Biological aerosol online monitoring system and method based on ATP biochemical luminescence

Abstract

The invention discloses a biological aerosol online monitoring system and method based on ATP biochemical luminescence. Active microorganism aerosol in ambient air is collected by using a large-flow biological aerosol sampling technology, microbial cells contained in the aerosol are cracked to release ATP by combining an ATP biochemical luminescence reaction principle, the ATP chemically reacts with fluorescein under the catalysis of luciferase to emit light, a light emitting signal is converted into an electric signal by a photoelectric sensor, and the electric signal is recorded and output,so that rapid enrichment and real-time detection of low-concentration active microorganisms in the air are realized.

Description

technical field [0001] The invention relates to a bioaerosol real-time on-line monitoring device, in particular to an on-line bioaerosol monitoring research method and system based on the ATP biochemiluminescent reaction principle combined with large-flow bioaerosol sampling technology. Background technique [0002] Ambient air contains bioaerosols and other pollutants composed of a large number of biological particles, including bacteria, fungi, viruses, animal and plant debris, among which bioaerosols are more harmful to human health and public safety than other non-biological pollutants. direct and significant impact. Exposure to bioaerosols may lead to respiratory inflammation, allergies, infections, and toxic reactions, and even cause large-scale epidemics, so the monitoring of bioaerosols in the environment is particularly important. However, the ambient air microbial detection methods commonly used in environmental monitoring, such as microbial cultivation, have larg...

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Problems solved by technology

However, this principle is limited by the interfering substances in the actual air, such as the interference fluorescence of benzene series substances and the complex particle aggregation structure, which may cause difficult to estimate false positives or false negatives, and it is difficult to accurately and reliably reflect the real ambient air microbial concentration.

In addition, the method of laser-induced fluorescence detection is usually only used in low-flow and small-flow air microbial background monitoring situations. Due to the limitation of too small sampling gas flow, it is impossible to make immediate and effective responses to sudden microbial exposure events. Therefore, it is difficult to meet the requirements of microbial aerosol exposure early warning

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