Screening method of polymorphic SNP molecular markers, polymorphic SNP molecular markers and primer pair

Abstract

The invention relates to the technical field of SNP molecular markers, and particularly relates to a screening method of polymorphic SNP molecular markers. The screening method of the polymorphic SNPmolecular markers comprises the following steps: step one, comparing resequencing with reference genome data so as to obtain candidate SNP sites; and step two, carrying out competitive allele specificPCR amplification and fluorescence typing so as to identify the SNP molecular markers with polymorphism and clear types. The polymorphic SNP molecular markers and the primer pair comprise at least one of SNP molecular marker No. 1 to SNP molecular marker No. 19. The invention solves the problem of time-consuming and labor-consuming process of polymorphic SNP molecular marker screening. The commongiant panda polymorphic SNP molecular markers obtained by the screening method provide 19 effective SNP molecular markers, and supplement the library of SNP molecular markers of the giant panda.

Description

technical field [0001] The invention relates to the technical field of SNP molecular markers, in particular to a screening method for polymorphic SNP molecular markers, polymorphic SNP molecular markers and primer pairs. Background technique [0002] Single nucleotide polymorphism (Single Nucleotide Polymorphism, SNP) widely exists in the genomes of animals and plants, and it is a DNA sequence polymorphism caused by a single nucleotide variation, including single base transitions, transversions, insertions and deletions , is a new generation of molecular markers after microsatellite markers. Compared with microsatellite markers, SNP has a large distribution density, a large number, and rich polymorphism; the mutation rate is low, and the stability and accuracy are high; there is no need to detect the length of the fragment, only need to detect "presence or absence" to determine the polymorphism, This makes it easy to automate screening. [0003] SNP markers have the follow...

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Problems solved by technology

[0006] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a high-throughput screening method for polymorphic SNP molecular markers, which is used to solve the time-consuming and laborious problems in the screening process of polymorphic SNP molecular markers in the prior art, Simultaneously, the present invention will also provide a kind of general polymorphic SNP molecular marker of giant panda; In addition, the present invention will also provide a kind of primer pair that is used for the general polymorphic SNP molecular marker of giant panda

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