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Application of pathogenic fungi, method for preventing and controlling pests and insecticide

A technology of pathogenic fungi and insecticides, applied in the direction of insecticides, applications, biocides, etc., can solve the problems of aphids rampant again, affecting human health, and aphids developing drug resistance

Active Publication Date: 2021-01-12
QIQIHAR BRANCH OF HEILONGJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical pesticides are mainly used to control corn aphids, which not only affect human health and cause ecological environment pollution, but also cause aphids to develop resistance to pesticides after long-term application, leading to rampant aphids again, making it difficult to control

Method used

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  • Application of pathogenic fungi, method for preventing and controlling pests and insecticide
  • Application of pathogenic fungi, method for preventing and controlling pests and insecticide
  • Application of pathogenic fungi, method for preventing and controlling pests and insecticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, isolation and identification of pathogenic fungi

[0032] (1) Isolation of bacterial strain SGSF001 and bacterial strain SGSF221

[0033]The strains were isolated from the understory litter in Daxing'an Mountains as the source of isolation. Firstly, the collected air-dried litter samples were pulverized into particles, and filtered with a filter screen to obtain particles of litter samples with a particle size of 100-200 μm. Then, using the plate dilution method, the granular sample was mixed with sterile water at a mass ratio of 1:100 to prepare a particle suspension. Then use a pipette gun to draw 50 μL, 100 μL, 150 μL, 200 μL and 250 μL and apply them on the PDA medium plate respectively, spread it with a coating rod until the particles are evenly dispersed, place it at room temperature and observe it every 12 hours. Colonies were picked to PDA plates for purification. Two strains of fungi were obtained after purification and culture, which were numb...

Embodiment 2

[0046] Embodiment 2, the biological characteristics of two strains of pathogenic fungi

[0047] 1. Experimental method

[0048] (1) Determination of strain growth rate and spore production at different temperatures

[0049] Use a hole puncher to punch holes in the colonies of strain SGSF001 and strain SGSF221, and then transfer them to PDA medium, and place them in incubators at 20°C, 25°C, 30°C, and 35°C, respectively, and repeat for each treatment. Three times, the colony growth diameter was regularly measured every day for a total of 14 days. At the end of the measurement experiment, 5 mL of sterilized 1% Tween 80 aqueous solution was added to the plate, the surface spores were scraped off, and after the excess mycelium and agar were filtered out with sterile cotton, the amount of spore production was determined using a hemocytometer.

[0050] (2) Determination of strain growth rate and spore production under different pH

[0051] The colonies of strain SGSF001 and strai...

Embodiment 3

[0069] Embodiment 3, two strains of pathogenic fungi insect-resistant activity assay

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PUM

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Abstract

The invention belongs to the field of biological prevention and control, and provides application of pathogenic fungi, a method for preventing and controlling pests and an insecticide. In order to solve the problems of drug residue, poor selectivity, short lasting period and the like of chemical pesticide pest control, two fungi are obtained through separation, the biological characteristics and the insect-resistant activity of the two fungi are measured respectively, and experiments prove that the pathogenic fungi have good insect-resistant activity and wide infection range, and can be used as biocontrol strains. The invention provides an alternative strain for biological control of pests.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to the application of a pathogenic fungus and an insecticide. Background technique [0002] Due to the serious problem of chemical pesticide residues, the use of biological means to control pests has become an important choice. The use of entomopathogenic bacteria (such as Beauveria bassiana and Metarhizium anisopliae) to control pests is considered to be an effective green control method. However, some pests, such as Monolepta hieroglyphica, lack green control methods, and there is no report on the use of entomopathogens to control Monolepta hieroglyphica. Metarhizium anisopliae is widely used in pest control as an entomopathogen, and has good control effects. Among them, Metarhizium anisopliae is the most widely used due to its strong virulence, wide infection range, and long-lasting effect. [0003] Monolepta hieroglyphica belongs to Coleoptera Chrysalis family. It...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/30A01P7/04
CPCA01N63/30
Inventor 郑旭张超杨慧莹赵秀梅徐利剑高思禹张李香宋福强
Owner QIQIHAR BRANCH OF HEILONGJIANG ACADEMY OF AGRI SCI
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