Sea island cotton GbCYP72A2 gene as well as encoding protein and application thereof

Abstract

The invention discloses a sea island cotton GbCYP72A2 gene as well as an encoding protein and application thereof. The first base of the 5' end of the gene is a transcription starting site, 1558-1560th bases are transcription termination codons, a coding frame has 1557 bases, 519 amino acids are coded in total, the gene has 97% similarity with a homologous gene GbCYP72A1, but 3% of sequence difference can endow plants with higher verticillium wilt resistance, disease-resistant gene resources can be enriched, an effective new tool is provided for cotton verticillium wilt resistance breeding, sensitivity of the plants to ABA in the germination period can be reduced through gene overexpression, and growth of the plants can be promoted through down-regulation expression of the gene.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the sea-island cotton GbCYP72A2 gene, its encoded protein and its application in plant resistance to verticillium wilt. Background technique [0002] Cotton is one of the important economic crops, and cotton fiber is the main raw material of textile industry. With the reduction of arable land and the deterioration of the environment, cotton production is affected by a variety of biological and abiotic factors, especially the frequent occurrence of Fusarium wilt and Verticillium wilt has caused great losses to my country's cotton production. It is difficult to meet the needs of modern agriculture through traditional breeding methods, so improving existing varieties through genetic engineering will be the main method of breeding in the future. Previous studies have shown that the resistance mechanism of cotton Verticillium wilt involves a variety of signaling pathways and ...

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However, the molecular function and mechanism o

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