Anti-fatigue health-care product prepared from deer blood polypeptide and Chinese wolfberry polysaccharide
A deer blood polypeptide and anti-fatigue technology, applied in the direction of polysaccharide/gum-containing food ingredients, blood protein components, applications, etc., can solve problems such as poor palatability, difficult absorption and digestion, and getting angry
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Embodiment 1
[0023] Example 1 Screening of Deer Blood Polypeptide Preparation Method
[0024] Take 10kg of deer blood, after high-speed shearing and homogenization, add different enzymes with 3% weight of deer blood, and enzymolyze it at 50°C for 3 hours. By screening different enzymatic hydrolysis products, it is found that the enzymatic hydrolysis products of collagenase and bromelain can Significantly increased the swimming time of the mice (Table 1), indicating that the above two enzymatic hydrolysis products have anti-fatigue effects.
[0025] Table 1 Exhaustive swimming test table of rats with different enzymatic hydrolysis products
[0026]
[0027] *p<0.05, the difference is significant
[0028] In order to further improve the yield of enzymatic hydrolysis products and the anti-fatigue effect, the collagenase and bromelain were compounded, and the anti-fatigue effect was screened by changing the ratio of enzyme activity.
[0029] The results show that when the enzyme activity ...
Embodiment 2
[0045] Using the method determined in Example 1, using the above conditions to prepare deer blood peptides, determined by the Kjeldahl method, the peptide content is 87.65%, the peptides contain glycine, glutamic acid, arginine, lysine, tyrosine Amino acid, the total amino acid content (TAA) reached 75.58%, of which the essential amino acid (EAA) content was 36.51%, the EAA / TAA value was 49.13%, and the EAA / NEAA value was 98%; the first limiting amino acid was tryptophan.
[0046] Table 4: amino acid content of deer blood polypeptide (unit: %)
[0047]
[0048]
[0049] Exhaustive swimming test ( figure 1 ) results showed that the swimming time of mice given deer blood polypeptide was significantly enhanced (P<0.05), and physical fatigue could be better relieved at three dose levels. In particular, at the same concentration and dosage, the effect of using deer blood powder is significantly lower than that of deer blood polypeptide. Therefore, through the method determi...
Embodiment 3
[0053] Embodiment 3: the preparation of Lycium barbarum polysaccharide
[0054] Take 5 kg of medlars, wash them with tap water and pure water in turn, dry them, crush them, and pass them through a 15-mesh sieve. Add the crushed and sieved Lycium barbarum into the subcritical extraction device, and then add 25m 3of butane. After filling with nitrogen for 10 min, subcritical extraction was carried out. Extraction pressure 5MPa, extraction time 30min. The solvent was removed, and the fat-soluble components of Lycium barbarum and the residue of Lycium barbarum were collected. Add 5 times the amount of deionized water to the wolfberry slag, and cut it with a high-speed shear for 30 minutes to obtain the wolfberry soaking liquid. The shearing speed is 10,000 rpm. Transfer the above sheared Lycium barbarum soaking solution to the extraction tank, then add 7 times the volume of deionized water for extraction for 2 hours at an extraction temperature of 60°C; add 10 times the volume...
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