A nano-microcapsule suitable for carrying Scleroderma sichuanensis and its preparation method
A technology of nano-microcapsules and scleroderma, applied in botany equipment and methods, chemicals for biological control, pesticides, etc., can solve the problems of limited use of biological control microcapsules and limited effect of biological control , to achieve the effect of improving the adhesion effect, completely killing the bacteria, and enhancing the adhesion
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Embodiment 1
[0030] A preferred embodiment of the present invention provides a method for preparing nanometer microcapsules suitable for Scleroderma sichuanensis, comprising the following steps:
[0031] Add chitosan to the shell solution, then mix it evenly with the core solution at 1000r / min, then add the mixture dropwise to the edible oil until a W / O emulsion is formed, then add 1wt% sodium chloride cross-linking agent Cross-linked and solidified, cleaned, dried, and obtained; wherein, the shell layer solution is a sodium alginate solution with a concentration of 1% by weight; the core layer solution is a sodium alginate solution with a concentration of 1×10 7 cfu / mL Beauveria bassiana spore powder suspension; Chitosan solution obtained by dissolving chitosan in 0.3wt% dilute acetic acid solution; Quality of sodium alginate solution, Beauveria bassiana spore powder suspension and chitosan solution The ratio is 30:70:1.
Embodiment 2
[0033] Only the concentration of sodium alginate solution is 3wt%, the rotating speed is 500r / min, and the concentration of sodium chloride is 3wt%; the others are the same as in Example 1.
Embodiment 3
[0035] Only the concentration of sodium alginate solution is 5wt%, the rotating speed is 1500r / min, and the concentration of sodium chloride is 5wt%; the others are the same as in Example 1.
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