect the activity and quantity of bacteria. According to the invention, the EZMTT is used as a drug sensitivity indicator for the first time to quantitatively analyze the content of dehydrogenase and NAD (P) H in cells so as to detect the activity and quantity of the bacteria, and amicroplate reader is used for detecting absorbance so as to indirectly reflect the survival condition of the bacteria; the kit can rapidly detect the MIC value and drug resistance of the bacteria, and sensitively detect information of heterogeneity drug resistance of the bacteria, which cannot be detected by a traditional turbidity method.

Description

technical field [0001] The invention relates to a drug sensitivity kit. Background technique [0002] Bacterial infections are common and unfortunately antibiotic resistance in bacteria has become a serious public health problem worldwide. It prolongs patient hospital stays and greatly increases mortality associated with infectious diseases. Mechanisms of resistance include induction of efflux pumps, DNA transfer from resistant organisms, and spontaneous mutation. Even if spontaneous mutations are estimated to be 1 in 106-108 cells, which would lead to heterogeneous drug resistance, long-term drug treatment can promote the growth of this small group of drug-resistant bacteria. Viable bacteria detection methods cannot detect trace heterogeneous drug-resistant bacteria. Therefore, in addition to developing new drugs to treat multidrug resistance, we can also develop a reliable and sensitive method to detect "hidden" drug resistance. This has the potential to limit the futu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04Y02A50/30
Inventor 阮奔放胡庆丰
Owner HANGZHOU HANJING BIOTECHNOLOGY CO LTD
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