Nucleic acid reagent reaction device

Abstract

The invention discloses a nucleic acid reagent reaction device, which comprises a sample transfer unit, a vacuum sealed reaction unit and a vacuum sealed sample storage unit, the sample transfer unitcomprises an accommodating pipe, a liquid suction / discharge module communicated with the accommodating pipe, a sample injection module and a sample adding module; valves are arranged on the communicating pipelines of the sample injection module, the sample adding module and the accommodating pipe; the sample injection module extends into the sample storage unit and is hermetically connected with the sample storage unit; the sample adding module extends into the reaction unit and is hermetically connected with the reaction unit; the liquid suction / discharge module is used for sucking the samplein the sample storage unit into the accommodating pipe and discharging the sample in the accommodating pipe to the reaction unit. The method solves the problem of rapid nucleic acid detection on sitein a non-laboratory environment, can be applied in multiple scenes, can move the nucleic acid detection tailored to clinical application and basic level, and is a nucleic acid detection iteration technology without laboratory environment, with shorter detection time, more convenient detection means, higher accuracy and better security.

Description

technical field [0001] The application relates to a nucleic acid reagent reaction device, which belongs to the technical field of nucleic acid detection. Background technique [0002] Polymerase chain reaction technology (hereinafter referred to as PCR technology) is a technology for rapidly amplifying DNA in vitro, which usually includes multiple cycles, and each cycle includes three steps of denaturation, annealing and extension, and ideally , after each cycle, the number of target nucleic acid molecules is doubled. Generally speaking, after 30-40 cycles, the number of target nucleic acid molecules can be amplified to the original nearly 10. 9 times. Therefore, PCR is the most effective method to obtain a large amount of target DNA fragments in vitro, and the obtained nucleic acid molecules can be used for further analysis and inspection. [0003] However, the high sensitivity of PCR is always accompanied by a drawback: it is extremely easy to be contaminated. Contamin...

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Problems solved by technology

[0005] The purpose of this application is to provide a nucleic acid reagent reaction device to solve the technical problem that the nucleic acid reaction device in the prior art is not suitable for on-site rapid detection of nucleic acid

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