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Sealed type high-flux high-efficiency full-automatic biosynthesis device

A biosynthesis, high-throughput technology, applied in biochemical cleaning devices, enzymology/microbiology devices, bioreactor/fermenter combinations, etc. It takes a long time to achieve the effect of improving synthesis efficiency, reducing single operation time, improving synthesis efficiency and synthesis quality

Pending Publication Date: 2020-12-11
DONGXUAN GENE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, adding samples to a 384-well plate is a very large task. In the prior art, there is no sample adding device that can match the high-density sample addition of a 384-well plate, resulting in low sample addition efficiency. , it takes a long time to synthesize
During the synthesis process, the reagents loaded on the synthesis plate need to be in a sealed environment according to the requirements of the synthesis conditions, and also need to be filled with an inert gas for protection. At the same time, all are manually operated, the workload is very large, the efficiency is extremely low, the sample size control accuracy is poor, and the synthesis conditions are unstable, resulting in poor synthesis efficiency.

Method used

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  • Sealed type high-flux high-efficiency full-automatic biosynthesis device
  • Sealed type high-flux high-efficiency full-automatic biosynthesis device
  • Sealed type high-flux high-efficiency full-automatic biosynthesis device

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Embodiment

[0037] Please refer to Figure 1-Figure 13 , the present embodiment is a sealed high-throughput and high-efficiency fully automatic biosynthesis device 100, which includes a moving plate 1 for horizontal linear motion, a number of synthesis chamber units fixed below the moving plate 1 and forming an upper opening 11 on the moving plate 1 2. The sealing plate assembly 3 located above the moving plate 1 and forms a sealed space with the moving plate 1 by moving up and down, the sample adding unit 4 fixed on the sealing plate assembly 3, and the sealing upper opening fixed on the sealing plate assembly 3 There are several pressurized sealing units 5 in 11, the moving plate 1 is driven by a driving member 6 and can move between the position of the sample adding unit 4 and the position of the independent pressurized sealing unit 5 .

[0038]This embodiment also includes a support 7, on which a set of supporting rollers 71 is arranged, and the moving plate 1 is erected on the set of...

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Abstract

The invention discloses a sealed type high-flux high-efficiency full-automatic biosynthesis device. The sealed type high-flux high-efficiency full-automatic biosynthesis device comprises a moving plate, several synthesis cavity units, a sealing plate assembly, a sample adding unit and several pressurizing sealing units; the moving plate moves horizontally and linearly; the several synthesis cavityunits are fixed below the moving plate and form an upper opening in the moving plate; the sealing plate assembly is positioned above the moving plate and forms a sealed space with the moving plate through up-down movement; the sample adding unit is fixed on the sealing plate assembly; the several pressurizing sealing units are fixed on the sealing plate assembly and seal the upper opening; and the movable plate is driven by a driving part to move among the position of the sample adding unit and the positions of the pressurizing sealing units. According to the sealed type high-flux high-efficiency full-automatic biosynthesis device in the invention, automatic addition of the sample amount in the synthesis reaction process of a large batch of ultra-micro biological samples is realized; primers are automatically transferred into a sealed reaction cabin; furthermore, automatic pressurization and pressure maintaining are carried out; the pressure and synthesis speed required by synthesis are precisely controlled; and the biosynthesis efficiency is greatly improved.

Description

【Technical field】 [0001] The invention belongs to the technical field of biosynthesis devices, in particular to a sealed high-throughput, high-efficiency, fully automatic biosynthesis device. 【Background technique】 [0002] At present, DNA synthesis columns often use single-column tubes or 96-well filter plates. Existing single-column tubes have many specifications and large volumes, which are not conducive to batch, large-scale, and miniaturized operations. For trace, ultra-micro, and large-scale DNA Synthesis, single-column tube is difficult to achieve; the existing 96-well filter plate mainly has two specifications of 1.5 and 2ml, and can complete the synthesis of 96 DNA samples at the same time, which is faster than a single tube for batch DNA synthesis There are many, but in terms of the sample size processed, it is still unable to meet the batch and large-scale synthesis of trace and ultra-trace DNA. Therefore, a 384-well plate has been developed on the market, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N15/10
CPCC12N15/10
Inventor 周郭丹玉吕永维T·德米特田敬东
Owner DONGXUAN GENE TECH CO LTD
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