A rapid detection kit for simultaneous detection of multiple transgenic rapeseed lines and its application

Abstract

The invention relates to a PCR micro-fluidic chip special for identification of transgenic rapeseed strains. According to the PCR micro-fluidic chip special for identification of the transgenic rapeseed strains, a series of common transgenic rapeseed element PCR amplification primers, with high specificity and high accuracy, suitable for the micro-fluidic chip are obtained through designing and screening, and through freeze-drying, the primers and the PCR micro-fluidic chip are combined, so that high-throughput screening detection of an endogenous gene and multiple exogenous genes is achievedin a primary PCR amplification reaction. The provided PCR micro-fluidic chip with the pre-cryopreserved primers has good stability in both a normal temperature situation and a situation of repeated freezing and thawing, and has good commercial application value.

Description

technical field [0001] The invention relates to the technical field of agricultural transgene detection, in particular to a microfluidic fluorescent PCR chip kit containing a primer set capable of simultaneously screening 10 transgenic rapeseed strains and an application thereof. Background technique [0002] Genetically modified crops began to be commercially planted on a large scale in 1996, and the global planting area reached 189.8 million hectares in 2017. GMO crops have been accompanied by mixed voices since they were first commercialized. On the one hand, genetically modified crops have indeed greatly increased the yield of crops, reduced the use of pesticides, and protected the environment; but on the other hand, the potential uncertainties caused by genetically modified crops to the environment, society and economy have been questioned in many ways. Therefore, it has become an international practice to strengthen the safety supervision of genetically modified produ...

AI Technical Summary

Problems solved by technology

Ordinary PCR method has been gradually replaced by real-time fluorescent PCR detection method due to its shortcomings such as cumbersome operation, polluted environment, and low sensitivity.

However, the detection cost of single-channel TaqMan real-time fluorescent PCR method is high, and the steps are cumbersome, and it is not suitable for multi-line screening detection of genetically modified products.

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