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A method for establishing the fingerprint of Muli Powder and its fingerprint

A fingerprint and method establishment technology, applied in the field of quality identification of traditional Chinese medicine, can solve the problems of no oyster powder fingerprint research, complex components of oyster powder, qualitative and quantitative analysis is difficult to fully reflect the comprehensive information of the compound, to achieve large peak area, reproducible Good performance and moderate retention time

Active Publication Date: 2022-08-05
QINGDAO NAT LAB FOR MARINE SCI & TECH DEV CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the composition of oyster powder is complex, and the qualitative and quantitative analysis of one or two components is difficult to fully reflect the comprehensive information of the compound, and so far there is no related research on the fingerprint of oyster powder

Method used

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  • A method for establishing the fingerprint of Muli Powder and its fingerprint
  • A method for establishing the fingerprint of Muli Powder and its fingerprint
  • A method for establishing the fingerprint of Muli Powder and its fingerprint

Examples

Experimental program
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Effect test

preparation example Construction

[0036] 2. Preparation of oyster powder

[0037] A total of 15 batches of prescription decoction pieces were purchased from major decoction pieces companies across the country, and the origin information is shown in Table 1-Table 4.

[0038] Table 1. Sources of astragalus pieces

[0039]

[0040] Table 2. Sources of Ephedra Root Pieces

[0041]

[0042] Table 3. Sources of floating wheat pieces

[0043]

[0044] Table 4. Sources of Ephedra Root Pieces

[0045] medicinal herbs Numbering Origin Manufacturer Calcined Oyster 1 Liaoning Anhui Wansheng Chinese Herbal Pieces Co., Ltd.

[0046] The preparation method of the oyster powder of the present invention is as follows: take 3 parts of calcined oyster, 3 parts of astragalus, 3 parts of ephedra root and 2.5 parts of floating wheat, the above four flavors, soak in water for 1 hour, and decoct twice for 2 hours each time , add water 8 times the weight of the whole decoction piece twice, fi...

Embodiment 1

[0050] The establishment method of the fingerprint of oyster powder described in embodiment 1, comprises the following steps:

[0051] Step 1: Take the reference substance of verbascoside, formononetin, and verbasco, and add methanol to prepare the concentrations of 0.22, 0.31, and 0.28 mg·mL, respectively. −1 mixed reference solution.

[0052] Step 2: Take 15 batches of oyster powder for testing (batch numbers 20200801-20200815), take about 50 mg, accurately weigh, add 2 mL of water to dissolve; Cleanert S C18 solid phase extraction column treatment, the treatment process is: methanol activation, Equilibrated with water, loaded, washed with 2 column volumes of aqueous solution and 2 column volumes of 0.4% formic acid methanol respectively, collected the eluate, N 2 Blow dry, add 200 to the residue μ L methanol was reconstituted, centrifuged, and the supernatant was taken to prepare 15 batches of oyster powder test solution.

[0053] Step 3: Precisely draw each test solutio...

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Abstract

The invention discloses a method for establishing a fingerprint of Muli Powder and the fingerprint thereof. The method for establishing the fingerprint spectrum comprises the following steps: step 1: preparation of the reference peak solution; step 2: preparation of the test solution of Oyster Powder; step 3: respectively accurately drawing the quantitative reference peak solution and the test solution and injecting into the liquid phase Chromatograph, record the chromatogram; Step 4: Export the oyster powder fingerprint instrument obtained in step 3, and import it into the Chinese medicine chromatographic fingerprint similarity evaluation system, and select the chromatogram that exists in the chromatogram of different batches of oyster powder for the test. The peaks were taken as common peaks, and the average value calculation method was used to generate the control fingerprint of Mulisan, and the relative retention time and relative peak area of ​​each common peak were calculated. The method for establishing the fingerprint spectrum of the invention has the advantages of simplicity, stability, high precision, good reproducibility and the like. The fingerprint of Muli Powder provided by the invention can comprehensively and objectively characterize the quality of Muli Powder, which is beneficial to comprehensively monitor the quality of medicines.

Description

technical field [0001] The invention relates to a method for establishing a fingerprint of Muli powder and a fingerprint of Muli powder established by the method, belonging to the technical field of quality identification of traditional Chinese medicines. Background technique [0002] Traditional Chinese medicine fingerprint is an effective means of comprehensive analysis to identify the authenticity of Chinese herbal medicines or Chinese patent medicines by using modern analytical techniques, and to evaluate the consistency and stability of the quality of raw materials, semi-finished products and finished products. Compared with the existing identification of a single effective component or index component, the traditional Chinese medicine fingerprint technology integrates various effective chemical information, making the results more scientific and reasonable. At present, the fingerprint of traditional Chinese medicine has become an important method for quality control of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8686G01N30/8634
Inventor 马晓青魏宝红胡淑曼刘红兵管华诗
Owner QINGDAO NAT LAB FOR MARINE SCI & TECH DEV CENT
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