Acinetobacter database construction method based on mass spectrometry and acinetobacter database

A construction method and database technology, applied in the field of Acinetobacter database, can solve the problems of incomplete strain information and low accuracy, and achieve the effect of comprehensive database, high accuracy, and not easy to miss false positives
CN112331267AInactive Publication Date: 2021-02-05ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2021-02-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an acinetobacter database construction method based on mass spectrometry and an acinetobacter database; and the acinetobacter database construction method comprises the following steps: performing separating to obtain an acinetobacter colony, and performing enrichment cryopreservation to ensure that the purity of the obtained strain is 100%; carrying out NGS sequencing on the strain to obtain a sequence, assembling the sequence, carrying out ANI calculation on the assembled sequence and an NCBI database genome of acinetobacter pairwise, and taking a result with the highest matching degree in calculation results as a species identification result; respectively extracting the strains obtained in the step 1 by adopting a direct coating method and / or an extraction method, and collecting spectrums after extraction to obtain a biological polypeptide fingerprint standard spectrum; opening micro type post-processing software by double click, creating a laboratory acinetobacter self-built library, building nodes according to the kingdom, phylum, class, order, family, genus and species sequence, selecting new acinetobacter data needing to be added, selecting high-quality map to build data, unifying the determined biological polypeptide fingerprint standard map and the name of the strain obtained by NGS sequencing, and adding into the corresponding nodes; and completing the library construction of the strain.
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Description

technical field

[0001] The invention relates to the technical field of mass spectrometry detection, in particular to a method for constructing an Acinetobacter database based on mass spectrometry and the Acinetobacter database. Background technique

[0002] Acinetobacter bacteria belong to Proteobacteria, Gamma Proteobacteria, Pseudomonas, Moraxellaceae bacteria, are important conditional pathogens causing nosocomial infections, mainly threatening patients in the intensive care unit, causing Common infections include bloodstream infections, ventilator-associated pneumonia, urinary tract infections, meningitis, and wound infections, which are associated with high morbidity and mortality in infected patients. Infection outbreaks in many hospitals are caused by Acinetobacter, due to its high resistance in the environment and resistance to antimicrobials, making the infection difficult to control and treat, and leading to prolonged hospital stays and high hospital costs . [0...

Claims

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