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A kind of detection primer, detection kit, detection method and application of chicken Columbia plumage genotype

A technology for detecting kits and detecting primers, which is applied in the field of animal genetics and breeding, and can solve problems such as the absence of better methods for detecting chickens

Active Publication Date: 2021-10-22
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chicken feather color is a quality trait, which is controlled by many genes, and its inheritance is also a complicated process. At present, there is no good method for detecting the genotype of chicken Columbia plumage

Method used

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  • A kind of detection primer, detection kit, detection method and application of chicken Columbia plumage genotype
  • A kind of detection primer, detection kit, detection method and application of chicken Columbia plumage genotype
  • A kind of detection primer, detection kit, detection method and application of chicken Columbia plumage genotype

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Experimental program
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Effect test

Embodiment 1

[0040] The nucleotide sequence of the detection primer of the chicken Columbia feather genotype in the present embodiment is as follows:

[0041] Upstream primer P-F: 5'-CGTATACACAGCCTGGCCTAA-3';

[0042] Downstream primer P-R: 5'-ATTCCTGTGTGTGGTGGAGTG-3'.

[0043] Example 1 of the Detection Kit for Chicken Columbia Feather Genotype

[0044] In this embodiment, the detection kit for chicken Columbia feather genotype includes: detection primers, 2×Taq PCRmasterMix, ddH 2 O. Among them, 2×Taq PCR masterMix is ​​provided by Kangwei Century Biotechnology Co., Ltd., product number Lot01036 / 30146.

[0045] The nucleotide sequences of the detection primers are as follows:

[0046]Upstream primer P-F: 5'-CGTATACACAGCCTGGCCTAA-3';

[0047] Downstream primer P-R: 5'-ATTCCTGTGTGTGGTGGAGTG-3'.

[0048] Example 1 of the Detection Method of Chicken Columbia Feather Genotype

[0049] The detection method of chicken Columbia plumage genotype in the present embodiment may further compri...

Embodiment 2

[0061] The technical flow diagram of the detection method of the chicken Columbia plumage genotype of the present embodiment is as follows figure 1 As shown, it mainly includes the following steps:

[0062] 1) Preparation and storage of samples

[0063] 15 Jute Yu Gushi roosters and 150 H-series hens of Columbia Yuyufen No. figure 2 shown), including 39 F1 Colombian cocks and 38 jute hens; F2 chickens are the offspring of 2 F1 Colombian cocks and 20 F1 F1 jute hens, including 181 Columbian cocks 212 Colombian hens, 192 jute roosters and 186 jute hens; the F3 generation A group selected F2 Colombian roosters and Columbian hens to cross, and produced 36 Columbian roosters and Columbian hens 18 chickens and 22 hens with jute feathers; Group B selected F2 Colombian cocks and hens with jute feathers to cross, and produced 15 Columbia cocks, 16 Colombian hens, 15 jute cocks and 14 jute hens Only. Take the isolated tissues, organs or blood of the chicken to be tested, extract th...

Embodiment 3

[0086] The detection method of the chicken Columbia feather genotype of the present embodiment mainly comprises the following steps:

[0087] 1) Preparation and storage of samples

[0088] 96 Gushi chickens (jute feathers), 96 Lushi chickens (jute feathers), 96 Changshun chicken green-shell layer hens (yellow feathers), 96 F2 resource groups of Gushi chickens and Anka chickens (GxA F2, yellow feather), 96 recessive white-feather chickens (white feather), 96 Xichuan silky chickens (white feather), 96 AA chickens (white feather), 96 Dongxiang chicken green-shell layer hens (black feather). Take the isolated tissues, organs or blood of the chicken to be tested, extract the DNA with the proteinase K method, and store the DNA samples in a 4°C refrigerator for later use.

[0089] Step 2), step 3) and step 4) are the same as Example 2 of the detection method of chicken Columbia feather genotype.

[0090] 5) Test results

[0091] Test results such as Figure 4 shown in . The typi...

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Abstract

The invention relates to a detection primer, a detection kit, a detection method and an application of chicken Columbia feather genotype, and belongs to the technical field of animal genetics and breeding. found in the present invention RAI14 The insertion of the 33-95th 63bp base sequence in the upstream region sequence of the gene is closely related to the chicken Colombian plumage traits, so the present invention utilizes RAI14 According to the characteristics of the upstream region of the gene, a PCR genotyping method for the insertion was established, and then the Columbia feather chicken gene could be typed. The detection method of chicken Columbia feather genotype provided by the present invention is simple in operation, fast in detection and accurate in results, which is conducive to more effective and rapid selection of Columbia feather traits in breeding practice, speeds up breeding progress, and saves breeding costs. Breeding for traits of feather chickens provides a quick molecular breeding method.

Description

technical field [0001] The invention relates to a detection primer, a detection kit, a detection method and an application of chicken Columbia feather genotype, and belongs to the technical field of animal genetics and breeding. Background technique [0002] The feather color of chickens is very rich, which is one of the important breed characteristics of chickens, and is also an important selection index in chicken breeding work. The shallow reed feathers of chickens are also called Colombian feathers. The specific manifestations are: the main body of the feathers is basically white feathers, and the ends of the feathers on the neck, wings and tail have black horizontal spots in the shape of horizontal stripes and shallow reeds. It is sex-linked Colombian feathers. Sex-linked Columbia plumage has gained more attention and research because of its distinctive characteristics and sex-linked inheritance. [0003] As a trait, Colombian feathers are shown in the Yufen 1 H line, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 康相涛李转见田亚东刘丹丽韩瑞丽孙桂荣王鑫磊王永才李国喜闫峰宾蒋瑞瑞李红李文婷刘小军王彦彬
Owner HENAN AGRICULTURAL UNIVERSITY
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