A kind of Raman detection kit and Raman detection method of organophosphorus

A detection kit and detection method technology, applied in Raman scattering, measurement device, material excitation analysis, etc., can solve the problems of low detection sensitivity, long time, low accuracy, etc., achieve simple components, improve detection sensitivity, low cost effect

Active Publication Date: 2021-09-24
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above methods generally have problems such as long time-consuming, low accuracy, and low detection sensitivity.

Method used

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  • A kind of Raman detection kit and Raman detection method of organophosphorus
  • A kind of Raman detection kit and Raman detection method of organophosphorus
  • A kind of Raman detection kit and Raman detection method of organophosphorus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] In this example, the preparation of the Raman detection kit for organophosphorus and the Raman detection of the analytes were carried out at room temperature.

[0040] Prepare the sample to be tested: prepare a solution with a concentration of 1000mg / LDMMP as the sample to be tested.

[0041] For the preparation of Au sol, add chloroauric acid solution into 50mL water, heat to boil, then add sodium citrate solution, continue heating for 30min, and cool to room temperature, wherein the molar ratio of chloroauric acid to sodium citrate is (4-6): 3. Au sol with a particle size of 30-100nm is obtained.

[0042] For the preparation of core-shell nanoparticle sol, disperse the obtained Au sol in 50mL ultrapure water, add about 3mL of sodium citrate with a mass fraction of 1% and ascorbic acid with a mass fraction of 1%, mix well and then add 10mL of concentration dropwise 20mmol / L silver nitrate solution, stirred for a certain period of time, centrifuged, washed, and concent...

Embodiment 2

[0047]In this example, the preparation of the Raman detection kit for organophosphorus and the Raman detection of the analytes were carried out at room temperature.

[0048] Prepare the sample to be tested: Prepare the mother solution with a concentration of 1000mg / LDMMP, and dilute them respectively to obtain DMMP standard solutions with concentrations of 100mg / L, 10mg / L, 1mg / L, 100μg / L, 50μg / L and 10μg / L as the samples to be tested.

[0049] For the preparation of Au sol, take 1% chloroauric acid solution and add it to 50mL water, heat to boil, then add 2mL sodium citrate solution with a concentration of 1%, continue heating for 30min, and cool to room temperature to obtain a particle size of 30 - 100nm Au sol.

[0050] For the preparation of core-shell nanoparticle sol, disperse the obtained Au sol in 50mL ultrapure water, add about 3mL of sodium citrate with a mass fraction of 1% and ascorbic acid with a mass fraction of 1%, mix well and then add 10mL of concentration drop...

Embodiment 3

[0055] Prepare the sample to be tested: prepare a DMMP mother solution with a concentration of 1000 mg / L, and dilute it to obtain a DMMP standard solution with a concentration of 100 mg / L as the sample to be tested.

[0056] In Example 3, the same process conditions as in Example 2 of the present invention were used to detect the 100 mg / L DMMP sample to be tested. The difference was that Example 3 was carried out at 10°C and 50°C respectively.

[0057] Figure 5 is the Raman detection spectrum of the solution to be tested under different temperature conditions. The results show that the organophosphorus Raman detection kit and the Raman detection method of the present invention can obtain relatively stable and strong signals in the face of large fluctuations in the temperature of the test environment.

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Abstract

The invention provides a Raman detection kit for organophosphorus, which is characterized in that it includes a SERS enhanced chip and an enhanced auxiliary agent; the SERS enhanced chip includes a substrate and core-shell structured nanoparticles, and the core-shell structured nanoparticles are attached to On the substrate, the enhancer is KI, and the second aspect of the present invention provides a Raman detection method using an organophosphate detection kit. In the present invention, nanoparticles with a core-shell structure are used as a reinforcement matrix to coat the surface of the substrate to prepare a SERS enhancement chip, and a certain amount of enhancement additive is added to the sample to be tested to improve the detection sensitivity of organophosphorus poisons, and can detect organic phosphorus poisons. The concentration of phosphorus is as low as 10 μg / L, breaking through the limitation of low sensitivity of the portable Raman spectrometer, and the Raman detection kit and Raman detection method of the present invention can obtain relatively stable and a stronger signal.

Description

Technical field: [0001] The invention relates to the field of rapid Raman detection, in particular to a Raman detection kit and a Raman detection method for organophosphorus. Background technique: [0002] Organophosphorus poisons include organophosphorus pesticides and nerve agents (NAs), both of which belong to organophosphorus compounds. Dimethyl methyl phosphate (DMMP) is currently one of the most effective additive organic phosphorus flame retardants in the world. It can be miscible with water and various organic solvents, and has the effect of reducing viscosity and surface adhesion. The molecular structure of DMMP is similar to the chemical warfare agent sarin (isopropyl meflodronate, GB), and it is often used as a sarin simulant for research. There are many methods for DMMP testing, including spectroscopic spectroscopy, gas chromatography, vibrational spectroscopy, quartz crystal microbalance (QCM) sensors, surface acoustic wave (SAW) sensors, etc. However, the abo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 李剑锋温宝英
Owner XIAMEN UNIV
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