Anti-fatigue health-care product prepared from deer blood polypeptide and Chinese wolfberry polysaccharide

A deer blood polypeptide and anti-fatigue technology, applied in the direction of polysaccharide/gum-containing food ingredients, blood protein components, applications, etc., can solve problems such as poor palatability, difficult absorption and digestion, and getting angry

Pending Publication Date: 2020-10-27
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the deer blood dry powder is directly consumed as an anti-fatigue product, the bloody smell is heavy and the palatability is poor; in addition, it is not easy to be absorbed and digested by the human body, res

Method used

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  • Anti-fatigue health-care product prepared from deer blood polypeptide and Chinese wolfberry polysaccharide
  • Anti-fatigue health-care product prepared from deer blood polypeptide and Chinese wolfberry polysaccharide
  • Anti-fatigue health-care product prepared from deer blood polypeptide and Chinese wolfberry polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Screening of Deer Blood Polypeptide Preparation Method

[0024] Take 10kg of deer blood, after high-speed shearing and homogenization, add different enzymes with 3% weight of deer blood, and enzymolyze it at 50°C for 3 hours. By screening different enzymatic hydrolysis products, it is found that the enzymatic hydrolysis products of collagenase and bromelain can Significantly increased the swimming time of the mice (Table 1), indicating that the above two enzymatic hydrolysis products have anti-fatigue effects.

[0025] Table 1 Exhaustive swimming test table of rats with different enzymatic hydrolysis products

[0026]

[0027] *p<0.05, the difference is significant

[0028] In order to further improve the yield of enzymatic hydrolysis products and the anti-fatigue effect, the collagenase and bromelain were compounded, and the anti-fatigue effect was screened by changing the ratio of enzyme activity.

[0029] The results show that when the enzyme activity ...

Embodiment 2

[0045] Using the method determined in Example 1, using the above conditions to prepare deer blood peptides, determined by the Kjeldahl method, the peptide content is 87.65%, the peptides contain glycine, glutamic acid, arginine, lysine, tyrosine Amino acid, the total amino acid content (TAA) reached 75.58%, of which the essential amino acid (EAA) content was 36.51%, the EAA / TAA value was 49.13%, and the EAA / NEAA value was 98%; the first limiting amino acid was tryptophan.

[0046] Table 4: amino acid content of deer blood polypeptide (unit: %)

[0047]

[0048]

[0049] Exhaustive swimming test ( figure 1 ) results showed that the swimming time of mice given deer blood polypeptide was significantly enhanced (P<0.05), and physical fatigue could be better relieved at three dose levels. In particular, at the same concentration and dosage, the effect of using deer blood powder is significantly lower than that of deer blood polypeptide. Therefore, through the method determi...

Embodiment 3

[0053] Embodiment 3: the preparation of Lycium barbarum polysaccharide

[0054] Take 5 kg of medlars, wash them with tap water and pure water in turn, dry them, crush them, and pass them through a 15-mesh sieve. Add the crushed and sieved Lycium barbarum into the subcritical extraction device, and then add 25m 3of butane. After filling with nitrogen for 10 min, subcritical extraction was carried out. Extraction pressure 5MPa, extraction time 30min. The solvent was removed, and the fat-soluble components of Lycium barbarum and the residue of Lycium barbarum were collected. Add 5 times the amount of deionized water to the wolfberry slag, and cut it with a high-speed shear for 30 minutes to obtain the wolfberry soaking liquid. The shearing speed is 10,000 rpm. Transfer the above sheared Lycium barbarum soaking solution to the extraction tank, then add 7 times the volume of deionized water for extraction for 2 hours at an extraction temperature of 60°C; add 10 times the volume...

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Abstract

The invention provides a deer blood polypeptide. The prepared deer blood polypeptide and Chinese wolfberry polysaccharide are used as effective components to prepare a composition with an anti-fatigueeffect. The prepared composition has a synergistic interaction effect, and can be used for food with the anti-fatigue effect, functional food, food for special medical purposes and medicines. The deer blood polypeptide prepared by enzymolysis of deer blood with collagenase and bromelain is combined with Chinese wolfberry polysaccharide for use, and the anti-fatigue activity of the deer blood polypeptide-Chinese wolfberry polysaccharide composition is obviously higher than the anti-fatigue activity of single deer blood polypeptide or single Chinese wolfberry polysaccharide.

Description

technical field [0001] The invention belongs to the technical field of extraction and application of active substances, and in particular relates to a deer blood polypeptide and an anti-fatigue health product jointly prepared by using deer blood polypeptide and wolfberry polysaccharide. Background technique [0002] Fatigue refers to the inability of the body to start or continue a certain intensity of activity during physiological activities. In today's society, due to factors such as the accelerated pace of work and life and irregular work and rest, many people have been under great psychological pressure for a long time or are in a state of overtime and overloaded work. Dizziness, headache, loss of appetite, poor spirits, memory loss, etc. According to the survey of the World Health Organization, more than 35% of people in the world are in a state of fatigue, especially the fatigue state of middle-aged male population is as high as 60%-75%. Fatigue can cause the accumul...

Claims

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Application Information

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IPC IPC(8): A23J3/34A23J3/12A23J1/06A23L33/18A23L33/125
CPCA23J3/345A23J3/12A23J1/06A23L33/18A23L33/125A23V2002/00A23V2200/30A23V2250/55A23V2250/51
Inventor 邸多隆魏鉴腾裴栋王宁丽王晗刘建飞
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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