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A test strip for early screening of esophageal squamous cell carcinoma

A technology of esophageal squamous cell carcinoma and test strips, which is applied in the field of medicine and biology, can solve problems such as poor prognosis, structural differences, and low survival rate, and achieve the effects of reducing mortality, high sensitivity and specificity, and high detection accuracy

Active Publication Date: 2020-12-08
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the structures of NME1, NME2, and NME3 are similar, there are certain differences in their structures. It is worth noting that studies have shown that low expression of NME1 is associated with poor prognosis, low survival rate, and lymph node infiltration of patients. Tumor histopathological indicators

Method used

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  • A test strip for early screening of esophageal squamous cell carcinoma
  • A test strip for early screening of esophageal squamous cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: the preparation of test strip

[0049] 1. Experimental materials

[0050] The bioactive raw materials used in the present invention are all commercially available products. Among them, MPST monoclonal antibody, NME1 monoclonal antibody, MPST polyclonal antibody, and NME1 polyclonal antibody were all purchased from Wuhan Aimeijie Biotechnology Company, the article numbers are: H00004357-M01, H00004830-M01, H00004357-B01, H00004830- A01.

[0051] PVC base plate, nitrocellulose membrane, glass fiber membrane, absorbent paper etc. are all existing commercially available products.

[0052] 2. Preparation of binding pads

[0053] (1) Preparation of colloidal gold:

[0054] A colloidal gold solution with a diameter of 25-35nm was prepared by a trisodium citrate reduction method. Add 300mL of 0.01% chloroauric acid solution into the cleaned flask and heat to boiling. Quickly add 4.8mL of 0.1% trisodium citrate aqueous solution under magnetic stirring, contin...

Embodiment 2

[0078] Embodiment 2: the diagnostic value analysis of test strip

[0079] Use the test strip prepared in Example 1 of the present invention to detect the serum samples of patients with pathologically confirmed early esophageal squamous cell carcinoma and normal people, and evaluate and analyze the value of the test strip of the present invention for screening and diagnosis of early esophageal squamous cell carcinoma.

[0080] 1. Sample source

[0081] Serum samples were collected from the Henan Key Open Laboratory of Esophageal Squamous Cell Carcinoma in the First Affiliated Hospital of Zhengzhou University, including 160 samples of normal human serum (control group) and 160 serum samples of patients with early-stage esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group). Among the 160 normal subjects, there were 83 males and 77 females, with an average age of 55.8±9.1 years and a range of 39-80 years. Among the 160 patients with esophageal squamous cel...

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Abstract

The invention belongs to the technical field of medical biology and discloses a test strip for screening the early esophageal squamous cell carcinoma. The test strip for screening the early esophagealsquamous cell carcinoma comprises a combination pad and a chromatography pad, the combination pad is coated with a capture antibody, the capture antibody is a mixture of an MPST antibody A and an NME1 antibody A, and the MPST antibody A and the NME1 antibody A are provided with detectable markers; two detection lines and a quality control line are arranged on the chromatography pad, detection antibodies are fixed on the detection lines, the detection antibodies fixed on the two detection lines are respectively an MPST antibody B and an NME1 antibody B, the antibody A and the antibody B of thesame antigen recognize different epitopes of the antigen; goat anti-mouse IgG is fixed on the quality control line. The test strip provided by the invention can rapidly detect MPST protein and NME1 protein in human serum, realizes combined detection of two tumor markers, can effectively detect esophageal squamous cell carcinoma, especially early esophageal squamous cell carcinoma, and greatly improves the detection rate of early esophageal squamous cell carcinoma.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a test strip for screening early esophageal squamous cell carcinoma. Background technique [0002] Esophageal squamous cell carcinoma (ESCC) is a squamous cell carcinoma that occurs in human esophageal epithelial tissue, a malignant tumor of the digestive tract, and is very common in clinic. Esophageal squamous cell carcinoma will not only reduce people's quality of life, endanger people's life and health, but also bring heavy disasters to patients' families and a heavy burden to the country. Esophageal squamous cell carcinoma is a common malignant tumor of the digestive system worldwide, and there are typical high-incidence areas of esophageal squamous cell carcinoma in all countries in the world. The high-incidence areas of esophageal squamous cell carcinoma in China have a prominent clustering characteristic. Over the years, many typical high-incidenc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/558G01N33/543
CPCG01N33/54313G01N33/558G01N33/57407G01N33/57488
Inventor 王立东吉佳佳范宗民赵学科宋昕雷玲玲孟超龙李贝韩雪娜王苒郑颖娟
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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