Multi-channel detection system for real-time nucleic acid fluorescence detection

Abstract

The invention discloses a multi-channel detection system for real-time nucleic acid fluorescence detection. The multi-channel detection system comprises a temperature control module, a hot cover, a filter wheel, a Y-shaped fluorescence detection optical fiber, a motion scanning module and a photoelectric detection module, wherein an exciting light LED emits exciting light, the exciting light is collimated by a collimating lens, the exciting light is filtered to form light waves with specific wavelengths by a exciting light filter, and the light waves pass through the Y-shaped fluorescence detection optical fiber, are focused by the lens and irradiate a sample in a test tube to excite a fluorescence signal, the fluorescence signal is focused on the Y-shaped fluorescence detection optical fiber through the lens, an invalid signal is filtered out through the emitted light filter, and a fluorescence signal is converted into an electric signal through a collimating lens focusing and photoelectric detection module. The system is advantaged in that a motor drives a filter wheel to rotate by a specific angle so as to switch a filter assembly with a specific wavelength to realize a multi-band detection function; the Y-shaped fluorescence detection optical fiber is driven by a motion scanning module to move so as to realize detection of multiple test tube hole sites.

Description

technical field

[0001] The invention belongs to the field of molecular diagnosis and relates to a multi-channel detection system for real-time nucleic acid fluorescence detection. Background technique

[0002] Multiplex PCR (multiplex PCR), also known as multiple primer PCR or composite PCR, is a PCR reaction in which two or more pairs of primers are added to the same PCR reaction system to simultaneously amplify multiple nucleic acid fragments. Its reaction principle, reaction reagents and operation The process is the same as general PCR. Multiplex PCR (Multiplex PCR), that is, using the specificity of primers, fluorescent probes and target DNA, adding multiple pairs of primers and multiple fluorescent probes for multiple target DNA in the reaction system, and detecting it by a real-time fluorescent quantitative PCR Multiple target DNA. This method overcomes the disadvantages of cumbersome operation, difficult quantification, and easy contamination of ordinary PCR, improv...

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