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Preparation method of animal peripheral blood erythrocytes

A technology for red blood cells and animals, applied in the field of red blood cell preparation, can solve the problems of short effective storage time of red blood cells, waste of mouse red blood cells, low extraction rate, etc., and achieve the effects of prolonging effective storage time, long storage time and easy operation.

Pending Publication Date: 2021-02-12
JILIN GETEIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction rate is low, time-consuming and costly
The effective storage time of the prepared red blood cells is short, resulting in the need for frequent preparation and waste of mouse red blood cells

Method used

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  • Preparation method of animal peripheral blood erythrocytes
  • Preparation method of animal peripheral blood erythrocytes
  • Preparation method of animal peripheral blood erythrocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The steps of extracting bovine whole blood erythrocytes according to the present invention are:

[0033] (1) Preparation: Prepare fresh whole blood of fresh animals (within 0.5-2 hours of slaughter);

[0034] (2) Pretreatment: Centrifuge the fresh whole blood of fresh animals at 200-800g for 20-40min and filter it with a 0.22-0.45nm filter membrane. Add 0.01-0.5% antioxidant, stand at 0-10°C for 8-12h;

[0035] (3) Rough extraction: centrifuge the sample in (2) at 200-800g for 20-40min. Stratification occurs after centrifugation, remove the top 1-3.0ml sample, and carefully absorb 2-4.5ml of the remaining sample. The aspirated sample is then mixed. Repeat the above operation 2-5 times;

[0036] (4) Blocking process: Take the above (3) sample in a centrifuge tube and add 2-5 times the volume of cell blocking agent. The temperature is 4-10°C, the centrifugal force is 400-900g, and the centrifugal force is 15-45min. Remove the 2.5-4.5ml sample above the centrifuge tu...

Embodiment 2

[0047] (1) Preparation: Prepare fresh whole blood of fresh animals (within 0.5-2 hours of slaughter);

[0048] (2) Pretreatment: Centrifuge fresh whole blood of fresh animals at 200-800g for 20-40min and then filter with a 0.22-0.45nm filter membrane. Add 0.01-0.5% antioxidant, stand at 0-10°C for 8-12h;

[0049] (3) Rough extraction: centrifuge the sample in (2) at 200-800g for 20-40min. Stratification occurs after centrifugation, remove the top 1-3.0ml sample, and carefully absorb 2-4.5ml of the remaining sample. The aspirated sample is then mixed. Repeat the above operation 2-5 times;

[0050] (4) Blocking process: Take the above (3) sample in a centrifuge tube and add 2-5 times the volume of cell blocking agent. The temperature is 4-10°C, the centrifugal force is 400-900g, and the centrifugal force is 15-45min. Remove the 2.5-4.5ml sample above the centrifuge tube, and carefully pipette the remaining 2-3ml sample. Repeat the above operation 1-3 times;

[0051] (5) D...

Embodiment 3

[0061] (1) Preparation: Prepare fresh whole blood of fresh animals (within 0.5-2 hours of slaughter);

[0062] (2) Pretreatment: Centrifuge fresh whole blood of fresh animals at 200-800g for 20-40min and then filter with a 0.22-0.45nm filter membrane. Add 0.01-0.5% antioxidant, stand at 0-10°C for 8-12h

[0063] (3) Rough extraction: centrifuge the sample in (2) at 200-800g for 20-40min. Stratification occurs after centrifugation, remove the top 1-3.0ml sample, and carefully absorb 2-4.5ml of the remaining sample. The aspirated sample is then mixed. Repeat the above operation 2-5 times.

[0064] (4) Blocking process: Take the above (3) sample in a centrifuge tube and add 2-5 times the volume of cell blocking agent. The temperature is 4-10°C, the centrifugal force is 400-900g, and the centrifugal force is 15-45min. Remove the 2.5-4.5ml sample above the centrifuge tube, and carefully pipette the remaining 2-3ml sample. Repeat the above operation 1-3 times.

[0065] (5) Di...

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PUM

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Abstract

The invention discloses a preparation method of animal peripheral blood erythrocytes, relates to the technical field of erythrocyte preparation, and in particular, relates to a preparation method of animal erythrocytes. At present, many methods for preparing the animal peripheral blood erythrocytes exist. However, the extraction rate is low, time is consumed, and the cost is high. The effective preservation time of the prepared erythrocytes is short, so that frequent preparation is needed and waste of the mouse erythrocytes is caused. The invention aims to provide an erythrocyte extraction process and an erythrocyte preservation method which are simple and convenient to operate, low in cost, high in stability and high in extraction rate. The invention provides the erythrocyte extraction process which comprises the following six steps: (1) preparation, (2) pretreatment, (3) crude extraction, (4) blocking process, (5) dissolution process and (6) preservation.

Description

technical field [0001] The invention relates to the technical field of red blood cell preparation, in particular to a method for preparing animal red blood cells. Background technique [0002] Red blood cells contain hemoglobin, which makes blood red. Iron is contained in hemoglobin, so people with anemia should eat more iron-rich foods and protein to replenish blood. Hemoglobin can combine with oxygen in the air, so red blood cells can transport the oxygen inhaled into the alveoli to the tissue through hemoglobin, and part of the carbon dioxide produced by the metabolism in the tissue is also transported to the lungs through the red blood cells and exchange gas with the oxygen outside the body through the alveoli. Carbon dioxide is excreted from the body. [0003] Red blood cells are red because they are rich in hemoglobin. Hemoglobin contains iron, which is easy to combine with oxygen in places with high oxygen content, and easy to separate from oxygen in places with lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078A01N1/02
CPCC12N5/0641A01N1/021A01N1/0231C12N2509/10C12N2509/00
Inventor 李昊徐李鹏刘玲
Owner JILIN GETEIN BIOTECH CO LTD
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