Hypsizigus marmoreus bacterial strain and production method of hypsizigus marmoreus fruit body

A technology of shimeji mushrooms and strains, applied in fungi, botanical equipment and methods, mushroom cultivation, etc., can solve the problems of lowering the cultivation temperature, lowering the production efficiency of fruiting bodies, unclear causes or mechanisms of inoculum sclerosis, etc. Achieving the effect of a simple screening method

Inactive Publication Date: 2011-10-05
YUKIGUNI MAITAKE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This phenomenon is known as inoculum sclerosis and is an important problem because it reduces the production efficiency of fruiting bodies
However, the cause or mechanism of inoculum sclerosis is not clear, and as countermeasures, strategies such as lowering the culture temperature have only been tried (for example, non-patent documents 1 and 2)

Method used

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  • Hypsizigus marmoreus bacterial strain and production method of hypsizigus marmoreus fruit body
  • Hypsizigus marmoreus bacterial strain and production method of hypsizigus marmoreus fruit body
  • Hypsizigus marmoreus bacterial strain and production method of hypsizigus marmoreus fruit body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0133] 62 g of sawdust (fir wood) in dry weight, 60 g of rice bran (moisture content 10%) in wet weight, 30 g of corn cob powder (10% in water content) in wet weight, and 20 g of soybean hulls in wet weight (moisture content 10%), wheat bran (moisture content 10%) that the wet weight is 20g is fully mixed, with 2.5g / bottle or 15g / bottle (relative to the total dry weight of the culture medium of solid state, with dry weight ratio 1.1% by weight, 6.4% by weight, respectively) were added to the mixture obtained by adding magnesium aluminum silicate (18% dry loss, produced by Fuji Chemical Industry Co., Ltd., trade name: Noysilin), and the moisture content After adjusting to 65%, it was press-packed in a polypropylene wide-mouth culture bottle of 850 mL capacity (ブロビビン S-850, manufactured by Shin-Etsu Agricultural Supplies Co., Ltd.). In the center of the surface of the pressed product, an inoculation hole with a diameter of about 1 cm is opened, and the lid is closed, then autocl...

Embodiment 2

[0149] The following discusses whether the temperature of cultivating mycelium when adding 15g of aluminum magnesium silicate has an effect on the selection of strains that are not prone to or do not occur inoculum sclerosis.

[0150] Using a solid cultivation medium to which 15 g of magnesium aluminum silicate was added, the temperature at the time of cultivating mycelium was set at 25°C and 27°C, and the same method as that described in Example 1 was adopted. , for Hypsizygus marmoreus K-4975(FERM BP-11321), K-4979(FERM BP-11322), K-4980(FERM BP-11323), K-4981(FERM BP-11324), K -4529, a commercially available strain for comparison, and Ulmusia bursa M-8171 (FERM BP-1415) were studied. First, check the state of the fungus bed surface when scratching the fungus. The results are shown in Table 4.

[0151] [Table 4]

[0152]

[0153] As can be seen from Table 4, under the condition of adding 15 g of magnesium aluminum silicate and the culture temperature of the mycelia is ...

Embodiment 3

[0164] Adopt the method identical with embodiment 2, to true Ji mushroom (Hypsizygus marmoreus) K-4975 (FERM BP-11321), K-4979 (FERM BP-11322), K-4980 (FERM BP-11323), K-4981 (FERM BP-11324), K-4529, commercially available bacterial strains used for comparison, and Ulmus unripe mushroom M-8171 (FERM BP-1415) were studied, and the difference was that 5g / bottle (relative to solid state The total dry weight of the culture medium is about 2.5% by weight in terms of dry weight ratio) of aluminum oxide magnesium hydroxide (co-precipitated product of aluminum hydroxide and magnesium hydroxide, drying loss 10%, Kyowa Chemical Industry Co., Ltd. Produced by the company, trade name: キヨ一ワ一ド300) to replace magnesium aluminum silicate. First, check the state of the fungus bed surface when scratching the fungus. The results are shown in Table 7.

[0165] [Table 7]

[0166]

[0167] As can be seen from Table 7, under the conditions of adding 5 g of alumina magnesium hydroxide and the c...

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Abstract

The invention provides a hypsizigus marmoreus bacterial strain not prone to suffering from inoculum sclerosis, a method of producing hypsizigus marmoreus fruit body through the bacterial strain and a method of screening hypsizigus marmoreus bacterial strain not prone to suffering from inoculum sclerosis. The invention provides a hypsizigus marmoreus bacterial strain and a method of producing hypsizigus marmoreus fruit body through the bacterial strain, wherein, the inoculum part of a solid cultivating substrate surface is not subject to browning when the hypsizigus marmoreus bacterial strain is cultivated in the solid cultivating substrate which is mainly made of conifer sawdust and rice bran and comprise, by dry weight, 6.4 percent of magnesium aluminum silicate. The invention further provides a method of screening hypsizigus marmoreus bacterial strain not prone to suffering from inoculum sclerosis, which makes use of the solid cultivating substrate mainly made of conifer sawdust and rice bran and having a large amount of magnesium compounds. By the screening method in the invention, hypsizigus marmoreus bacterial strains not prone to suffering from inoculum sclerosis can be screened in advance. Thus the yield rate of the hypsizigus marmoreus fruit body can be ensured in large-scale commercial production. Qualified hypsizigus marmoreus fruit bodies can be effectively produced.

Description

technical field [0001] The present invention relates to a strain of shimeji mushroom which is less prone to inoculum sclerosis, and a method for producing fruiting bodies of shimeji mushroom using the strain. Furthermore, the present invention relates to a method for screening a strain of shimeji mushroom that is less likely to develop inoculum sclerosis. Background technique [0002] In recent years, artificial cultivation techniques of mushrooms have been developed to provide fruiting bodies of various edible mushrooms (shiitake mushrooms, enoki mushrooms, oyster mushrooms, slide mushrooms, maitake mushrooms, shimeji mushrooms, lotus leaf mushrooms, shiitake mushrooms, etc.). [0003] Generally, the artificial cultivation methods of mushrooms include log cultivation and fungal bed cultivation. In log cultivation, logs such as oak, zelkova, and eucalyptus are used as broken wood (ほだ wood); Cultivation medium, the solid cultivation medium is formed by mixing nutrient source...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/40C12N1/14
Inventor 前田真奈美小岛理惠日下部克彦
Owner YUKIGUNI MAITAKE
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