Markers for identifying or assisting in identifying vascular cells and/or vascular cell aging degrees and applications of markers

A vascular cell and auxiliary identification technology, applied in the field of biomedicine, can solve problems such as unoptimistic treatment of cardiovascular diseases and side effects

Active Publication Date: 2020-06-19
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current treatment of cardiovascular diseases is not optimistic. Both drug therapy and surgical treatment have certain risks and side effects, and there are few treatments that treat both symptoms and root causes.

Method used

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  • Markers for identifying or assisting in identifying vascular cells and/or vascular cell aging degrees and applications of markers
  • Markers for identifying or assisting in identifying vascular cells and/or vascular cell aging degrees and applications of markers
  • Markers for identifying or assisting in identifying vascular cells and/or vascular cell aging degrees and applications of markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1. Acquisition of cynomolgus monkey vascular single-cell sequencing library

[0090] According to the average life span of cynomolgus monkeys and its corresponding relationship with human age, select 4 female cynomolgus monkeys aged 4-5 and 4 male cynomolgus monkeys aged 4-5 to form a young group, and 4 monkeys are 18-20 years old The female cynomolgus monkeys and 4 male cynomolgus monkeys aged 18-20 formed the old group. All cynomolgus monkeys undergo a physical examination, including the measurement of basic parameters (such as Body Mass Index (BMI)), the detection of biochemical indicators (such as blood routine, urine routine), brain MRI and ultrasound of important internal organs such as the heart Tested to confirm that all cynomolgus monkeys in the young group and the old group were free from major diseases.

[0091] Schematic diagram of the construction and analysis of cynomolgus monkey single-cell sequencing library, see figure 1 , the specific steps a...

Embodiment 2

[0111] Example 2. Acquisition and detection of vascular cell molecular markers

[0112] 1. Bioinformatics analysis to obtain vascular cell molecular markers

[0113] Taking the cynomolgus monkey genome Macaca_fascicularis_5.0 (Ensemble) as a reference, count the unique alignment read length and split it into different cell data, such as figure 2 Cells shown are aortic endothelial cells, coronary endothelial cells, aortic smooth muscle cells, coronary smooth muscle cells, adventitial fibroblasts, immune cells, and epicardial cells. A single molecule is marked with a unique molecular number, the amplification bias is corrected, and the single-cell expression map of cynomolgus monkey blood vessels is obtained. Search for highly variable genes through the Seurat software package, perform nonlinear dimensionality reduction (t-SNE) on these genes in the measured cells based on the gene group, perform clustering through the Find Clusters function of the Seurat software package and ...

Embodiment 3

[0125] Example 3, Acquisition and Detection of Molecular Markers of Senescent Vascular Cells

[0126] 1. Bioinformatics analysis to obtain molecular markers of aging vascular cells

[0127] Based on the cynomolgus monkey vascular cell sequencing library of the young group and the old group in Example 1, the aging vascular cell molecular markers (|log 2 (fold change)|≥0.5, P<0.05, FDR<0.05).

[0128] The expression of FOXO3 in different types of vascular cells in the young group and the old group was as follows: Figure 5 Shown: the expression levels of FOXO3 in the aortic endothelial cells of the young group and the old group were 2.02±1.74 and 1.21±1.44 (mean±standard deviation); The expression levels were 2.09±1.77 and 1.23±1.64 (mean±standard deviation); the expression levels of coronary artery endothelial cells 2 in the young group and the old group were 1.97±1.71 and 1.25±1.53 (mean±standard deviation ); the expression levels of aortic smooth muscle cells in the young ...

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Abstract

The invention discloses markers for identifying or assisting in identifying vascular cells and / or vascular cell aging degrees and applications of the markers. The invention firstly discloses the applications of IL13RA2, WIF1, PTGS1, POSTN, DES, ACTC1, SORBS2 and PEBP4 in preparation of products that identify or assist in identifying the vascular cells. The invention further discloses the application of FOXO3 in preparation of products for identifying or assisting in identifying the aging degrees of the different vascular cells. The markers provided by the invention play an important role in the screening and identification of the vascular cells, can evaluate vascular aging related diseases such as atherosclerosis, and can detect human vascular function and evaluate the degrees of human vascular aging, thereby providing important molecular indications for the research and intervention of the vascular aging related diseases, especially for the evaluation of human cardiovascular functionand disease diagnosis; and the markers have important application value.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to markers for identifying or assisting in identifying vascular cells and / or vascular cell aging degrees and applications thereof. Background technique [0002] The number of people who die from cardiovascular and cerebrovascular diseases is as high as 15 million every year in the world, and its fatality rate ranks first among various diseases. Aging is a major risk factor for cardiovascular disease, and age-related changes in arterial properties, such as endothelial dysfunction and structural changes, are considered to be the main cause of atherosclerosis. Existing studies have shown that age-related vascular aging often occurs before atherosclerosis, and aging vascular endothelium and vascular smooth muscle cells are also isolated from atherosclerotic plaques, suggesting that vascular aging is the cause of The key cause of cardiovascular diseases such as atherosclerosis, amo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6881C12Q1/6883G01N33/68G01N33/577G01N33/569A61K45/00A61K31/7088A61P9/10A61P9/00
CPCC12Q1/6881C12Q1/6883G01N33/6893G01N33/577G01N33/56966A61K45/00A61K31/7088A61P9/10A61P9/00C12Q2600/158G01N2800/323G01N2800/324G01N2800/325
Inventor 曲静刘光慧汤富酬张维绮张书颜鹏泽王思
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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