PH responding dual-drug-loading system, preparation method and application thereof

A dual drug-loading and system technology, applied in the field of material synthesis and biomedicine

Active Publication Date: 2021-01-15
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, simple drug delivery systems

Method used

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  • PH responding dual-drug-loading system, preparation method and application thereof
  • PH responding dual-drug-loading system, preparation method and application thereof
  • PH responding dual-drug-loading system, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A preparation method of a pH-responsive dual-loaded drug system comprises the following steps:

[0037] (1) Accurately weigh 5mg of methotrexate (molecular weight 454.44) and dissolve it in 50mL of deionized water, ultrasonically disperse for 3 hours, after the solution turns yellow and transparent, add 0.87g sodium dodecylbenzenesulfonate (molecular weight 348.48) and 0.555g calcium chloride (molecular weight 110.98), magnetically stirred for 3 hours to make the mixture uniform, then added 0.529g sodium carbonate (molecular weight 105.99), continued magnetic stirring for 3 hours, centrifuged and washed the solid product, and dried at 60°C for 6 hours. That is methotrexate / calcium carbonate nano-microspheres.

[0038] (2) Accurately weigh 6mg of aspirin (molecular weight 108.16) and dissolve it in 20mL of deionized water, ultrasonically disperse for 3 hours, after the aspirin is completely dissolved, add 0.1g sodium carboxymethylcellulose and 0.1g sodium alginate, and s...

Embodiment 2

[0041] The release behavior of methotrexate from methotrexate / calcium carbonate nanospheres at different pH values ​​includes the following steps:

[0042] The preparation process of methotrexate / calcium carbonate nano-microspheres is the same as that of Example 1.

[0043] (1) Weigh six parts of methotrexate / calcium carbonate with a mass of 800 mg, place them in six dialysis bags, and place the dialysis bags in 50 mL of phosphate buffer solution with different pH; o Under the condition of C, magnetic stirring is carried out to release the methotrexate; the pH values ​​of the phosphate buffer solution are 1.2, 5.8, 6.0, 6.5, 7.0 and 7.4 respectively, and the release is performed for 24 hours.

[0044] (2) Take out 3mL of solution at regular intervals, measure the amount of methotrexate released, and supplement 3mL of fresh phosphate buffer solution at the same time; measure the concentration of methotrexate at 302nm using an ultraviolet spectrophotometer The intensity of the ...

Embodiment 3

[0046] The release behavior of methotrexate and aspirin from methotrexate / calcium carbonate / aspirin / carboxymethylcellulose sodium / calcium ion / sodium alginate dual-loaded drug system at different pH values ​​includes the following steps:

[0047] The preparation process of the methotrexate / calcium carbonate / aspirin / sodium carboxymethyl cellulose / calcium ion / sodium alginate dual-loaded system is the same as that in Example 1.

[0048] (1) Weigh three parts of 800mg methotrexate / calcium carbonate / aspirin / carboxymethylcellulose sodium / calcium ion / sodium alginate dual drug system, put them in three dialysis bags, and dialyze The bags were placed in 50 mL of phosphate buffer solution with different pH; the two drugs were released by magnetic stirring at a temperature of 37°C; the pH values ​​of the phosphate buffer solution were 1.2, 5.8 and 7.4, and the release time Hour.

[0049] (2) Take out 3mL of the solution at regular intervals, measure the amount of the two drugs released, ...

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Abstract

The invention discloses a pH responding dual-drug-loading system, a preparation method and application thereof. Combining calcium carbonate nano microspheres, sodium alginate and sodium carboxymethylcellulose together to prepare a pH responding dual-drug-loading system. The preparation method comprises the following steps: preparing anti-cancer drug/calcium carbonate nano microspheres, and preparing an anti-cancer drug/calcium carbonate/analgesic drug/sodium carboxymethylcellulose/calcium ion/sodium alginate dual-drug-loading system. The pH responding dual-drug-loading system prepared by the invention can simultaneously load an anti-cancer drug and an analgesic drug, and realizes independent release of the two drugs in different pH environments in vivo. In the dual-drug-loading system, a short-term release of the analgesic drug aspirin can be achieved, and a long-term and continuous release of the anti-cancer drug methotrexate can be achieved.

Description

technical field [0001] The invention belongs to the technical field of material synthesis and biomedicine, and in particular relates to a pH-responsive dual-loaded drug system and its preparation method and application. Background technique [0002] In recent years, the combination therapy of drugs with different efficacy has been effective in the treatment of diseases and tissue regeneration. In order to achieve the best curative effect, different drugs should be used during the course of treatment according to the optimal dosage of different drugs and different treatment cycles. One of the major challenges of combined therapy is to achieve independent control of the release behavior of each drug. However, simple drug delivery systems cannot meet the needs of this therapy. Therefore, it is very necessary to develop drug sustained-release systems that can control the independent release of each drug. Contents of the invention [0003] Purpose: In order to overcome the d...

Claims

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Application Information

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IPC IPC(8): A61K9/19A61K47/02A61K47/36A61K47/38A61K31/616A61K31/519A61P35/00
CPCA61K9/19A61K47/02A61K47/36A61K47/38A61K31/616A61K31/519A61P35/00A61K2300/00
Inventor 孔泳盛焱山康静吴大同周海逢蔡文蓉
Owner CHANGZHOU UNIV
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