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application of PIEZO1 protein as esophageal cancer marker

An esophageal cancer and marker technology, which is applied in the application field of PIEZO1 protein as an esophageal cancer marker, can solve the problems of poor prognosis and low overall survival rate of esophageal cancer, and achieve the effects of inhibiting proliferation, down-regulation of volume and weight, and inhibiting growth.

Active Publication Date: 2020-10-09
河南省医药科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Many studies on esophageal cancer have been reported. By activating the EMT pathway, the PI3K pathway and the P53 signaling pathway can affect the proliferation, invasion and migration of esophageal cancer, but the prognosis of esophageal cancer is still poor, and the overall survival rate within 5 years As low as 20%; so it is urgent to seek new targets and strategies for the treatment of esophageal cancer

Method used

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Embodiment 1

[0034] Download the RNA-Sep data of PIEZO1 expression in esophageal cancer from the public database Xena (http: / / xena.ucsc.edu / ,04 / 6 / 2019). The sample data contains 162 cancer samples and 11 normal samples. By normalizing the data and drawing a scatter diagram, the scatter diagram of the expression of PIEZO1 in esophageal cancer in the TCGA database is obtained, as shown in figure 1 As shown, indicating that PIEZO1 is highly expressed in esophageal carcinoma.

[0035] Purchase esophageal cancer tissue chip array, 49 cases of cancer samples and 25 cases of normal samples. Piezo1 protein immunohistochemical analysis was performed on the tissue chip (Piezo1 immunohistochemistry was produced and synthesized by Shanghai Xinchao Biotechnology Co., Ltd.), and the expression levels were divided into 4 levels according to the sample score: negative (-: score is 0); weak (+ : score of 0.5); strong (++: score of 1); positive (+++: score of 2). Using SPSS16.0 software, using statistica...

Embodiment 2

[0039] Preparation of Piezo1 protein interfering RNA and Piezo1 inhibiting lentivirus

[0040] (1) Interference target design and primer synthesis: According to the general principles of shRNA design, an siRNA target was designed.

[0041] The synthetic sequence of Piezo1 lentivirus is GCCTCGTGGTCTACAAGAT (SEQ ID NO.3).

[0042] The synthetic sequence of NC lentivirus is TTCTCCGAACGTGTCACGT (SEQ ID NO.4).

[0043] The packaging of lentivirus was completed by Shanghai Heyuan Biotechnology Co., Ltd.

[0044] Piezo1 gene knockdown cell line construction

[0045] RT-qPCR verified five kinds of esophageal cancer cells (EC109, EC9706, TE-1, KYSE30, KYSE510) and found that the expression of PIEZO1 protein was relatively high in EC109 and EC9706 cells ( image 3 ), inhibited the expression of Piezo1 protein in these two cells. The previously constructed Piezo1 inhibitory lentivirus was used to transfect the two kinds of cells, and a stable protein expression inhibitory cell line w...

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Abstract

The invention provides an application of a PIEZO1 protein as an esophageal cancer marker, which belongs to the technical field of esophageal cancer detection and treatment. The expression quantity ofthe PIEZO1 protein in esophageal cancer tissues is significantly higher than that of the PIEZO1 protein in para-carcinoma tissues; the esophageal cancer can be detected in an assisted manner by detecting the expression quantity of the PIEZO1 protein; the esophageal cancer can be treated by reducing the expression quantity of the PIEZO1 protein; proliferation, migration and invasion of in-vitro esophageal cancer cells can be inhibited by reducing the expression quantity of the PIEZO1 protein, and growth of in-vivo tumors can be inhibited; according to the record of the embodiment, after the expression quantity of the PIEZO1 protein is reduced, the volume and the weight of in-vivo tumors are obviously reduced.

Description

technical field [0001] The invention belongs to the technical field of detection and treatment of esophageal cancer, and in particular relates to the application of PIEZO1 protein as a marker of esophageal cancer. Background technique [0002] Esophageal cancer is the eighth most malignant cancer and the sixth leading cause of cancer death worldwide. Every year, there are more than 480,000 newly diagnosed patients and about 400,000 deaths. Esophageal cancer can be divided into two types according to histology: esophageal squamous cell carcinoma and esophageal adenocarcinoma. 80% of them are esophageal squamous cell carcinoma. The main predisposing factors of esophageal squamous cell carcinoma are tobacco, alcohol, hot drinks and poor diet. So far, the incidence of esophageal cancer is still rising, and the threat to human beings is gradually increasing. Eighty percent of patients are diagnosed at an advanced stage, and most patients are inoperable because a large proport...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574A61K45/00A61P35/00A61P35/04
CPCG01N33/57484G01N33/57407A61K45/00A61P35/00A61P35/04
Inventor 张丽果高璐陈慧平何美霞代丽萍汲振余张建营刘静静张灿吉云完迪迪孙旭杨小静裘一兵刘晓洁
Owner 河南省医药科学研究院
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