Method for preparing PD-1/PD-L1 inhibitor from thalictrum elegans

A technology of PD1-PDL1 and leaflets, which can be used in pharmaceutical formulations, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., and can solve problems such as lack of clear research reports

Inactive Publication Date: 2020-07-07
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no clear research reports on the efficacy of Tangsongcao microphylla

Method used

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  • Method for preparing PD-1/PD-L1 inhibitor from thalictrum elegans
  • Method for preparing PD-1/PD-L1 inhibitor from thalictrum elegans
  • Method for preparing PD-1/PD-L1 inhibitor from thalictrum elegans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of the Crude Extract of the Active Components of Pinus microphylla

[0045] The preparation method of the active ingredient that effectively blocks the combination of PD1-PDL1 is as follows:

[0046] (1) Extraction: select high-quality Echinacea lobata, wash and dry with pure water, first soak it with 60% ethanol for 8 hours, extract twice and filter to obtain the filtrate, recover the solvent under reduced pressure, and add 70% ethanol to the filter residue for ultrasonic extraction twice, each time Extract for 3 hours, concentrate under reduced pressure to obtain 70% ethanol extract; use 83% ethanol to extract once in the same way, concentrate under reduced pressure to obtain 83% ethanol extract, and combine to obtain total ethanol extract. The total ethanol extract is completely dissolved in water at 1:3, and then extracted with ethyl acetate, the ratio of water to ethyl acetate is 0.4:1, and the ethyl acetate part and the aqueous phase extract ...

Embodiment 2

[0052] Example 2 Preparation of Crude Extract of Active Components of Pine Grass Microphylla

[0053] The preparation method of the active ingredient that effectively blocks the combination of PD1-PDL1 is as follows:

[0054] (1) Extraction: select high-quality Echinacea lobata, wash and dry with pure water, first soak it with 74% ethanol for 4 hours, extract twice and filter to obtain the filtrate, recover the solvent under reduced pressure, and add 80% ethanol to the filter residue for ultrasonic extraction twice, each time Extract for 4 hours, concentrate under reduced pressure to obtain 80% ethanol extract; use 85% ethanol to extract once in the same way, concentrate under reduced pressure to obtain 85% ethanol extract, and combine to obtain total ethanol extract. The total ethanol extract is completely dissolved in water at a ratio of 1:4, and then extracted with ethyl acetate, the ratio of water to ethyl acetate is 0.5:1, and the ethyl acetate part and the aqueous phase ...

Embodiment 3

[0060] Example 3 Preparation of Crude Extract of Active Components of Pinus microphylla

[0061] The preparation method of the active ingredient that effectively blocks the combination of PD1-PDL1 is as follows:

[0062] (1) Extraction: select high-quality Echinacea lobata, wash and dry with pure water, first soak it with 75% ethanol for 4 hours, extract twice and filter to obtain the filtrate, recover the solvent under reduced pressure, and add 80% ethanol to the filter residue for ultrasonic extraction twice, each time Extract for 2 hours, concentrate under reduced pressure to obtain 80% ethanol extract; use 85% ethanol to extract once in the same way, concentrate under reduced pressure to obtain 85% ethanol extract, and combine to obtain total ethanol extract. The total ethanol extract is completely dissolved in water at a ratio of 1:4, and then extracted with ethyl acetate, the ratio of water to ethyl acetate is 0.8:1, and the ethyl acetate part and the aqueous phase extract...

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Abstract

The invention discloses an active component extracted from a natural plant, namely thalictrum elegans and an extraction method for the active component. More specifically, the active component extracted from the thalictrum elegans can effectively block combination of PD1-PDL1, so the interaction of the PD1-PDL1 and the subsequent action of the PD1-PDL1 are eliminated, inhibited, reduced or blocked. The pharmaceutical composition of the active component and a pharmaceutical carrier can effectively become a novel PD1-PDL1 inhibitor; and the novel PD1-PDL1 inhibitor can be applied to resisting tumor related diseases, and can provide a reference for a clinical treatment scheme.

Description

Technical field: [0001] The present invention relates to the technical field of application of natural products, and extracts active ingredients from the natural plant Pinus microphylla, more specifically relates to extracting active ingredients capable of eliminating, inhibiting, reducing or blocking the combination of PD1-PDL1 and the active ingredient from Pinus microphylla The pharmaceutical composition of the pharmaceutically acceptable carrier, and the application on related diseases. Background technique: [0002] The body's immune system can identify tumor cells, regulate tumor growth, and even eliminate tumors, and T cells play a central role in this process. The activation of T cells first requires the first signal stimulation provided by antigen-presenting cells, and also requires the second signal stimulation provided by co-stimulatory molecules. Costimulatory molecules not only provide co-stimulatory signals to enhance immunity, but also provide co-inhibitory s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61P35/00A61P35/02
CPCA61K36/71A61K2236/333A61K2236/39A61K2236/55A61P35/00A61P35/02
Inventor 何彦峰吴疆张金魁林鹏程王敏姚政裴荣冠
Owner QINGHAI UNIV FOR NATITIES
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