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Pigeon paramyxo virus type 1 PPMV-1/BJ-C strain and application thereof

A technology of PPMV-1 and BJ-C, applied in the direction of virus/bacteriophage, virus, antiviral agent, etc., can solve the problems of low protection efficiency and unsatisfactory immune effect of pigeons, and achieve stable potency, fast onset, and immunity Original good effect

Inactive Publication Date: 2020-12-11
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The study found that the genotype of pigeon plague virus is quite different from the genotype of the existing chicken NDV-related vaccine strains in my country, the immune effect on pigeons is not ideal, and the protection efficiency is low
Currently, there is no vaccine on the market that specifically prevents pigeon plague

Method used

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  • Pigeon paramyxo virus type 1 PPMV-1/BJ-C strain and application thereof
  • Pigeon paramyxo virus type 1 PPMV-1/BJ-C strain and application thereof
  • Pigeon paramyxo virus type 1 PPMV-1/BJ-C strain and application thereof

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Embodiment 1

[0173] The acquisition of embodiment 1 pigeon plague virus attenuated strain PPMV-1 / BJ-C

[0174] Through the epidemiological investigation of pigeon plague, a virulent strain of pigeon plague virus PPMV-1 / BJ was screened out. The attenuated strain PPMV-1 / BJ-C strain (the deposit number is CGMCC No. 19989) was constructed with the help of infectious cloning platform, and the inactivated vaccine of pigeon plague virus (PPMV-1 / BJ-C strain) was studied. Using SPF chicken embryo culture, the virus content was determined and inactivated by formaldehyde solution. Then, quality control indicators such as safety, immune protection and stability were tested. The results showed that the vaccine was safe and effective, and played a good protective role against the current circulating strains. The specific method is as follows:

[0175] 1. Chicken embryos for production

[0176] The SPF chicken embryos used were all provided by Beijing Boehringer Ingelheim Weitong Biotechnology Co., Lt...

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Abstract

The invention provides a pigeon paramyxo virus type 1 PPMV-1 / BJ-C strain and application thereof. The attenuated strain PPMV-1 / BJ-C is constructed by mutating an F protein cleavage site of the attenuated strain PPMV-1 / BJ-C into a La Sota strain corresponding site on the basis of a pigeon plague virus virulent strain PPMV-1 / BJ. PPMV-1 / BJ-C has the advantages of good immunogenicity, good culture characteristics, stable titer and the like, the pigeon plague virus PPMV-1 / BJ-C strain is inoculated to 9-11-day-old SPF chick embryos through an allantoic cavity, allantoic fluid of infected chick embryos is harvested and inactivated with diethyleneimine, and then an aluminum hydroxide adjuvant is added to prepare a pigeon plague virus inactivated vaccine. The inactivated vaccine prepared from the PPMV-1 / BJ-C strain can generate a relatively high antibody, can prevent ND caused by pigeon plague virus, and the vaccine has the advantages of safety, quick response, lasting immune period and the like, and has wide application prospects.

Description

technical field [0001] The invention relates to the field of biological products and preventive veterinary medicine, in particular to pigeon paramyxovirus type 1 PPMV-1 / BJ-C strain and application thereof. Background technique [0002] Pigeon plague, also known as pigeon Newcastle disease (Newcastle Disease, ND), is caused by pigeon type I paramyxovirus (Pigeon Paramyxo Virus I, PPMV-I) with diarrhea High-contact infectious diseases with neurological symptoms as the main symptoms. PPMV-I is an antigenic variant of Newcastle Disease Virus (NDV). Pigeon plague has always been an important infectious disease affecting the development of my country's pigeon industry. The study found that the genotype of pigeon plague virus is quite different from the genotype of the existing chicken NDV-related vaccine strains in my country, the immune effect on pigeons is not ideal, and the protection efficiency is low. Currently, there is no vaccine on the market that specifically prevents ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N7/04G01N33/569A61K39/17A61P31/14C12R1/93
CPCC12N7/00G01N33/56983A61K39/12A61P31/14C12N2760/18121C12N2760/18162G01N2333/125C12N2760/18134A61K2039/552A61K2039/5252
Inventor 崔尚金梁琳梁瑞英韩坤
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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